FastQCFastQC Report
Thu 2 Feb 2017
SRR4062594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798801
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT117020.6505444459948599No Hit
GTCCTACAGTGGACATTTCTAAATT114480.6364239290505175No Hit
CTGTAGGACGTGGAATATGGCAAGA107430.5972311556420082No Hit
CTTTAGGACGTGAAATATGGCGAGG95490.53085360748632No Hit
GTCCTACAGTGTGCATTTCTCATTT76140.42328195281190084No Hit
CTGTAGGACCTGGAATATGGCGAGA57990.32238140850488745No Hit
CTGAAGGACCTGGAATATGGCGAGA51820.2880807826991424No Hit
GTCCTTCAGTGTGCATTTCTCATTT47080.26172989674788927No Hit
ATTTAGAAATGTCCACTGTAGGACG43100.23960404736265992No Hit
TTTCTAAATTTTCCACCTTTTTCAG34620.1924615340996586No Hit
GAATATGGCAAGAAAACTGAAAATC33380.18556805338667257No Hit
GTATCAACGCAGAGTACTTTTTTTT32480.18056472061111817No Hit
GGAATATGGCGAGAAAACTGAAAAT29710.1651655741796897No Hit
GATATACACTGTTCTACAAATCCCG28180.15665990846124725No Hit
TTGTAGAACAGTGTATATCAATGAG27210.15126742758092754No Hit
ATTCCAGGTCCTTCAGTGTGCATTT25070.13937061409238707No Hit
CTGTAGGACATGGAATATGGCAAGA24550.13647979959984458No Hit
GTGTATATCAATGAGTTACAATGAA23970.13325542958893172No Hit
GCCATATTCCACGTCCTACAGTGGA23540.13086494837394463No Hit
ACCTGGAATATGGCGAGAAAACTGA23240.1291971707820932No Hit
GAAATATGGCGAGGAAAACTGAAAA22380.124416208352119No Hit
GACCTGGAATATGGCGAGAAAACTG22380.124416208352119No Hit
CCATATTCCAGGTCCTTCAGTGTGC21940.12197013455073685No Hit
CCATATTTCACGTCCTAAAGTGTGT21600.1200799866133052No Hit
TTCCAGGTCCTTCAGTGTGCATTTC21280.11830102384866364No Hit
TATCAACGCAGAGTACTTTTTTTTT20910.11624409815204685No Hit
ACAGTGGACATTTCTAAATTTTCCA20410.1134644688322944No Hit
CACTTTAGGACGTGAAATATGGCGA19810.1101289136485915No Hit
GTCCACTGTAGGACGTGGAATATGG19720.10962858037103604No Hit
ATACACACTTTAGGACGTGAAATAT19500.10840554347034496No Hit
CCTAAAGTGTGTATTTCTCATTTTC18690.10390254397234602No Hit
GTGTATATCAATGAGTTACAATGAG18520.1029574700036302No Hit
CCACTGTAGGACGTGGAATATGGCA18470.10267950707165494No Hit
GTTCTACAGTGTGGTTTTTATCATT18260.10151206275735893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCT350.0021639716.29292319
TTCGTGA350.002166386516.29020116
TCGCGTA551.1285349E-515.545419
TTCGTTC456.73613E-414.78555518
CCGTCGT653.3729357E-614.6153419
CGCGTAA602.568098E-514.25035410
TCGGCGT903.6361598E-813.72332813
ATACGCT903.641435E-813.7218018
TAGGACC22500.013.5506534
GCGTTAT852.7247006E-713.4016531
CGCCACT655.430814E-513.15966917
GTCGAGA801.9899835E-613.06828219
GCGTAAC802.0012121E-613.06173411
CGGCGTC957.376002E-813.00177214
TACGCTA957.3894626E-812.9999619
CGGTCCA1051.9934305E-812.66698210
GGGTCGC751.4793541E-512.6662776
GTCGTAG751.4797775E-512.66592511
TAGAAAT13550.012.4772374
AAATGTC12900.012.2980717
Produced by FastQC (version 0.11.5)