FastQCFastQC Report
Thu 2 Feb 2017
SRR4062594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798801
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA62740.34878788704253555No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC45960.2555035270716438No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA45380.252279157060731No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA41980.23337767768641446No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA30240.16811198125862728No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA22420.12463857869769919No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA19000.10562591415059254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC25300.026.4352824
CGTGCGC508.344154E-526.39976510
CGTAGGT508.344154E-526.39976510
CGGGACC450.001398550724.4442314
TCCGTAG752.0653606E-623.466468
ACCGTCG1450.022.758428
ACTGTTC15000.022.7331338
TGTAGGA72350.022.1080762
GTCGTAG1700.021.99980511
GCGAGTA904.0844498E-721.99980520
CTGTAGG71000.021.6941681
AGGACCT48450.021.6598535
GTAGGAC73050.021.383023
CCGTCGT1551.8189894E-1221.2901349
TGACGAG1158.778443E-921.04329118
CGACGGT1900.020.841927
GTATCAA37450.020.7408351
GGACCTG48250.020.6553136
GACGTGT654.933743E-420.3075127
GATATAC17600.020.2537631
Produced by FastQC (version 0.11.5)