##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062596_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517394
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09271464299934	32.0	32.0	32.0	32.0	32.0
2	30.84665264769209	32.0	32.0	32.0	32.0	32.0
3	30.815540961047095	32.0	32.0	32.0	32.0	32.0
4	30.860222576991617	32.0	32.0	32.0	32.0	32.0
5	30.718885414210447	32.0	32.0	32.0	32.0	32.0
6	34.362953571166265	36.0	36.0	36.0	32.0	36.0
7	34.20399154222894	36.0	36.0	36.0	32.0	36.0
8	34.19599376877196	36.0	36.0	36.0	32.0	36.0
9	34.3290413108772	36.0	36.0	36.0	32.0	36.0
10	34.055963153805415	36.0	36.0	36.0	32.0	36.0
11	34.350535568638215	36.0	36.0	36.0	32.0	36.0
12	34.151433916898924	36.0	36.0	36.0	32.0	36.0
13	34.232698871652936	36.0	36.0	36.0	32.0	36.0
14	34.118944170206845	36.0	36.0	36.0	32.0	36.0
15	34.08787500434872	36.0	36.0	36.0	32.0	36.0
16	34.05101334766155	36.0	36.0	36.0	32.0	36.0
17	33.97662129827559	36.0	36.0	36.0	32.0	36.0
18	33.98229782332226	36.0	36.0	36.0	32.0	36.0
19	33.96767260540323	36.0	36.0	36.0	32.0	36.0
20	33.980167918452864	36.0	36.0	36.0	32.0	36.0
21	33.872116800735995	36.0	36.0	36.0	32.0	36.0
22	33.826014603957525	36.0	36.0	36.0	32.0	36.0
23	33.666360645852095	36.0	36.0	36.0	27.0	36.0
24	33.63953389486542	36.0	36.0	36.0	27.0	36.0
25	33.05173813380132	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	21.0
5	39.0
6	100.0
7	21.0
8	88.0
9	107.0
10	59.0
11	18.0
12	43.0
13	26.0
14	102.0
15	148.0
16	220.0
17	289.0
18	419.0
19	541.0
20	850.0
21	1127.0
22	1659.0
23	2422.0
24	3258.0
25	4470.0
26	6148.0
27	7973.0
28	10718.0
29	14477.0
30	19176.0
31	26413.0
32	38407.0
33	53938.0
34	115341.0
35	208771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.27363749526438	16.2557310633297	11.650198316053164	24.820433125352757
2	14.554707477497153	21.102711938443576	37.0360992618564	27.306481322202874
3	20.07457003359324	25.782134890966933	27.480545687192432	26.66274938824739
4	12.489827477891074	16.070361958150098	35.00410766926014	36.43570289469869
5	13.32601205690488	37.6233048602706	34.256226629596256	14.794456453228259
6	34.03125568012623	33.25895187893742	18.22957958364433	14.480212857292024
7	28.654984333294646	31.11639781826622	21.773625778499863	18.454992069939266
8	28.968037483701476	32.23780174031471	21.154032167577835	17.640128608405973
9	27.04241537818638	15.777499187444862	17.736337465756606	39.44374796861216
10	15.092258420334586	27.97302396687018	32.77239697729098	24.16232063550425
11	36.8829887081636	21.721158405016013	20.243640480314276	21.15221240650611
12	22.42423421348543	24.451558087909408	29.885560228453755	23.238647470151406
13	31.538175724055716	20.067848171814948	24.483204384392984	23.91077171973635
14	24.757805756927162	19.026230130008866	24.391659925609694	31.824304187454278
15	26.25466338488212	25.734144491400794	25.605079025991113	22.40611309772597
16	26.8303679408663	25.156590137288482	25.36498998635823	22.648051935486993
17	24.173384989917675	25.198453398768443	27.837661055915287	22.79050055539859
18	26.203945445783503	22.8919463437953	29.08455051993722	21.81955769048398
19	23.970915255943037	29.469882735916798	24.595846308378025	21.963355699762143
20	23.443145466403216	23.29297061720702	30.286744940791245	22.97713897559852
21	24.63489876254582	26.92459541789722	22.723560399917943	25.71694541963902
22	22.869050637934606	27.26306599637236	24.893290410752066	24.97459295494097
23	22.139616447689317	26.052755862837852	24.50894619893002	27.29868149054281
24	22.022872913848197	28.61668998860032	24.693766560024464	24.666670537527025
25	25.673614739979485	24.716754727205867	23.623642803228243	25.98598772958641
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	99.0
2	246.0
3	393.0
4	393.0
5	393.0
6	1014.5
7	1636.0
8	1636.0
9	1636.0
10	1633.5
11	1631.0
12	1631.0
13	1631.0
14	1598.0
15	1565.0
16	1565.0
17	1565.0
18	2454.5
19	3344.0
20	3344.0
21	3344.0
22	5285.5
23	7227.0
24	7227.0
25	7227.0
26	10821.0
27	14415.0
28	14415.0
29	14415.0
30	18166.5
31	21918.0
32	21918.0
33	21918.0
34	25948.0
35	29978.0
36	29978.0
37	29978.0
38	33964.5
39	37951.0
40	37951.0
41	37951.0
42	47745.0
43	57539.0
44	57539.0
45	57539.0
46	83410.5
47	109282.0
48	109282.0
49	109282.0
50	90331.5
51	71381.0
52	71381.0
53	71381.0
54	59653.5
55	47926.0
56	47926.0
57	47926.0
58	43088.5
59	38251.0
60	38251.0
61	38251.0
62	34197.5
63	30144.0
64	30144.0
65	30144.0
66	25101.5
67	20059.0
68	20059.0
69	20059.0
70	15191.0
71	10323.0
72	10323.0
73	10323.0
74	8090.0
75	5857.0
76	5857.0
77	5857.0
78	4764.5
79	3672.0
80	3672.0
81	3672.0
82	2576.0
83	1480.0
84	1480.0
85	1480.0
86	1108.5
87	737.0
88	737.0
89	737.0
90	508.5
91	280.0
92	280.0
93	280.0
94	161.5
95	43.0
96	43.0
97	43.0
98	153.0
99	263.0
100	263.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007344499549666213
2	0.002899144559078768
3	0.005411736510280367
4	0.013336064971762332
5	0.03478973470894521
6	0.046386312945260286
7	0.07228533767303061
8	0.09199952067476623
9	0.09779780979292377
10	0.1235035582167555
11	0.12543632125614135
12	0.13587324166882492
13	0.12601615016795711
14	0.1275623605994658
15	0.11635233497102788
16	0.11577250605921212
17	0.1252430449522028
18	0.1347135838451934
19	0.1347135838451934
20	0.12814218951128153
21	0.13297409710974617
22	0.15558742467056055
23	0.1449572279539384
24	0.13780600470821078
25	0.1360665179727635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	517394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.58891710609361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23344287743244	42.939231850586395
2	10.479428103898474	10.81244695542572
3	2.649780060098116	4.100978516093444
4	1.0248861032862882	2.1149105690249446
5	0.5701631633149808	1.4707050084602329
6	0.3610330224903498	1.117518160189025
7	0.24570927120108277	0.8873112656933931
8	0.20254362925406616	0.83592051999643
9	0.13997463851426822	0.6499026020941201
>10	0.8771209413154936	8.848432810426782
>50	0.11770540176299893	4.2743923252797185
>100	0.08396818467422695	8.171876960242757
>500	0.008246875280504433	2.9336529684284884
>1k	0.0048731535748435285	5.500647662094919
>5k	7.497159345913121E-4	2.9388743679108598
>10k+	3.7485796729565604E-4	2.40319745805279
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	12427	2.4018446290447897	No Hit
TCCATGTACTCTGCGTTGATACCAC	9788	1.8917884629508652	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5409	1.0454315280038036	No Hit
CATGTACTCTGCGTTGATACCACTG	4261	0.8235503310823086	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3428	0.6625511699014678	No Hit
GCGTTGATACCACTGCTTCCATGTA	2759	0.5332493225665547	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2602	0.502904942848197	No Hit
CCCATGTACTCTGCGTTGATACCAC	2598	0.5021318376324426	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2183	0.42192217149793	No Hit
GTATCAACGCAGAGTACATGGAAGC	1930	0.3730232666014681	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1729	0.33417472950981264	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1728	0.333981453205874	No Hit
CAGTGGTATCAACGCAGAGTACATG	1568	0.3030572445757005	No Hit
TATCAACGCAGAGTACATGGAAGCA	1271	0.24565418230594094	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1270	0.24546090600200238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1117	0.2158896314993989	No Hit
ATACCACTGCTTCCATGTACTCTGC	905	0.17491505506441898	No Hit
TATCAACGCAGAGTACTTTTTTTTT	900	0.17394867354472607	No Hit
GGTATCAACGCAGAGTACATGGAAG	893	0.17259573941715597	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	853	0.1648646872596126	No Hit
GTATCAACGCAGAGTACATGGGAAG	850	0.16428485834779682	No Hit
GAGTACATGGGAAGCAGTGGTATCA	773	0.14940258294452582	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	767	0.14824292512089435	No Hit
AAAAAGTACTCTGCGTTGATACCAC	738	0.14263791230667539	No Hit
CCACTGCTTCCATGTACTCTGCGTT	730	0.1410917018751667	No Hit
GTGGTATCAACGCAGAGTACATGGA	717	0.1385791099239651	No Hit
ACATGGAAGCAGTGGTATCAACGCA	700	0.13529341275700918	No Hit
GTACTCTGCGTTGATACCACTGCTT	687	0.13278082080580758	No Hit
GCTTCCCATGTACTCTGCGTTGATA	657	0.12698253168765003	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	608	0.1175119927946594	No Hit
GCGTTGATACCACTGCTTCCCATGT	604	0.11673888757890505	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	595	0.1149994008434578	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	552	0.10668851977409866	No Hit
GTACATGGGGTGGTATCAACGCAAA	547	0.10572213825440573	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	540	0.10436920412683565	No Hit
GTTGATACCACTGCTTCCATGTACT	534	0.10320954630320413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.9327630393858452E-4	0.0
8	0.0	0.0	0.0	1.9327630393858452E-4	0.0
9	0.0	0.0	0.0	1.9327630393858452E-4	0.0
10	0.0	0.0	0.0	1.9327630393858452E-4	0.0
11	0.0	0.0	0.0	3.8655260787716903E-4	0.0
12	0.0	0.0	0.0	3.8655260787716903E-4	1.9327630393858452E-4
13	0.0	0.0	0.0	3.8655260787716903E-4	1.9327630393858452E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGG	25	0.0060189483	19.004261	10
TAGAAAT	50	4.5616944E-6	17.09556	4
CGAACGA	40	2.7539654E-4	16.627117	16
CCAACGA	35	0.0021643196	16.289364	19
TTGCACC	35	0.0021713288	16.281485	4
GTGTTAC	35	0.0021769495	16.275185	1
TATAAGG	35	0.0021769495	16.275185	2
CGGTTCT	65	1.8990977E-7	16.082085	12
CGCATCG	80	7.661583E-9	15.4394655	13
TCCAACG	50	8.6793916E-5	15.203408	18
CGCCAGT	75	5.8034857E-8	15.203408	18
ACGGTAT	70	4.4129592E-7	14.930472	9
AGGCCCG	65	3.3537908E-6	14.618661	10
ATCCCCG	40	0.0052615744	14.254575	12
AAACGCT	40	0.005264956	14.253195	19
ATCGCCA	80	1.2807504E-7	14.251815	16
ATATCTG	40	0.0052784993	14.247678	5
GGATAAG	40	0.0052954694	14.2407875	1
TTATGCT	55	1.9604845E-4	13.814593	4
GCATCGC	90	3.61415E-8	13.72397	14
>>END_MODULE