##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062597_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2120861 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30612378651878 32.0 32.0 32.0 32.0 32.0 2 31.42072865689925 32.0 32.0 32.0 32.0 32.0 3 31.493119539658657 32.0 32.0 32.0 32.0 32.0 4 31.589582721357033 32.0 32.0 32.0 32.0 32.0 5 31.530238898258773 32.0 32.0 32.0 32.0 32.0 6 35.120425148088444 36.0 36.0 36.0 36.0 36.0 7 35.12596393634472 36.0 36.0 36.0 36.0 36.0 8 35.06891965102852 36.0 36.0 36.0 36.0 36.0 9 35.198500514649474 36.0 36.0 36.0 36.0 36.0 10 35.0699946861204 36.0 36.0 36.0 36.0 36.0 11 35.21315871242859 36.0 36.0 36.0 36.0 36.0 12 35.11193048483611 36.0 36.0 36.0 36.0 36.0 13 35.159490886012804 36.0 36.0 36.0 36.0 36.0 14 35.1180647859525 36.0 36.0 36.0 36.0 36.0 15 35.07827198482126 36.0 36.0 36.0 36.0 36.0 16 35.09261898823166 36.0 36.0 36.0 36.0 36.0 17 35.056118246316004 36.0 36.0 36.0 36.0 36.0 18 35.07065903894692 36.0 36.0 36.0 36.0 36.0 19 35.06340962467602 36.0 36.0 36.0 36.0 36.0 20 35.055610905193696 36.0 36.0 36.0 36.0 36.0 21 35.04993726604431 36.0 36.0 36.0 36.0 36.0 22 35.03827454981727 36.0 36.0 36.0 36.0 36.0 23 35.00108729426398 36.0 36.0 36.0 36.0 36.0 24 34.96812756705885 36.0 36.0 36.0 32.0 36.0 25 34.9413558927247 36.0 36.0 36.0 32.0 36.0 26 34.900316428092175 36.0 36.0 36.0 32.0 36.0 27 34.89152094361677 36.0 36.0 36.0 32.0 36.0 28 34.87218492866812 36.0 36.0 36.0 32.0 36.0 29 34.84410906702514 36.0 36.0 36.0 32.0 36.0 30 34.83734294703896 36.0 36.0 36.0 32.0 36.0 31 34.84087594613697 36.0 36.0 36.0 32.0 36.0 32 34.802137433806365 36.0 36.0 36.0 32.0 36.0 33 34.78571957332423 36.0 36.0 36.0 32.0 36.0 34 34.77149799067454 36.0 36.0 36.0 32.0 36.0 35 34.74472961688673 36.0 36.0 36.0 32.0 36.0 36 34.72150508684916 36.0 36.0 36.0 32.0 36.0 37 34.71463806444647 36.0 36.0 36.0 32.0 36.0 38 34.678446630873026 36.0 36.0 36.0 32.0 36.0 39 34.67927931156262 36.0 36.0 36.0 32.0 36.0 40 34.67209732273827 36.0 36.0 36.0 32.0 36.0 41 34.64048610446418 36.0 36.0 36.0 32.0 36.0 42 34.604088622498125 36.0 36.0 36.0 32.0 36.0 43 34.60626132499961 36.0 36.0 36.0 32.0 36.0 44 34.579442971510154 36.0 36.0 36.0 32.0 36.0 45 34.53391664988889 36.0 36.0 36.0 32.0 36.0 46 34.550179384693294 36.0 36.0 36.0 32.0 36.0 47 34.52032641460237 36.0 36.0 36.0 32.0 36.0 48 34.48633408790109 36.0 36.0 36.0 32.0 36.0 49 34.47560071122059 36.0 36.0 36.0 32.0 36.0 50 33.96033969222877 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 2.0 20 16.0 21 51.0 22 186.0 23 517.0 24 1409.0 25 3315.0 26 6933.0 27 12878.0 28 21226.0 29 32231.0 30 46964.0 31 66645.0 32 98461.0 33 163218.0 34 364290.0 35 1302517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.196591309399906 17.718994530117836 11.391445781484833 26.692968378997428 2 16.90833921168478 20.012061622726776 35.55373127572172 27.525867889866728 3 18.52033120635895 23.23018144114656 28.57185292898659 29.677634423507897 4 12.630242345772032 15.169973982178877 35.26621823095791 36.93356544109118 5 14.940064436094586 36.07341546664303 33.05412283030335 15.932397266959034 6 35.65587587246817 34.66202953437087 16.004188884556246 13.677905708604706 7 31.082438256179096 30.014805314825114 20.368624048734947 18.534132380260836 8 28.853941866062886 32.13718390785629 19.117848835920885 19.89102539015994 9 27.49708726785961 13.803544881064814 18.415256822582904 40.284111028492674 10 16.318089681501995 26.015660620851627 30.91942376233049 26.746825935315893 11 38.40147939916855 20.611628956353105 21.54624937702188 19.44064226745647 12 24.840524673705634 23.41289693195358 27.92233908775728 23.824239306583507 13 29.683416310639878 18.533746435999344 25.13469765345301 26.648139599907772 14 23.823814950626186 19.23129332851139 24.326252404094376 32.61863931676805 15 25.65509950911446 26.694630152565395 21.692746483621512 25.957523854698632 16 26.643330232391467 25.204952139720614 22.99457625935882 25.1571413685291 17 24.751032717372805 25.419676254125097 24.578979952010055 25.25031107649205 18 25.878782249284605 24.008409792060867 25.519211301447854 24.593596657206675 19 26.010898403997246 24.694876279020644 24.69855403065076 24.595671286331353 20 26.044139620654068 23.66364415206843 24.441960128457264 25.850256098820246 21 27.321168148219048 23.822070376134977 23.855547346101417 25.001214129544557 22 26.336709477896004 23.707399966334428 24.413198224683278 25.542692331086293 23 24.70826706700722 23.39370661255028 25.083869239898327 26.814157080544177 24 25.17425705880772 24.541400874456176 24.534799781786738 25.749542284949367 25 25.40053308538372 23.869692544678788 24.728117495677463 26.001656874260032 26 25.150728878507362 24.766450983822136 25.020357298285933 25.06246283938457 27 26.070543991331824 24.043112679237346 24.21780588166787 25.66853744776296 28 25.042801013362027 24.00657091624581 25.2335254408469 25.717102629545263 29 24.9667469956777 24.41673452432762 25.130736997851344 25.485781482143338 30 24.938833803818355 24.469354663035435 25.4049180969427 25.186893436203505 31 25.581355873864435 24.331769031539547 24.118035081035487 25.96884001356053 32 25.331504516326152 24.188336717965015 24.082106276648965 26.39805248905987 33 24.902669246122212 23.7760513300966 24.828831309548338 26.492448114232854 34 25.751098256792876 23.988323610081 24.98471139787096 25.275866735255164 35 26.377447649798828 23.805426192475604 24.91917197779581 24.897954179929755 36 25.063217249975363 24.563278781589176 24.443516100300773 25.929987868134685 37 26.23981486764102 24.213420870108884 24.348979023142018 25.19778523910808 38 25.099853314290755 23.85856498846459 25.025496720435708 26.016084976808944 39 25.7789642979903 23.853095511681342 24.374298928595508 25.993641261732854 40 25.90806281033976 24.104502841063134 24.959910149698636 25.027524198898465 41 24.761122959024657 24.533243809943226 25.370356661751998 25.33527656928012 42 26.342980515931973 24.517825543493892 24.70878572428839 24.430408216285745 43 25.390678597041482 23.3426895963479 24.949725606722932 26.316906199887686 44 25.00988985133868 24.032220876332772 24.7347185883469 26.223170683981646 45 24.956986808659313 24.400137491330174 24.924028495974042 25.71884720403647 46 24.83237900168611 23.95075384656007 25.25833908572765 25.958528066026172 47 24.945069452957764 23.795064266382504 25.625736729439947 25.634129551219786 48 25.90773275570629 24.698176825355365 24.34053905465752 25.05355136428083 49 24.569031162343975 24.76173591762968 24.83863864722865 25.830594272797697 50 24.62246447195949 24.918476467093537 24.792914195184974 25.666144865762003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16.0 1 20.5 2 25.0 3 107.5 4 190.0 5 224.0 6 258.0 7 248.5 8 239.0 9 316.0 10 393.0 11 622.5 12 852.0 13 1514.0 14 2176.0 15 2846.5 16 3517.0 17 4074.0 18 4631.0 19 4955.0 20 5279.0 21 6126.0 22 6973.0 23 7797.5 24 8622.0 25 9808.0 26 10994.0 27 13533.0 28 16072.0 29 18698.5 30 21325.0 31 23739.5 32 26154.0 33 30297.5 34 34441.0 35 40525.5 36 46610.0 37 57638.5 38 68667.0 39 76516.0 40 84365.0 41 93726.0 42 103087.0 43 107224.5 44 111362.0 45 124158.5 46 136955.0 47 147649.0 48 158343.0 49 164068.5 50 169794.0 51 161979.5 52 154165.0 53 152921.5 54 151678.0 55 154802.5 56 157927.0 57 154407.5 58 150888.0 59 139293.5 60 127699.0 61 112154.5 62 96610.0 63 84869.5 64 73129.0 65 63081.5 66 53034.0 67 46998.0 68 40962.0 69 38233.5 70 35505.0 71 27435.0 72 19365.0 73 17427.0 74 15489.0 75 11194.0 76 6899.0 77 6329.0 78 5759.0 79 4953.0 80 4147.0 81 3353.0 82 2559.0 83 2179.0 84 1799.0 85 1508.0 86 1217.0 87 850.0 88 483.0 89 306.0 90 129.0 91 87.5 92 46.0 93 30.5 94 15.0 95 11.0 96 7.0 97 7.0 98 7.0 99 5.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016031225054352925 2 0.004007806263588231 3 3.772052953965394E-4 4 3.3005463347197196E-4 5 0.0 6 4.7150661924567433E-4 7 4.7150661924567425E-5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 2.3575330962283717E-4 47 4.7150661924567425E-5 48 0.0 49 0.0 50 4.7150661924567425E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2120861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.42741687556419 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77840677350716 41.82575789582994 2 12.129788452104039 12.718669515817258 3 3.4813257901851684 5.475507554450722 4 1.454633028729467 3.0505060879265726 5 0.7841610801067396 2.055576992217436 6 0.5150049900202013 1.6200228782870922 7 0.33558937358136093 1.231585879143162 8 0.23113533004160614 0.9694262642209921 9 0.1737959108464327 0.8200503602292886 >10 0.9450435892758142 8.9133031601784 >50 0.07433291369792493 2.7270599517672416 >100 0.07672412888770429 8.617798076667707 >500 0.013914551389858013 4.988244043823524 >1k 0.0061440876266905505 4.986491339440729 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2932 0.13824574076283172 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 2921 0.13772708348166146 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 2708 0.1276839924917286 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 2684 0.12655237660553897 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 2598 0.12249741968002618 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG 2430 0.11457610847669884 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA 2363 0.11141701412775283 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC 2353 0.11094550750850715 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC 2309 0.1088708783838262 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG 2225 0.10491022278216251 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA 2220 0.10467446947253968 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT 2214 0.1043915655009923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.414519857737023E-4 2 0.0 0.0 0.0 0.0 1.414519857737023E-4 3 0.0 0.0 0.0 0.0 1.414519857737023E-4 4 0.0 0.0 0.0 4.7150661924567425E-5 1.414519857737023E-4 5 0.0 0.0 0.0 1.414519857737023E-4 1.414519857737023E-4 6 0.0 0.0 0.0 1.414519857737023E-4 1.414519857737023E-4 7 0.0 0.0 0.0 1.414519857737023E-4 1.414519857737023E-4 8 0.0 0.0 0.0 1.414519857737023E-4 1.414519857737023E-4 9 0.0 0.0 0.0 1.886026476982697E-4 1.414519857737023E-4 10 0.0 0.0 0.0 1.886026476982697E-4 1.414519857737023E-4 11 0.0 0.0 0.0 1.886026476982697E-4 1.414519857737023E-4 12 0.0 0.0 0.0 1.886026476982697E-4 4.715066192456743E-4 13 0.0 0.0 0.0 1.886026476982697E-4 6.129586050193765E-4 14 0.0 0.0 0.0 1.886026476982697E-4 6.60109266943944E-4 15 0.0 0.0 0.0 1.886026476982697E-4 7.544105907930788E-4 16 0.0 0.0 0.0 1.886026476982697E-4 8.487119146422137E-4 17 0.0 0.0 0.0 1.886026476982697E-4 8.487119146422137E-4 18 0.0 0.0 0.0 2.829039715474046E-4 0.0010373145623404833 19 0.0 0.0 0.0 3.30054633471972E-4 0.0010373145623404833 20 0.0 0.0 0.0 4.2435595732110684E-4 0.001084465224265051 21 0.0 0.0 0.0 5.658079430948092E-4 0.001084465224265051 22 0.0 0.0 0.0 7.544105907930788E-4 0.001084465224265051 23 0.0 0.0 0.0 0.0011787665481141857 0.001084465224265051 24 0.0 0.0 0.0 0.0017917251531335623 0.001084465224265051 25 0.0 0.0 0.0 0.002451834420077506 0.001084465224265051 26 0.0 0.0 0.0 0.003206245010870585 0.0011787665481141857 27 0.0 0.0 0.0 0.004620764868607608 0.0011787665481141857 28 0.0 0.0 0.0 0.009430132384913486 0.001225917210038753 29 0.0 0.0 0.0 0.02088774323258337 0.001225917210038753 30 0.0 0.0 0.0 0.04319000632290376 0.001225917210038753 31 0.0 0.0 0.0 0.09444277583490855 0.001225917210038753 32 0.0 0.0 0.0 0.15526712971760054 0.001225917210038753 33 0.0 0.0 0.0 0.22094800177852297 0.001225917210038753 34 0.0 0.0 0.0 0.30336735882266685 0.001320218533887888 35 0.0 0.0 0.0 0.3946038896467048 0.0013673691958124554 36 0.0 0.0 0.0 0.5210619649283946 0.0013673691958124554 37 0.0 0.0 0.0 0.7082972434308519 0.0013673691958124554 38 0.0 0.0 0.0 0.9531506308051305 0.0013673691958124554 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGATAT 125 2.2906534E-8 19.359814 22 GGCGCGT 85 1.4315834E-4 18.117472 21 ATACCGT 700 0.0 17.914112 6 CTAGCGG 865 0.0 17.803297 29 CGCAATA 860 0.0 17.650993 36 ACCGTCG 715 0.0 17.538292 8 TAGCGGC 880 0.0 17.49983 30 TCTAGCG 910 0.0 17.406425 28 GCGGATA 140 8.381721E-8 17.285547 21 ATACGAA 885 0.0 17.15278 40 GTATCAA 3905 0.0 17.018335 1 CGTCGTA 750 0.0 16.71984 10 TACCGTC 750 0.0 16.71984 7 TACGAAT 910 0.0 16.681551 41 GCAATAC 970 0.0 16.556541 37 TATTTCG 80 0.0019891444 16.499842 5 CGAATGC 935 0.0 16.23552 43 AATACGA 950 0.0 16.21037 39 CGAGCCG 1060 0.0 16.188522 15 CAATACG 965 0.0 16.186373 38 >>END_MODULE