FastQCFastQC Report
Thu 2 Feb 2017
SRR4062597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120861
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32210.1518722820590317No Hit
GCGCAAGACGGACCAGAGCGAAAGC30480.14371521754608152No Hit
GTCTTGCGCCGGTCCAAGAATTTCA29420.13871724738207739No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27200.1282498004348234No Hit
GAATAGGACCGCGGTTCTATTTTGT26800.1263637739578407No Hit
GAATAACGCCGCCGCATCGCCAGTC26390.12443059681893345No Hit
GATTAAGAGGGACGGCCGGGGGCAT26270.12386478887583864No Hit
GAACTACGACGGTATCTGATCGTCT24750.11669788826330438No Hit
GGGTAGGCACACGCTGAGCCAGTCA24420.11514191641979366No Hit
GTATCTGATCGTCTTCGAACCTCCG23960.11297298597126357No Hit
GTCCTATTCCATTATTCCTAGCTGC23770.11207712339469678No Hit
TATCAACGCAGAGTACTTTTTTTTT23410.11037969956541235No Hit
CTATTGGAGCTGGAATTACCGCGGC22860.10778641315956114No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG21720.10241123770016046No Hit
ATCAGATACCGTCGTAGTTCCGACC21700.10231693637631131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG4600.013.01342513
GCATCGC4550.012.73876514
CAAGACG6050.012.7167024
AAGACGG6350.012.714535
GTCCTAG2250.012.6583941
ATCGCCA4750.012.40243516
CTATACT1001.4467878E-712.3477614
ACTCTAA3800.012.00065610
CGCAAGA6200.011.9457792
GCGCAAG6550.011.8853621
AACCGCG1850.011.8111777
AATCGTA1052.7145325E-711.764214513
ACGGTAT4350.011.5753459
CGCCAGT5000.011.40304718
CCGCATC5500.011.40116112
GTCCTAT5850.011.3600971
GCAAGAC7550.011.31977753
GTCTTAG2350.011.3117561
CGAACGA1850.011.29951616
CGTCTTA1104.9582377E-711.22947815
Produced by FastQC (version 0.11.5)