##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062594_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1798801
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244621834210676	32.0	32.0	32.0	32.0	32.0
2	30.973429523332488	32.0	32.0	32.0	32.0	32.0
3	30.944684820611062	32.0	32.0	32.0	32.0	32.0
4	31.00667833740364	32.0	32.0	32.0	32.0	32.0
5	30.907879192862357	32.0	32.0	32.0	32.0	32.0
6	34.565859147287554	36.0	36.0	36.0	32.0	36.0
7	34.43606657990517	36.0	36.0	36.0	32.0	36.0
8	34.462305168831904	36.0	36.0	36.0	32.0	36.0
9	34.54420194340563	36.0	36.0	36.0	32.0	36.0
10	34.275679188526134	36.0	36.0	36.0	32.0	36.0
11	34.53911410989876	36.0	36.0	36.0	32.0	36.0
12	34.35108886419343	36.0	36.0	36.0	32.0	36.0
13	34.433192443188545	36.0	36.0	36.0	32.0	36.0
14	34.37284446695327	36.0	36.0	36.0	32.0	36.0
15	34.34136572083293	36.0	36.0	36.0	32.0	36.0
16	34.35954116102893	36.0	36.0	36.0	32.0	36.0
17	34.32358498800034	36.0	36.0	36.0	32.0	36.0
18	34.34720349833027	36.0	36.0	36.0	32.0	36.0
19	34.269103141481466	36.0	36.0	36.0	32.0	36.0
20	34.26651363880718	36.0	36.0	36.0	32.0	36.0
21	34.217977419403255	36.0	36.0	36.0	32.0	36.0
22	34.18184279417234	36.0	36.0	36.0	32.0	36.0
23	34.178979775973	36.0	36.0	36.0	32.0	36.0
24	34.13521951566627	36.0	36.0	36.0	32.0	36.0
25	33.736433880123485	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	62.0
5	188.0
6	300.0
7	74.0
8	288.0
9	342.0
10	216.0
11	59.0
12	140.0
13	104.0
14	331.0
15	377.0
16	568.0
17	720.0
18	879.0
19	1227.0
20	1895.0
21	2697.0
22	4211.0
23	6078.0
24	8776.0
25	12832.0
26	17874.0
27	23752.0
28	32287.0
29	43975.0
30	56755.0
31	77947.0
32	111599.0
33	163642.0
34	385380.0
35	843212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38363542050635	20.906255959320667	12.454820357233997	25.25528826293899
2	11.987600222822396	21.674703765187004	46.66794904438769	19.669746967602915
3	18.40206990832961	27.411886295140953	30.355579251326077	23.83046454520336
4	10.445617634548503	17.830988343160886	40.89129087052374	30.832103151766876
5	10.430814285547447	40.66511581606237	36.96137091782513	11.942698980565057
6	28.435352583087912	39.90906284675004	19.865958079919018	11.789626490243027
7	25.800888534829873	32.61276637408585	23.99149739147269	17.594847699611584
8	22.10645101876495	42.61507394858571	21.330935431942205	13.94753960070713
9	26.465763243065172	15.409156067614314	21.973760945923686	36.15131974339683
10	14.367996508969144	30.234987788071273	36.104617378120054	19.292398324839528
11	32.82334612244034	21.569136042293668	28.77198411872329	16.8355337165427
12	24.486668266203804	24.656122710848035	34.64168790877962	16.215521114168542
13	29.419721056882373	23.88477579739581	27.024374821534625	19.671128324187187
14	18.411728982965275	25.776966079174755	28.418536638042603	27.392768299817366
15	19.55969630466157	38.68130412446589	23.259877027989017	18.499122542883526
16	18.056998019696174	29.127553161528898	34.23871844621288	18.57673037256205
17	18.181453834978207	32.28345273282901	31.025221403959947	18.50987202823283
18	18.400757520090313	27.98677123205009	36.96378510655827	16.648686141301322
19	22.97324303937696	26.864957093670238	28.591787258086516	21.57001260886629
20	23.123949369343293	29.259799392164936	30.525398822180417	17.090852416311353
21	20.692774488120772	28.012587600222894	27.536739348386273	23.75789856327006
22	21.962321258155505	32.78777701436393	28.21941330470717	17.03048842277339
23	18.194000377460803	32.18989061987637	30.745240124640006	18.87086887802282
24	22.40962165183418	28.094920508027137	31.940697507406966	17.55476033273172
25	19.829055642087894	30.56373561407591	31.51476251604621	18.09244622778998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	314.0
1	314.0
2	714.0
3	1114.0
4	1114.0
5	1114.0
6	2440.0
7	3766.0
8	3766.0
9	3766.0
10	4638.0
11	5510.0
12	5510.0
13	5510.0
14	7932.0
15	10354.0
16	10354.0
17	10354.0
18	17941.0
19	25528.0
20	25528.0
21	25528.0
22	44409.5
23	63291.0
24	63291.0
25	63291.0
26	98127.5
27	132964.0
28	132964.0
29	132964.0
30	178886.5
31	224809.0
32	224809.0
33	224809.0
34	240336.0
35	255863.0
36	255863.0
37	255863.0
38	254604.0
39	253345.0
40	253345.0
41	253345.0
42	247840.5
43	242336.0
44	242336.0
45	242336.0
46	232975.0
47	223614.0
48	223614.0
49	223614.0
50	190594.0
51	157574.0
52	157574.0
53	157574.0
54	118280.5
55	78987.0
56	78987.0
57	78987.0
58	63496.5
59	48006.0
60	48006.0
61	48006.0
62	39261.5
63	30517.0
64	30517.0
65	30517.0
66	24479.0
67	18441.0
68	18441.0
69	18441.0
70	13678.0
71	8915.0
72	8915.0
73	8915.0
74	7214.0
75	5513.0
76	5513.0
77	5513.0
78	4895.5
79	4278.0
80	4278.0
81	4278.0
82	3008.5
83	1739.0
84	1739.0
85	1739.0
86	1352.5
87	966.0
88	966.0
89	966.0
90	653.0
91	340.0
92	340.0
93	340.0
94	223.5
95	107.0
96	107.0
97	107.0
98	358.5
99	610.0
100	610.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008227702786467207
2	0.0032799625973078733
3	0.004113851393233604
4	0.015899479708983928
5	0.03602399598399156
6	0.04758725395416169
7	0.06865684419788515
8	0.09306198962531152
9	0.09923276671516194
10	0.12213691230992199
11	0.12341554179700812
12	0.1360906514950792
13	0.1241938380065388
14	0.12752939319024173
15	0.11852339419424383
16	0.11713357953436762
17	0.12847446715895755
18	0.1341449109712525
19	0.13597946632228913
20	0.1256948378392051
21	0.13508998493996835
22	0.15426942724626017
23	0.14387361359038603
24	0.14003772512912768
25	0.13492320718078318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1798801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.460525406833824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.91790132654586	44.8629509595598
2	10.669412300249055	11.407847747068999
3	2.3470604322563595	3.7642525161004645
4	0.8312595379879587	1.7775828660111286
5	0.44176154561375086	1.1808402166523055
6	0.2936342221330695	0.9418703875596509
7	0.20555703704205242	0.7692431040938088
8	0.13982246787904337	0.5979986077195044
9	0.11591585277381546	0.5577230153042434
>10	0.8373753577264511	8.784091401117141
>50	0.10352960251159533	3.8814375879842955
>100	0.0811289370523822	8.738388623089786
>500	0.008759172928074817	3.1549132900705055
>1k	0.0061522762232906454	6.122518585395332
>5k	4.171034727654675E-4	1.5689274417030834
>10k+	3.128276045741006E-4	1.8894136505700185
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	11702	0.6505444459948599	No Hit
GTCCTACAGTGGACATTTCTAAATT	11448	0.6364239290505175	No Hit
CTGTAGGACGTGGAATATGGCAAGA	10743	0.5972311556420082	No Hit
CTTTAGGACGTGAAATATGGCGAGG	9549	0.53085360748632	No Hit
GTCCTACAGTGTGCATTTCTCATTT	7614	0.42328195281190084	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5799	0.32238140850488745	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5182	0.2880807826991424	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	4708	0.26172989674788927	No Hit
ATTTAGAAATGTCCACTGTAGGACG	4310	0.23960404736265992	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3462	0.1924615340996586	No Hit
GAATATGGCAAGAAAACTGAAAATC	3338	0.18556805338667257	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3248	0.18056472061111817	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2971	0.1651655741796897	No Hit
GATATACACTGTTCTACAAATCCCG	2818	0.15665990846124725	No Hit
TTGTAGAACAGTGTATATCAATGAG	2721	0.15126742758092754	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2507	0.13937061409238707	No Hit
CTGTAGGACATGGAATATGGCAAGA	2455	0.13647979959984458	No Hit
GTGTATATCAATGAGTTACAATGAA	2397	0.13325542958893172	No Hit
GCCATATTCCACGTCCTACAGTGGA	2354	0.13086494837394463	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2324	0.1291971707820932	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2238	0.124416208352119	No Hit
GACCTGGAATATGGCGAGAAAACTG	2238	0.124416208352119	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	2194	0.12197013455073685	No Hit
CCATATTTCACGTCCTAAAGTGTGT	2160	0.1200799866133052	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2128	0.11830102384866364	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2091	0.11624409815204685	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2041	0.1134644688322944	No Hit
CACTTTAGGACGTGAAATATGGCGA	1981	0.1101289136485915	No Hit
GTCCACTGTAGGACGTGGAATATGG	1972	0.10962858037103604	No Hit
ATACACACTTTAGGACGTGAAATAT	1950	0.10840554347034496	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1869	0.10390254397234602	No Hit
GTGTATATCAATGAGTTACAATGAG	1852	0.1029574700036302	No Hit
CCACTGTAGGACGTGGAATATGGCA	1847	0.10267950707165494	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1826	0.10151206275735893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCT	35	0.00216397	16.292923	19
TTCGTGA	35	0.0021663865	16.290201	16
TCGCGTA	55	1.1285349E-5	15.54541	9
TTCGTTC	45	6.73613E-4	14.785555	18
CCGTCGT	65	3.3729357E-6	14.615341	9
CGCGTAA	60	2.568098E-5	14.250354	10
TCGGCGT	90	3.6361598E-8	13.723328	13
ATACGCT	90	3.641435E-8	13.721801	8
TAGGACC	2250	0.0	13.550653	4
GCGTTAT	85	2.7247006E-7	13.401653	1
CGCCACT	65	5.430814E-5	13.159669	17
GTCGAGA	80	1.9899835E-6	13.068282	19
GCGTAAC	80	2.0012121E-6	13.061734	11
CGGCGTC	95	7.376002E-8	13.001772	14
TACGCTA	95	7.3894626E-8	12.999961	9
CGGTCCA	105	1.9934305E-8	12.666982	10
GGGTCGC	75	1.4793541E-5	12.666277	6
GTCGTAG	75	1.4797775E-5	12.665925	11
TAGAAAT	1355	0.0	12.477237	4
AAATGTC	1290	0.0	12.298071	7
>>END_MODULE