##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062595_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23755
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.6862555251526	32.0	32.0	32.0	14.0	32.0
2	27.29017049042307	32.0	21.0	32.0	14.0	32.0
3	27.567417385813513	32.0	21.0	32.0	14.0	32.0
4	27.06297621553357	32.0	21.0	32.0	14.0	32.0
5	27.346495474636917	32.0	21.0	32.0	14.0	32.0
6	30.707135339928435	36.0	21.0	36.0	14.0	36.0
7	30.571290254683223	36.0	21.0	36.0	14.0	36.0
8	30.260366238686593	36.0	21.0	36.0	14.0	36.0
9	30.58678173016207	36.0	27.0	36.0	14.0	36.0
10	29.516480740896654	36.0	21.0	36.0	14.0	36.0
11	31.176931172384762	36.0	32.0	36.0	14.0	36.0
12	30.204209640075774	36.0	21.0	36.0	14.0	36.0
13	30.669964218059356	36.0	27.0	36.0	14.0	36.0
14	30.147505788255103	36.0	21.0	36.0	14.0	36.0
15	29.96750157861503	36.0	21.0	36.0	14.0	36.0
16	30.213344559040202	36.0	21.0	36.0	14.0	36.0
17	29.908271942748893	36.0	21.0	36.0	14.0	36.0
18	30.065544095979792	36.0	21.0	36.0	14.0	36.0
19	30.021427067985687	36.0	21.0	36.0	14.0	36.0
20	29.97027994106504	36.0	21.0	36.0	14.0	36.0
21	29.987834140181015	36.0	21.0	36.0	14.0	36.0
22	29.7189223321406	36.0	21.0	36.0	14.0	36.0
23	29.675394653757103	36.0	21.0	36.0	14.0	36.0
24	29.77394232793096	36.0	21.0	36.0	14.0	36.0
25	29.03704483266681	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	2.0
6	2.0
7	2.0
8	4.0
9	0.0
10	1.0
11	2.0
12	1.0
13	2.0
14	18.0
15	75.0
16	176.0
17	420.0
18	557.0
19	703.0
20	784.0
21	793.0
22	756.0
23	688.0
24	708.0
25	690.0
26	775.0
27	816.0
28	836.0
29	999.0
30	1134.0
31	1266.0
32	1644.0
33	2114.0
34	3477.0
35	4309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37234266470217	20.72826773310882	12.245842980425174	25.65354662176384
2	15.760892443696065	20.829299094927382	39.66322879393812	23.746579667438432
3	17.583666596506	25.472532098505578	29.307514207535256	27.636287097453167
4	11.640634867174672	16.414768660800743	37.906790721172065	34.03780575085252
5	13.675213675213676	37.345795966485625	34.086985811123746	14.89200454717696
6	31.284210526315793	37.74315789473684	17.246315789473684	13.726315789473684
7	27.480626684636118	32.34501347708895	21.702324797843666	18.472035040431265
8	25.991913065453627	33.75031589588072	20.053070507960577	20.20470053070508
9	26.500695058764055	14.545684316946797	20.047179746408865	38.906440877880286
10	15.43841907891965	27.20262925041082	31.580499726119747	25.778451944549786
11	35.49188961449337	21.95070570886876	23.720244364862015	18.837160311775857
12	24.349951536095073	25.669012600615282	29.23005604955961	20.750979813730037
13	28.472719612386772	20.800505582473143	25.843690752053927	24.88308405308616
14	22.3519002275217	21.648268307070026	25.785792533917583	30.214038931490688
15	23.334878038505288	29.36765387369929	23.124236424147952	24.17323166364747
16	23.970005897716742	26.69138090824838	25.958378970427166	23.38023422360772
17	22.353783920444968	27.29226361031519	26.790830945558742	23.563121523681104
18	22.719035779004592	25.15908803573686	29.466054195288464	22.65582198997008
19	24.425904858214302	25.774238402224753	27.3163948931867	22.483461846374247
20	24.305760397791918	25.16118157684042	27.162782857865242	23.37027516750242
21	25.26652901268383	24.85356706417766	25.93232480721419	23.94757911592432
22	24.165542818610923	26.96813890761969	26.099966284558327	22.76635198921106
23	22.0751854349292	26.20954146999326	27.469656102494945	24.2456169925826
24	23.594605983986515	26.409608091024023	27.265065318162662	22.7307206068268
25	22.802730260385946	25.46557680964018	27.589112665374564	24.14258026459931
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	10.0
3	19.0
4	19.0
5	19.0
6	42.5
7	66.0
8	66.0
9	66.0
10	83.0
11	100.0
12	100.0
13	100.0
14	125.0
15	150.0
16	150.0
17	150.0
18	219.5
19	289.0
20	289.0
21	289.0
22	475.5
23	662.0
24	662.0
25	662.0
26	935.5
27	1209.0
28	1209.0
29	1209.0
30	1430.5
31	1652.0
32	1652.0
33	1652.0
34	1835.0
35	2018.0
36	2018.0
37	2018.0
38	2075.0
39	2132.0
40	2132.0
41	2132.0
42	2258.5
43	2385.0
44	2385.0
45	2385.0
46	2676.0
47	2967.0
48	2967.0
49	2967.0
50	2799.0
51	2631.0
52	2631.0
53	2631.0
54	2350.0
55	2069.0
56	2069.0
57	2069.0
58	1874.5
59	1680.0
60	1680.0
61	1680.0
62	1517.0
63	1354.0
64	1354.0
65	1354.0
66	1182.0
67	1010.0
68	1010.0
69	1010.0
70	782.0
71	554.0
72	554.0
73	554.0
74	444.5
75	335.0
76	335.0
77	335.0
78	293.0
79	251.0
80	251.0
81	251.0
82	182.5
83	114.0
84	114.0
85	114.0
86	86.5
87	59.0
88	59.0
89	59.0
90	41.0
91	23.0
92	23.0
93	23.0
94	14.5
95	6.0
96	6.0
97	6.0
98	12.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.008419280151547043
5	0.016838560303094087
6	0.021048200378867607
7	0.046306040833508734
8	0.054725320985055774
9	0.06735424121237635
10	0.09261208166701747
11	0.08419280151547043
12	0.10945064197011155
13	0.08419280151547043
14	0.08840244159124394
15	0.07577352136392339
16	0.07156388128814986
17	0.096821721742791
18	0.10945064197011155
19	0.09261208166701747
20	0.10103136181856451
21	0.10103136181856451
22	0.11366028204588509
23	0.11366028204588509
24	0.10524100189433805
25	0.08840244159124394
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	23755.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.38434013891812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4753820033956	67.29951589139128
2	4.4142614601018675	6.567038518206693
3	1.561969439728353	3.485581982740476
4	0.89417091114884	2.6604925278888656
5	0.588568194680249	2.189012839402231
6	0.4244482173174873	1.8943380340980847
7	0.3508771929824561	1.8269837928857082
8	0.2546689303904924	1.5154704272784676
9	0.14714204867006225	0.9850557777310041
>10	0.8771929824561403	10.72195327299516
>50	0.005659309564233164	0.4209640075773522
>100	0.005659309564233164	0.4335929278046727
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	103	0.4335929278046727	No Hit
TATCAACGCAGAGTACTTTTTTTTT	100	0.4209640075773522	No Hit
GGTATCAACGCAGAGTACTTTTTTT	43	0.18101452325826142	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	40	0.16838560303094086	No Hit
GTACATGGAAGCAGTGGTATCAACG	34	0.14312776257629972	No Hit
CTCCGAGGTCACCCCAACCGAAATT	34	0.14312776257629972	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	34	0.14312776257629972	No Hit
GAATAACGCCGCCGCATCGCCAGTC	32	0.1347084824247527	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	31	0.13049884234897915	No Hit
CTGTAGGACGTGGAATATGGCAAGA	31	0.13049884234897915	No Hit
TCGTAGTTCCGACCATAAACGATGC	30	0.12628920227320564	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	30	0.12628920227320564	No Hit
GTATCTGATCGTCTTCGAACCTCCG	29	0.12207956219743213	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	29	0.12207956219743213	No Hit
GAATAATTGCAATCCCCGATCCCCA	29	0.12207956219743213	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	28	0.11786992212165859	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	28	0.11786992212165859	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	28	0.11786992212165859	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	27	0.11366028204588509	No Hit
GAACTACGACGGTATCTGATCGTCT	27	0.11366028204588509	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	26	0.10945064197011155	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	26	0.10945064197011155	No Hit
TCCATGTACTCTGCGTTGATACCAC	25	0.10524100189433805	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	25	0.10524100189433805	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	25	0.10524100189433805	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	25	0.10524100189433805	No Hit
GTATCAACGCAGAGTACATGGGCAG	24	0.10103136181856451	No Hit
GTCCTACAGTGTGCATTTCTCATTT	24	0.10103136181856451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE