FastQCFastQC Report
Thu 2 Feb 2017
SRR4062597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120861
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29320.13824574076283172No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC29210.13772708348166146No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA27080.1276839924917286No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26840.12655237660553897No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA25980.12249741968002618No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG24300.11457610847669884No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA23630.11141701412775283No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC23530.11094550750850715No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC23090.1088708783838262No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG22250.10491022278216251No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA22200.10467446947253968No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT22140.1043915655009923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT1252.2906534E-819.35981422
GGCGCGT851.4315834E-418.11747221
ATACCGT7000.017.9141126
CTAGCGG8650.017.80329729
CGCAATA8600.017.65099336
ACCGTCG7150.017.5382928
TAGCGGC8800.017.4998330
TCTAGCG9100.017.40642528
GCGGATA1408.381721E-817.28554721
ATACGAA8850.017.1527840
GTATCAA39050.017.0183351
CGTCGTA7500.016.7198410
TACCGTC7500.016.719847
TACGAAT9100.016.68155141
GCAATAC9700.016.55654137
TATTTCG800.001989144416.4998425
CGAATGC9350.016.2355243
AATACGA9500.016.2103739
CGAGCCG10600.016.18852215
CAATACG9650.016.18637338
Produced by FastQC (version 0.11.5)