##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062597_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2120861
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176572156308218	32.0	32.0	32.0	32.0	32.0
2	30.790044232035953	32.0	32.0	32.0	32.0	32.0
3	30.775122933563303	32.0	32.0	32.0	32.0	32.0
4	30.861635911075737	32.0	32.0	32.0	32.0	32.0
5	30.641505501774986	32.0	32.0	32.0	32.0	32.0
6	34.28505639926426	36.0	36.0	36.0	32.0	36.0
7	34.176469367865224	36.0	36.0	36.0	32.0	36.0
8	34.169566039452846	36.0	36.0	36.0	32.0	36.0
9	34.348903110576316	36.0	36.0	36.0	32.0	36.0
10	33.987307041809906	36.0	36.0	36.0	32.0	36.0
11	34.31586086971282	36.0	36.0	36.0	32.0	36.0
12	34.07367008021742	36.0	36.0	36.0	32.0	36.0
13	34.21838536330292	36.0	36.0	36.0	32.0	36.0
14	34.11616791482327	36.0	36.0	36.0	32.0	36.0
15	34.042712370117606	36.0	36.0	36.0	32.0	36.0
16	34.04156425149974	36.0	36.0	36.0	32.0	36.0
17	33.96236245562533	36.0	36.0	36.0	32.0	36.0
18	33.9752336433175	36.0	36.0	36.0	32.0	36.0
19	33.96706620565893	36.0	36.0	36.0	32.0	36.0
20	33.9690206005957	36.0	36.0	36.0	32.0	36.0
21	33.94401754759034	36.0	36.0	36.0	32.0	36.0
22	33.89901506982306	36.0	36.0	36.0	32.0	36.0
23	33.85157773187399	36.0	36.0	36.0	32.0	36.0
24	33.81620247625846	36.0	36.0	36.0	32.0	36.0
25	33.37530700974746	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	14.0
4	82.0
5	190.0
6	344.0
7	102.0
8	325.0
9	357.0
10	261.0
11	78.0
12	141.0
13	103.0
14	355.0
15	451.0
16	689.0
17	930.0
18	1236.0
19	1712.0
20	2732.0
21	4120.0
22	6534.0
23	9513.0
24	13340.0
25	19006.0
26	26391.0
27	34093.0
28	45629.0
29	60526.0
30	77711.0
31	106371.0
32	152460.0
33	220811.0
34	486731.0
35	847522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72601773383405	17.09281687298297	10.884183087407317	26.296982305775664
2	17.51222063005499	19.600584496692047	35.78500367078634	27.10219120246662
3	19.126909805230753	22.947793391633024	28.177554713721364	29.747742089414864
4	12.923165723835734	14.76027132404398	34.99125704293763	37.325305909182646
5	15.249253583506672	35.81138892426408	33.08490517090933	15.85445232131992
6	35.38513210082004	34.469615490571236	16.279477861178098	13.865774547430629
7	31.06222381171213	29.92798226733186	20.40332572454161	18.6064681964144
8	28.84005068392626	31.96820220715757	19.25734362427826	19.93440348463791
9	27.381258023106547	14.031186287095068	18.46607641773012	40.121479272068264
10	16.263547134042692	26.08465580992024	30.83060766027028	26.821189395766787
11	38.18198469015888	20.82161051673546	21.510959338663202	19.48544545444246
12	24.87147612777367	23.4654917711201	27.87531590976583	23.7877161913404
13	29.48345686378826	18.733923377916334	25.110662863115103	26.671956895180305
14	23.69917664836734	19.37361828604447	24.33917309833909	32.5880319672491
15	25.611635013797486	26.707739714194272	21.712559157366858	25.968066114641385
16	26.462902543652948	25.31436922240348	23.078713144220895	25.144015089722675
17	24.595993738327817	25.51758876524583	24.638480959115963	25.247936537310387
18	25.71082853908247	24.110289958936097	25.484075344567604	24.694806157413833
19	25.853276238946616	24.812499704928403	24.678041479984703	24.656182576140274
20	25.945182484301636	23.87368802676015	24.367085321100053	25.814044167838162
21	27.29261558914395	23.853220104693058	23.794349143042744	25.05981516312025
22	26.199649158428816	23.862562654496603	24.276678479434686	25.661109707639895
23	24.670830691275615	23.62275806746324	25.105772803653288	26.600638437607856
24	25.045465704789244	24.753597597824445	24.485524626076447	25.715412071309867
25	25.204956633938124	24.209834047091515	24.562881143800833	26.022328175169523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	222.0
2	563.5
3	905.0
4	905.0
5	905.0
6	2253.5
7	3602.0
8	3602.0
9	3602.0
10	3906.5
11	4211.0
12	4211.0
13	4211.0
14	4834.0
15	5457.0
16	5457.0
17	5457.0
18	8682.0
19	11907.0
20	11907.0
21	11907.0
22	19100.5
23	26294.0
24	26294.0
25	26294.0
26	39485.5
27	52677.0
28	52677.0
29	52677.0
30	64633.0
31	76589.0
32	76589.0
33	76589.0
34	99505.0
35	122421.0
36	122421.0
37	122421.0
38	140046.0
39	157671.0
40	157671.0
41	157671.0
42	183854.0
43	210037.0
44	210037.0
45	210037.0
46	241737.0
47	273437.0
48	273437.0
49	273437.0
50	278857.0
51	284277.0
52	284277.0
53	284277.0
54	267893.0
55	251509.0
56	251509.0
57	251509.0
58	234881.5
59	218254.0
60	218254.0
61	218254.0
62	195193.0
63	172132.0
64	172132.0
65	172132.0
66	143103.5
67	114075.0
68	114075.0
69	114075.0
70	87124.5
71	60174.0
72	60174.0
73	60174.0
74	47685.0
75	35196.0
76	35196.0
77	35196.0
78	29196.5
79	23197.0
80	23197.0
81	23197.0
82	16320.5
83	9444.0
84	9444.0
85	9444.0
86	7013.0
87	4582.0
88	4582.0
89	4582.0
90	3052.5
91	1523.0
92	1523.0
93	1523.0
94	893.0
95	263.0
96	263.0
97	263.0
98	534.0
99	805.0
100	805.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007968461865251894
2	0.0029233410393231804
3	0.004479312882833906
4	0.014003746591596526
5	0.03446713386685879
6	0.04550038875720757
7	0.06520936544167674
8	0.08704012191275148
9	0.09340546127256807
10	0.11429320450515146
11	0.11716939488255004
12	0.1307016348549009
13	0.1174051481921729
14	0.12108289982228915
15	0.11122841148005456
16	0.11000249427001582
17	0.12150725577961027
18	0.12725963653440747
19	0.12876845771599366
20	0.11726369620639919
21	0.12584511667667048
22	0.14597844931846074
23	0.13569960501890507
24	0.131125990812222
25	0.1287213070540691
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2120861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.619585006337516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03465306946921	39.216666864269214
2	12.39770604890499	12.303380583544518
3	3.659823779114133	5.447968113479075
4	1.5292836221377957	3.035296747498643
5	0.8247018483708615	2.046068173506081
6	0.5215713689294639	1.5528092926480412
7	0.3388670934284825	1.1770111183757572
8	0.2704210935235307	1.0734545950078063
9	0.19222286027345215	0.8584216699949907
>10	1.0360779243035392	9.196681853811342
>50	0.08292707326507642	2.895025792082486
>100	0.08788994062861562	9.44296619063679
>500	0.015934657470707735	5.502786719320151
>1k	0.007919620180052349	6.251462285825146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3221	0.1518722820590317	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3048	0.14371521754608152	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2942	0.13871724738207739	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2720	0.1282498004348234	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2680	0.1263637739578407	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2639	0.12443059681893345	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2627	0.12386478887583864	No Hit
GAACTACGACGGTATCTGATCGTCT	2475	0.11669788826330438	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2442	0.11514191641979366	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2396	0.11297298597126357	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2377	0.11207712339469678	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2341	0.11037969956541235	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2286	0.10778641315956114	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2172	0.10241123770016046	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2170	0.10231693637631131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7150661924567425E-5
2	0.0	0.0	0.0	0.0	4.7150661924567425E-5
3	0.0	0.0	0.0	0.0	4.7150661924567425E-5
4	0.0	0.0	0.0	4.7150661924567425E-5	4.7150661924567425E-5
5	4.7150661924567425E-5	0.0	0.0	1.414519857737023E-4	4.7150661924567425E-5
6	4.7150661924567425E-5	0.0	0.0	1.414519857737023E-4	4.7150661924567425E-5
7	4.7150661924567425E-5	0.0	0.0	1.414519857737023E-4	4.7150661924567425E-5
8	4.7150661924567425E-5	0.0	0.0	1.414519857737023E-4	4.7150661924567425E-5
9	4.7150661924567425E-5	0.0	0.0	2.3575330962283714E-4	4.7150661924567425E-5
10	4.7150661924567425E-5	0.0	0.0	2.3575330962283714E-4	4.7150661924567425E-5
11	4.7150661924567425E-5	0.0	0.0	2.3575330962283714E-4	4.7150661924567425E-5
12	4.7150661924567425E-5	0.0	0.0	2.3575330962283714E-4	2.3575330962283714E-4
13	4.7150661924567425E-5	0.0	0.0	3.30054633471972E-4	3.30054633471972E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	460	0.0	13.013425	13
GCATCGC	455	0.0	12.738765	14
CAAGACG	605	0.0	12.716702	4
AAGACGG	635	0.0	12.71453	5
GTCCTAG	225	0.0	12.658394	1
ATCGCCA	475	0.0	12.402435	16
CTATACT	100	1.4467878E-7	12.347761	4
ACTCTAA	380	0.0	12.000656	10
CGCAAGA	620	0.0	11.945779	2
GCGCAAG	655	0.0	11.885362	1
AACCGCG	185	0.0	11.811177	7
AATCGTA	105	2.7145325E-7	11.7642145	13
ACGGTAT	435	0.0	11.575345	9
CGCCAGT	500	0.0	11.403047	18
CCGCATC	550	0.0	11.401161	12
GTCCTAT	585	0.0	11.360097	1
GCAAGAC	755	0.0	11.3197775	3
GTCTTAG	235	0.0	11.311756	1
CGAACGA	185	0.0	11.299516	16
CGTCTTA	110	4.9582377E-7	11.229478	15
>>END_MODULE