FastQCFastQC Report
Thu 2 Feb 2017
SRR4062596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517394
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG124272.4018446290447897No Hit
TCCATGTACTCTGCGTTGATACCAC97881.8917884629508652No Hit
GAGTACATGGAAGCAGTGGTATCAA54091.0454315280038036No Hit
CATGTACTCTGCGTTGATACCACTG42610.8235503310823086No Hit
GCTTCCATGTACTCTGCGTTGATAC34280.6625511699014678No Hit
GCGTTGATACCACTGCTTCCATGTA27590.5332493225665547No Hit
CATGGAAGCAGTGGTATCAACGCAG26020.502904942848197No Hit
CCCATGTACTCTGCGTTGATACCAC25980.5021318376324426No Hit
GTACATGGGAAGCAGTGGTATCAAC21830.42192217149793No Hit
GTATCAACGCAGAGTACATGGAAGC19300.3730232666014681No Hit
ACGCAGAGTACATGGAAGCAGTGGT17290.33417472950981264No Hit
ACTCTGCGTTGATACCACTGCTTCC17280.333981453205874No Hit
CAGTGGTATCAACGCAGAGTACATG15680.3030572445757005No Hit
TATCAACGCAGAGTACATGGAAGCA12710.24565418230594094No Hit
GTATCAACGCAGAGTACTTTTTTTT12700.24546090600200238No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11170.2158896314993989No Hit
ATACCACTGCTTCCATGTACTCTGC9050.17491505506441898No Hit
TATCAACGCAGAGTACTTTTTTTTT9000.17394867354472607No Hit
GGTATCAACGCAGAGTACATGGAAG8930.17259573941715597No Hit
AAGCAGTGGTATCAACGCAGAGTAC8530.1648646872596126No Hit
GTATCAACGCAGAGTACATGGGAAG8500.16428485834779682No Hit
GAGTACATGGGAAGCAGTGGTATCA7730.14940258294452582No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7670.14824292512089435No Hit
AAAAAGTACTCTGCGTTGATACCAC7380.14263791230667539No Hit
CCACTGCTTCCATGTACTCTGCGTT7300.1410917018751667No Hit
GTGGTATCAACGCAGAGTACATGGA7170.1385791099239651No Hit
ACATGGAAGCAGTGGTATCAACGCA7000.13529341275700918No Hit
GTACTCTGCGTTGATACCACTGCTT6870.13278082080580758No Hit
GCTTCCCATGTACTCTGCGTTGATA6570.12698253168765003No Hit
CTGCTTCCATGTACTCTGCGTTGAT6080.1175119927946594No Hit
GCGTTGATACCACTGCTTCCCATGT6040.11673888757890505No Hit
GAAGCAGTGGTATCAACGCAGAGTA5950.1149994008434578No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5520.10668851977409866No Hit
GTACATGGGGTGGTATCAACGCAAA5470.10572213825440573No Hit
TGGAAGCAGTGGTATCAACGCAGAG5400.10436920412683565No Hit
GTTGATACCACTGCTTCCATGTACT5340.10320954630320413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGG250.006018948319.00426110
TAGAAAT504.5616944E-617.095564
CGAACGA402.7539654E-416.62711716
CCAACGA350.002164319616.28936419
TTGCACC350.002171328816.2814854
GTGTTAC350.002176949516.2751851
TATAAGG350.002176949516.2751852
CGGTTCT651.8990977E-716.08208512
CGCATCG807.661583E-915.439465513
TCCAACG508.6793916E-515.20340818
CGCCAGT755.8034857E-815.20340818
ACGGTAT704.4129592E-714.9304729
AGGCCCG653.3537908E-614.61866110
ATCCCCG400.005261574414.25457512
AAACGCT400.00526495614.25319519
ATCGCCA801.2807504E-714.25181516
ATATCTG400.005278499314.2476785
GGATAAG400.005295469414.24078751
TTATGCT551.9604845E-413.8145934
GCATCGC903.61415E-813.7239714
Produced by FastQC (version 0.11.5)