##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062598_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1902104
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.311374667210625	32.0	32.0	32.0	32.0	32.0
2	31.40567760753355	32.0	32.0	32.0	32.0	32.0
3	31.48154517313459	32.0	32.0	32.0	32.0	32.0
4	31.585084201494766	32.0	32.0	32.0	32.0	32.0
5	31.51327004201663	32.0	32.0	32.0	32.0	32.0
6	35.10904924231272	36.0	36.0	36.0	36.0	36.0
7	35.12720229808675	36.0	36.0	36.0	36.0	36.0
8	35.06345236643212	36.0	36.0	36.0	36.0	36.0
9	35.20045696765266	36.0	36.0	36.0	36.0	36.0
10	35.06404171380745	36.0	36.0	36.0	36.0	36.0
11	35.20556184099292	36.0	36.0	36.0	36.0	36.0
12	35.118630737330875	36.0	36.0	36.0	36.0	36.0
13	35.159345650921296	36.0	36.0	36.0	36.0	36.0
14	35.12017218827151	36.0	36.0	36.0	36.0	36.0
15	35.07459686746886	36.0	36.0	36.0	36.0	36.0
16	35.095774994427224	36.0	36.0	36.0	36.0	36.0
17	35.06757937526024	36.0	36.0	36.0	36.0	36.0
18	35.0822568061473	36.0	36.0	36.0	36.0	36.0
19	35.071385686587064	36.0	36.0	36.0	36.0	36.0
20	35.061767915949915	36.0	36.0	36.0	36.0	36.0
21	35.061111274672676	36.0	36.0	36.0	36.0	36.0
22	35.03952044683151	36.0	36.0	36.0	36.0	36.0
23	35.00331475040271	36.0	36.0	36.0	36.0	36.0
24	34.97642189911803	36.0	36.0	36.0	36.0	36.0
25	34.95193270189222	36.0	36.0	36.0	32.0	36.0
26	34.90998126285419	36.0	36.0	36.0	32.0	36.0
27	34.890286230405906	36.0	36.0	36.0	32.0	36.0
28	34.88428445552925	36.0	36.0	36.0	32.0	36.0
29	34.86178989161476	36.0	36.0	36.0	32.0	36.0
30	34.839457779385356	36.0	36.0	36.0	32.0	36.0
31	34.8443255468681	36.0	36.0	36.0	32.0	36.0
32	34.813540163944765	36.0	36.0	36.0	32.0	36.0
33	34.791677006094304	36.0	36.0	36.0	32.0	36.0
34	34.79064183661882	36.0	36.0	36.0	32.0	36.0
35	34.76460855978432	36.0	36.0	36.0	32.0	36.0
36	34.7414999390149	36.0	36.0	36.0	32.0	36.0
37	34.72945222763845	36.0	36.0	36.0	32.0	36.0
38	34.69966994444047	36.0	36.0	36.0	32.0	36.0
39	34.69558499430105	36.0	36.0	36.0	32.0	36.0
40	34.68365189285129	36.0	36.0	36.0	32.0	36.0
41	34.65441321820468	36.0	36.0	36.0	32.0	36.0
42	34.63735736847197	36.0	36.0	36.0	32.0	36.0
43	34.629710047400145	36.0	36.0	36.0	32.0	36.0
44	34.60073844542675	36.0	36.0	36.0	32.0	36.0
45	34.57212171363921	36.0	36.0	36.0	32.0	36.0
46	34.571934552474524	36.0	36.0	36.0	32.0	36.0
47	34.541520863212526	36.0	36.0	36.0	32.0	36.0
48	34.50952366432119	36.0	36.0	36.0	32.0	36.0
49	34.503849421482734	36.0	36.0	36.0	32.0	36.0
50	33.98526684135042	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	49.0
22	133.0
23	436.0
24	1190.0
25	2738.0
26	5693.0
27	10503.0
28	17748.0
29	27799.0
30	41381.0
31	60056.0
32	89281.0
33	150178.0
34	333708.0
35	1161196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07012890864081	17.78393447932814	11.22277070499082	26.923165907040232
2	16.646197459154468	20.339329689533997	35.912313279818974	27.102159571492564
3	18.724363780128638	23.57643733695601	28.4698337726737	29.229365110241663
4	12.688745036414959	15.291548354636946	35.6308780334188	36.388828575529296
5	14.65015301484725	36.38677821337751	33.43157547199074	15.531493299784502
6	35.17542208655915	35.022853712058996	16.235546223170417	13.566177978211428
7	30.669790226922515	30.136433200515427	20.631322278551686	18.562454294010365
8	28.448654752842117	32.72139693728629	19.332644271816893	19.497304038054704
9	27.511482022013517	13.78788962117739	18.318241273873564	40.38238708293553
10	15.802500809629757	26.565424393198267	31.725867775894486	25.90620702127749
11	38.054859250598284	20.783248444879984	21.87950816569441	19.282384138827318
12	24.6183174001001	23.451346508918544	28.561477185264316	23.368858905717037
13	29.768719270870577	19.015837199227803	25.144787035829797	26.070656494071827
14	23.43715170148425	19.30467524383525	24.782556579450965	32.475616475229536
15	25.408074427055517	27.049099313181614	21.815158371992275	25.727667887770593
16	26.173805428094365	25.475158035522767	23.499188267308202	24.851848269074665
17	24.421430163650356	25.663528387511935	24.924714947237376	24.990326501600332
18	25.3221695553976	24.52952099359446	25.890119572851955	24.258189878155978
19	25.79128165442058	24.912728220959526	25.00935805823446	24.286632066385433
20	25.828030433667138	24.207719451722934	24.797697707380877	25.166552407229048
21	26.655955720612546	24.14289649777299	24.245256831382513	24.955890950231954
22	25.932651421793974	24.316704028801787	24.650807737116374	25.09983681228787
23	24.60286083200498	24.100469795552716	25.304084319259097	25.99258505318321
24	24.95200052152774	24.774828295403406	25.023710585751356	25.249460597317498
25	25.154145094064255	24.389202693438424	25.06193141910222	25.394720793395102
26	24.84306851780975	25.044214196489783	25.3174379529195	24.79527933278096
27	25.541032456690065	24.624047896434682	24.65128089736418	25.18363874951107
28	24.72693396365288	24.49019611966538	25.398453502016714	25.384416414665022
29	24.809526713576126	24.77609005606423	25.231901094787666	25.182482135571977
30	24.807003192254474	24.828715990292853	25.467009164588266	24.897271652864408
31	25.249513170678366	24.670680467524384	24.599759003713782	25.480047358083468
32	24.980442709757195	24.666579745376698	24.689344010632436	25.66363353423367
33	24.716156424674992	24.262185453581928	25.180694641302477	25.840963480440603
34	25.312496056997936	24.44361612193655	25.39077779133002	24.853110029735493
35	25.830343661545317	24.379844635203966	25.150517532164386	24.639294171086334
36	24.743336852243623	24.826665629219015	25.047841758389655	25.38215576014771
37	25.721306511105595	24.578098779036267	24.646654441607822	25.05394026825032
38	24.80726605905881	24.439935986675806	25.286997976977073	25.465799977288306
39	25.3430411796621	24.312445586571503	24.75464012483019	25.58987310893621
40	25.645180284569086	24.37027628352603	25.255874547343364	24.728668884561518
41	24.639767331334145	24.7326118866266	25.63792516076934	24.98969562126992
42	25.684505158498165	24.82529872183645	25.121865050491454	24.368331069173927
43	24.882340818377966	23.95773312079676	25.4284728910722	25.73145316975307
44	24.532097088276984	24.599601283631177	25.156300601859837	25.712001026232006
45	24.653120964994553	24.754692698191054	25.285578496233647	25.306607840580746
46	24.6000873769717	24.314245863657224	25.481532808685962	25.60413395068511
47	24.89716650614267	24.172863313467612	25.68897389417193	25.240996286217786
48	25.322116982036736	24.952315961692946	24.833710459575293	24.89185659669503
49	24.498502710682487	24.818621905006246	25.179958614250324	25.502916770060942
50	24.306437986205783	25.144668973939872	25.183941336448484	25.364951703405865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	38.0
2	54.0
3	153.0
4	252.0
5	292.0
6	332.0
7	292.5
8	253.0
9	351.0
10	449.0
11	640.0
12	831.0
13	1407.5
14	1984.0
15	2630.0
16	3276.0
17	3725.5
18	4175.0
19	4449.0
20	4723.0
21	5646.5
22	6570.0
23	7344.5
24	8119.0
25	10010.5
26	11902.0
27	14972.0
28	18042.0
29	20790.5
30	23539.0
31	26170.0
32	28801.0
33	33372.0
34	37943.0
35	44287.5
36	50632.0
37	60187.5
38	69743.0
39	75456.0
40	81169.0
41	88969.0
42	96769.0
43	99120.0
44	101471.0
45	111842.0
46	122213.0
47	129930.0
48	137647.0
49	142150.0
50	146653.0
51	141526.5
52	136400.0
53	135210.5
54	134021.0
55	134762.5
56	135504.0
57	132159.0
58	128814.0
59	118308.5
60	107803.0
61	95468.5
62	83134.0
63	73123.0
64	63112.0
65	53880.0
66	44648.0
67	39542.0
68	34436.0
69	31365.0
70	28294.0
71	22259.0
72	16224.0
73	14471.0
74	12718.0
75	9484.0
76	6250.0
77	5540.5
78	4831.0
79	4148.0
80	3465.0
81	2745.5
82	2026.0
83	1689.5
84	1353.0
85	1137.5
86	922.0
87	658.0
88	394.0
89	259.0
90	124.0
91	79.5
92	35.0
93	26.5
94	18.0
95	12.5
96	7.0
97	5.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015351421373384421
2	0.0031544016520652918
3	5.257336086775487E-4
4	5.257336086775487E-5
5	5.257336086775487E-5
6	3.154401652065292E-4
7	5.257336086775487E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.257336086775487E-5
47	0.0
48	0.0
49	0.0
50	1.577200826032646E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1902104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.51303475826638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.441431009552	47.653852834248696
2	11.618364021395667	13.596514756362325
3	3.0876170308091853	5.419975279318592
4	1.259853191858184	2.94871334422043
5	0.6543580086710361	1.9144236452859156
6	0.4094582928522672	1.4375188393035072
7	0.2869959024228744	1.1755100849764772
8	0.19495994408583778	0.9126158387811436
9	0.14298359250752388	0.7529763526429087
>10	0.7599002744552712	8.09430392319257
>50	0.06871897926305369	2.8296626178363162
>100	0.06504753875757453	8.186754183714061
>500	0.007779389033327586	3.0585443331866786
>1k	0.0025328243364322375	2.0186339669304068
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2021	0.1062507623137326	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1920	0.10094085286608934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.257336086775487E-5
7	0.0	0.0	0.0	5.257336086775487E-5	5.257336086775487E-5
8	0.0	0.0	0.0	5.257336086775487E-5	5.257336086775487E-5
9	0.0	0.0	0.0	2.1029344347101947E-4	1.0514672173550973E-4
10	0.0	0.0	0.0	2.1029344347101947E-4	2.1029344347101947E-4
11	0.0	0.0	0.0	2.1029344347101947E-4	2.1029344347101947E-4
12	0.0	0.0	0.0	3.154401652065292E-4	4.2058688694203894E-4
13	0.0	0.0	0.0	3.154401652065292E-4	4.2058688694203894E-4
14	0.0	5.257336086775487E-5	0.0	3.154401652065292E-4	5.257336086775487E-4
15	0.0	5.257336086775487E-5	0.0	3.154401652065292E-4	5.257336086775487E-4
16	0.0	5.257336086775487E-5	0.0	3.154401652065292E-4	5.257336086775487E-4
17	0.0	5.257336086775487E-5	0.0	3.154401652065292E-4	5.257336086775487E-4
18	0.0	5.257336086775487E-5	0.0	3.6801352607428406E-4	6.308803304130584E-4
19	0.0	5.257336086775487E-5	0.0	5.257336086775487E-4	6.308803304130584E-4
20	0.0	5.257336086775487E-5	0.0	5.783069695453035E-4	6.308803304130584E-4
21	0.0	5.257336086775487E-5	0.0	8.937471347518327E-4	6.308803304130584E-4
22	0.0	5.257336086775487E-5	0.0	0.0011040405782228521	7.360270521485681E-4
23	0.0	5.257336086775487E-5	0.0	0.0014720541042971362	7.360270521485681E-4
24	0.0	5.257336086775487E-5	0.0	0.0021555077955779495	7.360270521485681E-4
25	0.0	5.257336086775487E-5	0.0	0.0026286680433877433	7.360270521485681E-4
26	0.0	5.257336086775487E-5	0.0	0.0033646950955363115	7.360270521485681E-4
27	0.0	5.257336086775487E-5	0.0	0.004679029117230183	8.411737738840779E-4
28	0.0	5.257336086775487E-5	0.0	0.009358058234460367	8.411737738840779E-4
29	0.0	5.257336086775487E-5	0.0	0.02087162426449868	8.411737738840779E-4
30	0.0	5.257336086775487E-5	0.0	0.043583316159368786	8.411737738840779E-4
31	0.0	5.257336086775487E-5	0.0	0.08979530036212531	8.411737738840779E-4
32	0.0	5.257336086775487E-5	0.0	0.143262408364632	9.463204956195875E-4
33	0.0	5.257336086775487E-5	0.0	0.1976758368627583	9.463204956195875E-4
34	0.0	5.257336086775487E-5	0.0	0.26071129654319636	9.463204956195875E-4
35	0.0	5.257336086775487E-5	0.0	0.33704781652317645	9.463204956195875E-4
36	0.0	5.257336086775487E-5	0.0	0.4477147411498004	9.463204956195875E-4
37	0.0	5.257336086775487E-5	0.0	0.605014236866123	9.463204956195875E-4
38	0.0	5.257336086775487E-5	0.0	0.8115749717155318	9.988938564873425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2710	0.0	19.568575	1
TAGGCCG	90	1.0025065E-5	19.555439	5
ATACGAA	665	0.0	19.51868	40
CGCAATA	675	0.0	19.229515	36
TACGAAT	710	0.0	18.28158	41
TAGGACG	460	0.0	18.173803	4
GGCGTTA	510	0.0	18.11754	42
GTATTAG	600	0.0	17.603598	1
TAACGGC	225	0.0	17.599895	36
CAATACG	750	0.0	17.306562	38
ACCGTCG	465	0.0	17.032156	8
CTAGCGG	755	0.0	16.900562	29
TAGCGGC	765	0.0	16.679638	30
CGTCGTA	465	0.0	16.55904	10
TCTAGCG	775	0.0	16.464417	28
CGAATGC	775	0.0	16.464417	43
CGTTATT	565	0.0	16.353884	44
ATACCGT	500	0.0	16.279903	6
CGGCGTT	570	0.0	16.21043	41
GCAATAC	810	0.0	16.024595	37
>>END_MODULE