FastQCFastQC Report
Thu 2 Feb 2017
SRR4062598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902104
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20210.1062507623137326No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19200.10094085286608934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27100.019.5685751
TAGGCCG901.0025065E-519.5554395
ATACGAA6650.019.5186840
CGCAATA6750.019.22951536
TACGAAT7100.018.2815841
TAGGACG4600.018.1738034
GGCGTTA5100.018.1175442
GTATTAG6000.017.6035981
TAACGGC2250.017.59989536
CAATACG7500.017.30656238
ACCGTCG4650.017.0321568
CTAGCGG7550.016.90056229
TAGCGGC7650.016.67963830
CGTCGTA4650.016.5590410
TCTAGCG7750.016.46441728
CGAATGC7750.016.46441743
CGTTATT5650.016.35388444
ATACCGT5000.016.2799036
CGGCGTT5700.016.2104341
GCAATAC8100.016.02459537
Produced by FastQC (version 0.11.5)