FastQCFastQC Report
Thu 2 Feb 2017
SRR4062596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517394
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48450.9364236925824421No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43280.8364998434461939No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20000.38655260787716905No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18590.3593006490218286No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12260.2369567486287046No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG11210.21666273671515326No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC11090.21434342106789023No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10160.19636872480160186No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8830.17066297637777014No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA8070.1559739772784377No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7900.15268828011148178No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7790.15056224076815736No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC6290.12157079517736967No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG6080.1175119927946594No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA5640.10900783542136168No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5470.10572213825440573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1051.10958354E-1025.1523591
GTATTAC551.5878252E-424.009071
ACTTTGC551.5919958E-423.9997888
TATCCGA551.5919958E-423.99978828
CGAGCCG1750.023.88550215
AGGCCCG1950.023.692110
TATATTG651.8109151E-523.6920995
ACCCGCC651.8109151E-523.69209915
GCATTCG1450.022.7584222
GTCCAGG1800.022.0083141
GCTTTAA602.8623774E-422.0083141
CTATACA602.869874E-421.9998074
TAACGGC602.869874E-421.99980736
GCCGCCT1900.021.99980718
TTAACGG602.869874E-421.99980735
CGCGTAT703.2109885E-521.99980744
TAGGACG1205.966285E-1021.9998074
ATACGCG803.6092024E-621.99980741
ACGCGTA703.2109885E-521.99980743
CCGAGCC2000.021.99980514
Produced by FastQC (version 0.11.5)