##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062598_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1902104
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202170859216952	32.0	32.0	32.0	32.0	32.0
2	30.803551225379895	32.0	32.0	32.0	32.0	32.0
3	30.78530984635961	32.0	32.0	32.0	32.0	32.0
4	30.88555567939503	32.0	32.0	32.0	32.0	32.0
5	30.64230557319684	32.0	32.0	32.0	32.0	32.0
6	34.300629197982865	36.0	36.0	36.0	32.0	36.0
7	34.20054213649727	36.0	36.0	36.0	32.0	36.0
8	34.190056905405804	36.0	36.0	36.0	32.0	36.0
9	34.38462618237489	36.0	36.0	36.0	32.0	36.0
10	34.007469623112094	36.0	36.0	36.0	32.0	36.0
11	34.34584439126357	36.0	36.0	36.0	32.0	36.0
12	34.104850733713825	36.0	36.0	36.0	32.0	36.0
13	34.24346197684249	36.0	36.0	36.0	32.0	36.0
14	34.14146071928769	36.0	36.0	36.0	32.0	36.0
15	34.06587547263452	36.0	36.0	36.0	32.0	36.0
16	34.06930798736557	36.0	36.0	36.0	32.0	36.0
17	33.99485885104074	36.0	36.0	36.0	32.0	36.0
18	34.003265857177105	36.0	36.0	36.0	32.0	36.0
19	33.99107882639435	36.0	36.0	36.0	32.0	36.0
20	33.991298057309166	36.0	36.0	36.0	32.0	36.0
21	33.974646496721526	36.0	36.0	36.0	32.0	36.0
22	33.92034136934679	36.0	36.0	36.0	32.0	36.0
23	33.88671439626855	36.0	36.0	36.0	32.0	36.0
24	33.85181935372619	36.0	36.0	36.0	32.0	36.0
25	33.39101331998671	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	58.0
5	195.0
6	312.0
7	93.0
8	261.0
9	344.0
10	209.0
11	76.0
12	136.0
13	105.0
14	284.0
15	407.0
16	598.0
17	778.0
18	1141.0
19	1459.0
20	2300.0
21	3535.0
22	5160.0
23	7811.0
24	11169.0
25	15930.0
26	22709.0
27	29748.0
28	40431.0
29	53964.0
30	70371.0
31	96608.0
32	138279.0
33	200801.0
34	438618.0
35	758202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69121618211185	17.197489736859815	10.695275169930682	26.416018911097648
2	17.343701121687445	19.893535641901217	36.08642277128038	26.67634046513095
3	19.379767932156696	23.23691003822232	28.148925095818676	29.234396933802305
4	12.922507754377497	14.894083537478922	35.37904827405619	36.8043604340874
5	14.926315866977802	36.036723997292555	33.58687974873608	15.450080386993559
6	34.92931432953545	34.84447469380413	16.495654927273552	13.730556049386871
7	30.61757336087199	30.090438821091485	20.68355987317206	18.608427944864463
8	28.428244815057447	32.57304171895677	19.47538878847434	19.52332467751144
9	27.410933515477904	14.078298309444397	18.407248876769305	40.10351929830839
10	15.88657349900839	26.479201492254507	31.71993490334452	25.914290105392578
11	37.884532991701676	20.850864939349503	21.99429233718371	19.27030973176511
12	24.594689494012663	23.510931025751304	28.61391887630666	23.280460603929367
13	29.599736824791105	19.16057635370748	25.108388709783537	26.131298111717875
14	23.287027338293086	19.478520664524016	24.73031740801526	32.50413458916764
15	25.27318794888772	27.084528525127187	21.974511230153677	25.667772295831416
16	26.086183076538465	25.526716753726923	23.547584900198682	24.839515269535926
17	24.32209652663294	25.67711392820824	25.033055623982804	24.967733921176023
18	25.144985326437418	24.648900469817207	25.93385710713675	24.27225709660863
19	25.67683378130965	24.988971675894664	24.98518151200166	24.34901303079403
20	25.665965728602746	24.347640716167458	24.794311084910007	25.192082470319793
21	26.53559368428256	24.236341958356693	24.210232641383794	25.017831715976957
22	25.860038277159276	24.43509599564051	24.573515048925792	25.131350678274423
23	24.49361114284496	24.22659055456786	25.311414340458622	25.96838396212856
24	24.900571329904196	24.885357624724744	24.962794857662367	25.251276187708694
25	24.880217550826604	24.630808961864386	25.006448406953965	25.482525080355046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	195.0
1	195.0
2	512.5
3	830.0
4	830.0
5	830.0
6	1973.5
7	3117.0
8	3117.0
9	3117.0
10	3568.5
11	4020.0
12	4020.0
13	4020.0
14	4815.0
15	5610.0
16	5610.0
17	5610.0
18	8911.5
19	12213.0
20	12213.0
21	12213.0
22	19289.0
23	26365.0
24	26365.0
25	26365.0
26	39054.5
27	51744.0
28	51744.0
29	51744.0
30	64511.5
31	77279.0
32	77279.0
33	77279.0
34	96250.0
35	115221.0
36	115221.0
37	115221.0
38	132171.5
39	149122.0
40	149122.0
41	149122.0
42	170810.0
43	192498.0
44	192498.0
45	192498.0
46	218398.0
47	244298.0
48	244298.0
49	244298.0
50	248823.5
51	253349.0
52	253349.0
53	253349.0
54	238782.5
55	224216.0
56	224216.0
57	224216.0
58	206522.5
59	188829.0
60	188829.0
61	188829.0
62	167364.5
63	145900.0
64	145900.0
65	145900.0
66	121703.0
67	97506.0
68	97506.0
69	97506.0
70	74180.5
71	50855.0
72	50855.0
73	50855.0
74	39739.5
75	28624.0
76	28624.0
77	28624.0
78	23149.0
79	17674.0
80	17674.0
81	17674.0
82	12398.5
83	7123.0
84	7123.0
85	7123.0
86	5274.5
87	3426.0
88	3426.0
89	3426.0
90	2252.0
91	1078.0
92	1078.0
93	1078.0
94	653.0
95	228.0
96	228.0
97	228.0
98	506.0
99	784.0
100	784.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00799115085189874
2	0.0031018282911975366
3	0.00389042870421386
4	0.014457674238632588
5	0.035592165307470044
6	0.04573882395494673
7	0.0662950080542389
8	0.08669347207092777
9	0.09368572906633917
10	0.11355845947435052
11	0.11576654063079621
12	0.13201170913893248
13	0.1171860213742256
14	0.11976211605674558
15	0.11198125864831786
16	0.10930001724406237
17	0.11965696933501008
18	0.12770069354777655
19	0.12880473412599944
20	0.11928895580893578
21	0.12612349272174392
22	0.14715283706884585
23	0.13800507227785652
24	0.13148597553025493
25	0.12670179969128922
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1902104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.86148253616459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0104194897544	45.25362133574282
2	11.625973605214611	12.988882430272131
3	3.211061486237105	5.381239654079543
4	1.3452048556886738	3.005805502144666
5	0.69525619761732	1.9419020970680063
6	0.4446866421183808	1.490451305565696
7	0.2844099457202587	1.1121292851174625
8	0.20878254288072884	0.9330321898390295
9	0.1558937275651509	0.7837609265890451
>10	0.8506627744916286	8.64143222520456
>50	0.07850865566441614	3.0664544318487943
>100	0.07563791327950405	8.979659870876521
>500	0.010291859095983017	3.85891631380444
>1k	0.003210304672141491	2.5627124318473573
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2595	0.13642787145182386	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1973	0.10372724099208036	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1949	0.10246548033125424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.257336086775487E-5
2	0.0	0.0	0.0	0.0	5.257336086775487E-5
3	0.0	0.0	0.0	0.0	5.257336086775487E-5
4	0.0	0.0	0.0	5.257336086775487E-5	5.257336086775487E-5
5	0.0	0.0	0.0	5.257336086775487E-5	5.257336086775487E-5
6	0.0	0.0	0.0	5.257336086775487E-5	5.257336086775487E-5
7	0.0	0.0	0.0	1.0514672173550973E-4	5.257336086775487E-5
8	0.0	0.0	0.0	1.0514672173550973E-4	5.257336086775487E-5
9	0.0	0.0	0.0	1.577200826032646E-4	5.257336086775487E-5
10	0.0	0.0	0.0	1.577200826032646E-4	5.257336086775487E-5
11	0.0	0.0	0.0	2.1029344347101947E-4	5.257336086775487E-5
12	0.0	0.0	0.0	2.6286680433877435E-4	1.0514672173550973E-4
13	0.0	0.0	0.0	2.6286680433877435E-4	1.0514672173550973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	100	0.0	17.103413	15
CGTTATT	180	0.0	14.766339	2
GCGTTAT	190	0.0	13.987323	1
ACAGTCG	70	7.2614584E-6	13.571993	8
GTTATTC	225	0.0	13.503494	3
CAGTGCG	95	7.380913E-8	13.001225	9
CGACCAT	320	0.0	12.469925	10
TCGCGTA	155	1.8189894E-12	12.259219	9
ATCCCCG	265	0.0	12.190791	12
TAGAGTG	205	0.0	12.048013	5
ATCGCCA	270	0.0	11.966926	16
CGCATCG	280	0.0	11.877057	13
CGGTCCA	305	0.0	11.525676	10
TATTAGC	165	7.2759576E-12	11.506238	2
CGGAATT	100	1.9255021E-6	11.402275	15
AATCGTA	75	2.0709426E-4	11.401976	13
CAAGACG	335	0.0	11.340172	4
GTATTAG	335	0.0	11.333013	1
GTCGCGT	160	4.5474735E-11	11.281718	8
AATCCCC	280	0.0	11.196599	11
>>END_MODULE