##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062595_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23755
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.932982529993687	32.0	32.0	32.0	21.0	32.0
2	29.868785518838138	32.0	32.0	32.0	21.0	32.0
3	30.736308145653545	32.0	32.0	32.0	32.0	32.0
4	31.198189854767417	32.0	32.0	32.0	32.0	32.0
5	30.93121448116186	32.0	32.0	32.0	32.0	32.0
6	34.46903809724269	36.0	36.0	36.0	32.0	36.0
7	34.44714796884866	36.0	36.0	36.0	32.0	36.0
8	34.31328141443907	36.0	36.0	36.0	32.0	36.0
9	34.67775205219954	36.0	36.0	36.0	32.0	36.0
10	34.337402652073244	36.0	36.0	36.0	32.0	36.0
11	34.809387497368974	36.0	36.0	36.0	32.0	36.0
12	34.547337402652076	36.0	36.0	36.0	32.0	36.0
13	34.67863607661545	36.0	36.0	36.0	32.0	36.0
14	34.58236160808251	36.0	36.0	36.0	32.0	36.0
15	34.523510839823196	36.0	36.0	36.0	32.0	36.0
16	34.553020416754364	36.0	36.0	36.0	32.0	36.0
17	34.54455904020206	36.0	36.0	36.0	32.0	36.0
18	34.54220164175963	36.0	36.0	36.0	32.0	36.0
19	34.48848663439276	36.0	36.0	36.0	32.0	36.0
20	34.54135971374448	36.0	36.0	36.0	32.0	36.0
21	34.57882551041886	36.0	36.0	36.0	32.0	36.0
22	34.509997895179964	36.0	36.0	36.0	32.0	36.0
23	34.493454009682175	36.0	36.0	36.0	32.0	36.0
24	34.41427067985687	36.0	36.0	36.0	32.0	36.0
25	34.41086087139549	36.0	36.0	36.0	32.0	36.0
26	34.3531888023574	36.0	36.0	36.0	32.0	36.0
27	34.3542833087771	36.0	36.0	36.0	32.0	36.0
28	34.24495895600926	36.0	36.0	36.0	32.0	36.0
29	34.12064828457167	36.0	36.0	36.0	32.0	36.0
30	34.16649126499684	36.0	36.0	36.0	32.0	36.0
31	34.21355504104399	36.0	36.0	36.0	32.0	36.0
32	34.12847821511261	36.0	36.0	36.0	32.0	36.0
33	34.099894758998104	36.0	36.0	36.0	32.0	36.0
34	34.01481793306672	36.0	36.0	36.0	32.0	36.0
35	33.966954325405176	36.0	36.0	36.0	32.0	36.0
36	33.88756051357609	36.0	36.0	36.0	32.0	36.0
37	33.94228583456115	36.0	36.0	36.0	32.0	36.0
38	33.90166280782993	36.0	36.0	36.0	32.0	36.0
39	33.74194906335509	36.0	36.0	36.0	32.0	36.0
40	33.81363923384551	36.0	36.0	36.0	32.0	36.0
41	33.790023153020414	36.0	36.0	36.0	32.0	36.0
42	33.6911387076405	36.0	36.0	36.0	27.0	36.0
43	33.7545779835824	36.0	36.0	36.0	32.0	36.0
44	33.7167333193012	36.0	36.0	36.0	27.0	36.0
45	33.58636076615449	36.0	36.0	36.0	21.0	36.0
46	33.530625131551254	36.0	36.0	36.0	21.0	36.0
47	33.612123763418225	36.0	36.0	36.0	21.0	36.0
48	33.51323931803831	36.0	36.0	36.0	21.0	36.0
49	33.46920648284571	36.0	36.0	36.0	21.0	36.0
50	32.86781730162071	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	0.0
22	3.0
23	15.0
24	28.0
25	88.0
26	178.0
27	249.0
28	372.0
29	616.0
30	910.0
31	1458.0
32	2044.0
33	3265.0
34	5623.0
35	8904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.443906545990316	20.85034729530625	12.89833719217007	24.807408966533362
2	17.47495158710112	22.147848783362804	37.02534310010945	23.351856529426623
3	20.244159124394866	24.622184803199325	29.741107135339927	25.39254893706588
4	13.685539886339718	17.68048831824879	38.03830772468954	30.595664070721952
5	16.5817722584719	35.062092191117664	33.45821932224794	14.897916228162492
6	32.62050094716902	38.06777520521996	16.56914333824458	12.74258050936645
7	28.579246474426434	31.82066933277205	21.010313618185645	18.589770574615873
8	26.31446011366028	34.58219322247948	19.579035992422646	19.524310671437593
9	26.592296358661333	14.001262892022732	19.02757314249632	40.37886760681962
10	15.861923805514627	26.756472321616503	31.433382445800884	25.948221427067985
11	36.26183961271312	21.39760050515681	23.52767838349821	18.812881498631867
12	23.881288149863185	25.337823616080822	29.130709324352765	21.65017890970322
13	28.756051357608925	20.53883392969901	25.598821300778784	25.106293411913285
14	22.037465796674386	21.906966954325405	25.44727425805094	30.608292990949277
15	24.272784676910124	29.286466007156385	22.167964639023364	24.272784676910124
16	24.142285834561143	27.661544937907813	24.93369816880657	23.26247105872448
17	22.828878130919804	27.825720900862976	25.148389812671017	24.1970111555462
18	23.90654599031783	25.40096821721743	27.707850978741316	22.984634813723424
19	24.382235318880234	26.93327720479899	25.29993685539886	23.384550620921914
20	24.655861923805514	25.910334666386024	25.624079141233423	23.809724268575035
21	26.33971795411492	25.661965901915384	24.445379920016837	23.55293622395285
22	24.596926962744686	27.396337613134076	24.630604083350875	23.376131340770364
23	23.068827615238895	26.45337823616081	26.15870343085666	24.319090717743634
24	23.679225426226058	27.358450852452115	25.55672490002105	23.40559882130078
25	23.540307303725534	26.94590612502631	25.986108187749945	23.52767838349821
26	22.84150705114713	27.74994737949905	26.23026731214481	23.17827825720901
27	23.999158071984844	27.131130288360346	25.379920016838557	23.48979162281625
28	22.155335718796042	27.392127973058304	26.89539044411703	23.557145864028627
29	23.26247105872448	27.017470006314458	25.969269627446856	23.750789307514207
30	23.144601136602823	26.828036202904652	26.57124815828247	23.45611450221006
31	24.04125447274258	26.844874763207745	25.37571037676279	23.738160387286886
32	22.900442012207954	27.585771416543885	25.586192380551466	23.927594190696695
33	22.597347926752263	26.567038518206694	26.14607451062934	24.689539044411703
34	23.098295095769313	26.638602399494843	27.11429172805725	23.148810776678594
35	24.655861923805514	26.364975794569563	26.30183119343296	22.67733108819196
36	22.407914123342454	27.274258050936645	26.30604083350874	24.011786992212166
37	24.47063776047148	26.781730162071142	24.7695222058514	23.978109871605977
38	23.030940854556935	26.638602399494843	26.162913070932436	24.167543675015786
39	23.91917491054515	26.238686592296357	25.287307935171544	24.55483056198695
40	24.651652283729742	26.80277836245001	25.71669122290044	22.828878130919804
41	23.24142285834561	26.33971795411492	27.046937486844875	23.37192170069459
42	23.973900231530205	27.49736897495264	26.238686592296357	22.290044201220795
43	22.854135971374447	26.297621553357185	26.815407282677334	24.032835192591033
44	22.938328772889918	26.94169648495054	26.293411913281417	23.82656282887813
45	23.051989054935802	27.02588928646601	26.171332351083983	23.750789307514207
46	22.483687644706375	26.87434224373816	26.87434224373816	23.7676278678173
47	23.258261418648708	25.95664070721953	26.76489160176805	24.020206272363712
48	23.472953062513156	27.35424121237634	25.78825510418859	23.384550620921914
49	22.041675436750158	27.29530625131551	26.903809724268573	23.759208587665753
50	22.60997684697958	27.45948221427068	25.994527467901495	23.93601347084824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	6.0
4	5.0
5	10.0
6	15.0
7	19.5
8	24.0
9	31.0
10	38.0
11	32.0
12	26.0
13	38.5
14	51.0
15	67.0
16	83.0
17	95.0
18	107.0
19	117.5
20	128.0
21	147.5
22	167.0
23	202.0
24	237.0
25	291.0
26	345.0
27	409.5
28	474.0
29	587.5
30	701.0
31	710.0
32	719.0
33	801.5
34	884.0
35	1020.5
36	1157.0
37	1225.0
38	1293.0
39	1275.5
40	1258.0
41	1314.0
42	1370.0
43	1303.5
44	1237.0
45	1277.0
46	1317.0
47	1346.0
48	1375.0
49	1381.0
50	1387.0
51	1296.0
52	1205.0
53	1132.0
54	1059.0
55	1156.0
56	1253.0
57	1235.5
58	1218.0
59	1087.0
60	956.0
61	828.0
62	700.0
63	672.0
64	644.0
65	585.0
66	526.0
67	466.0
68	406.0
69	392.5
70	379.0
71	308.5
72	238.0
73	230.5
74	223.0
75	184.5
76	146.0
77	121.5
78	97.0
79	101.5
80	106.0
81	80.0
82	54.0
83	50.0
84	46.0
85	41.0
86	36.0
87	26.0
88	16.0
89	13.0
90	10.0
91	7.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	3.0
98	5.0
99	11.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004209640075773522
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	23755.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.84613765523048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.27805259047123	74.60324142285835
2	3.775058578495184	6.103978109871606
3	1.3329862015100236	3.2330035781940643
4	0.7654256703983338	2.4752683645548306
5	0.47904191616766467	1.9364344348558198
6	0.3436605050768029	1.6670174700063145
7	0.15620932048945588	0.8840244159124395
8	0.1301744337412132	0.8419280151547044
9	0.15100234313980734	1.098716059776889
>10	0.5883884405102837	7.156388128814986
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	43	0.18101452325826142	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	41	0.17259524310671437	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	36	0.15154704272784678	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	35	0.14733740265207323	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	32	0.1347084824247527	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	29	0.12207956219743213	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	28	0.11786992212165859	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	26	0.10945064197011155	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	24	0.10103136181856451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.004209640075773522	0.0
4	0.0	0.0	0.0	0.004209640075773522	0.0
5	0.0	0.0	0.0	0.004209640075773522	0.0
6	0.0	0.0	0.0	0.004209640075773522	0.0
7	0.0	0.0	0.0	0.004209640075773522	0.0
8	0.0	0.0	0.0	0.004209640075773522	0.0
9	0.0	0.0	0.0	0.004209640075773522	0.0
10	0.0	0.0	0.0	0.004209640075773522	0.0
11	0.0	0.0	0.0	0.004209640075773522	0.0
12	0.0	0.0	0.0	0.004209640075773522	0.0
13	0.0	0.0	0.0	0.004209640075773522	0.0
14	0.0	0.0	0.0	0.004209640075773522	0.0
15	0.0	0.0	0.0	0.004209640075773522	0.0
16	0.0	0.0	0.0	0.004209640075773522	0.0
17	0.0	0.0	0.0	0.004209640075773522	0.0
18	0.0	0.0	0.0	0.004209640075773522	0.0
19	0.0	0.0	0.0	0.004209640075773522	0.0
20	0.0	0.0	0.0	0.004209640075773522	0.0
21	0.0	0.0	0.0	0.004209640075773522	0.0
22	0.0	0.0	0.0	0.008419280151547043	0.0
23	0.0	0.0	0.0	0.012628920227320563	0.0
24	0.0	0.0	0.0	0.012628920227320563	0.0
25	0.0	0.0	0.0	0.012628920227320563	0.0
26	0.0	0.0	0.0	0.016838560303094087	0.0
27	0.0	0.0	0.0	0.016838560303094087	0.0
28	0.0	0.0	0.0	0.016838560303094087	0.0
29	0.0	0.0	0.0	0.025257840454641127	0.0
30	0.0	0.0	0.0	0.0589349610608293	0.0
31	0.0	0.0	0.0	0.14733740265207323	0.0
32	0.0	0.0	0.0	0.22732056409177015	0.0
33	0.0	0.0	0.0	0.3283519259103347	0.0
34	0.0	0.0	0.0	0.42517364765312565	0.0
35	0.0	0.0	0.0	0.5346242896232372	0.0
36	0.0	0.0	0.0	0.6567038518206694	0.0
37	0.0	0.0	0.0	0.8503472953062513	0.0
38	0.0	0.0	0.0	1.1450221006103978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	95	0.0060093887	13.893407	3
>>END_MODULE