FastQCFastQC Report
Thu 2 Feb 2017
SRR4062595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23755
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1030.4335929278046727No Hit
TATCAACGCAGAGTACTTTTTTTTT1000.4209640075773522No Hit
GGTATCAACGCAGAGTACTTTTTTT430.18101452325826142No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG400.16838560303094086No Hit
GTACATGGAAGCAGTGGTATCAACG340.14312776257629972No Hit
CTCCGAGGTCACCCCAACCGAAATT340.14312776257629972No Hit
CTCCCGACCCGGGGAGGTAGTGACG340.14312776257629972No Hit
GAATAACGCCGCCGCATCGCCAGTC320.1347084824247527No Hit
GGCTCCACTCCTGGTGGTGCCCTTC310.13049884234897915No Hit
CTGTAGGACGTGGAATATGGCAAGA310.13049884234897915No Hit
TCGTAGTTCCGACCATAAACGATGC300.12628920227320564No Hit
ACGCAGAGTACTTTTTTTTTTTTTT300.12628920227320564No Hit
GTATCTGATCGTCTTCGAACCTCCG290.12207956219743213No Hit
GCGCAAGACGGACCAGAGCGAAAGC290.12207956219743213No Hit
GAATAATTGCAATCCCCGATCCCCA290.12207956219743213No Hit
GGACTCTTTCGAGGCCCTGTAATTG280.11786992212165859No Hit
CTCTTAATCATGGCCTCAGTTCCGA280.11786992212165859No Hit
GATTAAGAGGGACGGCCGGGGGCAT280.11786992212165859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG270.11366028204588509No Hit
GAACTACGACGGTATCTGATCGTCT270.11366028204588509No Hit
GAGTACTTTTTTTTTTTTTTTTTTT260.10945064197011155No Hit
ATATTAAAGTTGCTGCAGTTAAAAA260.10945064197011155No Hit
TCCATGTACTCTGCGTTGATACCAC250.10524100189433805No Hit
GTACTTTTTTTTTTTTTTTTTTTTT250.10524100189433805No Hit
GTCCTAAAGTGTGTATTTCTCATTT250.10524100189433805No Hit
CTCCACTCCTGGTGGTGCCCTTCCG250.10524100189433805No Hit
GTATCAACGCAGAGTACATGGGCAG240.10103136181856451No Hit
GTCCTACAGTGTGCATTTCTCATTT240.10103136181856451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)