##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062594_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1798801
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373587183907503	32.0	32.0	32.0	32.0	32.0
2	31.526933218293742	32.0	32.0	32.0	32.0	32.0
3	31.580974215602502	32.0	32.0	32.0	32.0	32.0
4	31.65647283940803	32.0	32.0	32.0	32.0	32.0
5	31.639365888722544	32.0	32.0	32.0	32.0	32.0
6	35.24363061839525	36.0	36.0	36.0	36.0	36.0
7	35.258937481133266	36.0	36.0	36.0	36.0	36.0
8	35.20373515469471	36.0	36.0	36.0	36.0	36.0
9	35.314582324559524	36.0	36.0	36.0	36.0	36.0
10	35.234785281973934	36.0	36.0	36.0	36.0	36.0
11	35.32394022462741	36.0	36.0	36.0	36.0	36.0
12	35.265044326748765	36.0	36.0	36.0	36.0	36.0
13	35.287405888700306	36.0	36.0	36.0	36.0	36.0
14	35.268126379738504	36.0	36.0	36.0	36.0	36.0
15	35.22905146261315	36.0	36.0	36.0	36.0	36.0
16	35.26173490008067	36.0	36.0	36.0	36.0	36.0
17	35.26196616524007	36.0	36.0	36.0	36.0	36.0
18	35.264632385683576	36.0	36.0	36.0	36.0	36.0
19	35.2280930464237	36.0	36.0	36.0	36.0	36.0
20	35.219370013692455	36.0	36.0	36.0	36.0	36.0
21	35.18774005573713	36.0	36.0	36.0	36.0	36.0
22	35.18047354876943	36.0	36.0	36.0	36.0	36.0
23	35.15825541569078	36.0	36.0	36.0	36.0	36.0
24	35.14138084201643	36.0	36.0	36.0	36.0	36.0
25	35.113443343649465	36.0	36.0	36.0	36.0	36.0
26	35.08152652794834	36.0	36.0	36.0	36.0	36.0
27	35.04043082030753	36.0	36.0	36.0	36.0	36.0
28	35.00121080653168	36.0	36.0	36.0	36.0	36.0
29	34.9756098645709	36.0	36.0	36.0	36.0	36.0
30	34.9354809120075	36.0	36.0	36.0	36.0	36.0
31	34.92585839122838	36.0	36.0	36.0	36.0	36.0
32	34.89400995440852	36.0	36.0	36.0	36.0	36.0
33	34.9206966195816	36.0	36.0	36.0	36.0	36.0
34	34.8950512035517	36.0	36.0	36.0	36.0	36.0
35	34.87702197185792	36.0	36.0	36.0	36.0	36.0
36	34.8240366777648	36.0	36.0	36.0	36.0	36.0
37	34.75883880429242	36.0	36.0	36.0	36.0	36.0
38	34.72914291241777	36.0	36.0	36.0	32.0	36.0
39	34.597518013387806	36.0	36.0	36.0	32.0	36.0
40	34.6511915437005	36.0	36.0	36.0	32.0	36.0
41	34.62108982594517	36.0	36.0	36.0	32.0	36.0
42	34.62408626635187	36.0	36.0	36.0	32.0	36.0
43	34.61352033938162	36.0	36.0	36.0	32.0	36.0
44	34.54627832650749	36.0	36.0	36.0	32.0	36.0
45	34.51783437967846	36.0	36.0	36.0	32.0	36.0
46	34.415284959258976	36.0	36.0	36.0	32.0	36.0
47	34.42276216212911	36.0	36.0	36.0	32.0	36.0
48	34.366734285782584	36.0	36.0	36.0	32.0	36.0
49	34.320075983947085	36.0	36.0	36.0	32.0	36.0
50	33.90633427488644	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	12.0
21	39.0
22	111.0
23	355.0
24	1038.0
25	2320.0
26	4995.0
27	9738.0
28	16935.0
29	26107.0
30	37642.0
31	53757.0
32	78870.0
33	126795.0
34	272165.0
35	1167918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04344734136633	21.531399239032105	12.74668487444252	25.678468545159046
2	11.651233538367114	22.203412633816786	46.19297636996811	19.952377457847987
3	18.08134143656729	27.774924004385177	30.573475028741576	23.57025953030596
4	10.39988925938321	18.308448197073822	40.95473755252118	30.336924991021778
5	10.221697675284815	41.00703746551175	36.76860308616684	12.002661773036595
6	28.707837653179276	39.98263277946131	19.573369001735056	11.736160565624356
7	25.79459961896799	32.69954008202139	24.00212586286739	17.503734436143226
8	22.10016561031487	42.87116807251052	21.076872872541212	13.951793444633399
9	26.362671579568836	15.280734222407036	21.97719480920902	36.3793993888151
10	14.407819430831982	30.41325860948487	35.862610705686734	19.316311253996414
11	32.78811830769496	21.52856263700098	28.88941022381019	16.793908831493866
12	24.60566788655332	24.658314065869433	34.49597815433725	16.240039893239995
13	29.488475934803237	23.83554378722271	26.97936014044911	19.69662013752494
14	18.460018645753475	25.800074605250945	28.440778051602152	27.29912869739343
15	19.61951322019501	38.80857304393315	23.126849495858632	18.44506424001321
16	18.123572312890644	29.24203399931399	33.906196405272176	18.728197282523194
17	18.281510850838973	32.49520096997945	30.784505901431007	18.43878227775057
18	18.477863865986286	28.037731800238046	36.8127991923509	16.671605141424763
19	23.198730710067426	26.79668290155498	28.678936691718537	21.325649696659053
20	23.21802133754651	29.31241421369012	30.46468175190029	17.004882696863078
21	20.819145642013765	27.8518857839194	27.59338025718242	23.735588316884414
22	22.123236533668816	32.66097806260948	28.24381351800449	16.971971885717206
23	18.226918930999037	32.23141414753494	30.66398117412654	18.877685747339477
24	22.52817293297035	28.113949236185658	31.6985592069384	17.65931862390559
25	20.03134310020953	30.447781605636198	31.311912768560834	18.208962525593435
26	16.80847408912937	32.21034455729122	32.469850750583305	18.511330602996107
27	18.497766011915715	31.4661266032207	30.744590424399366	19.291516960464218
28	17.436058796943072	32.26504766230395	28.94355740295897	21.35533613779401
29	19.99832110389087	29.387853353428202	28.299294919226753	22.31453062345418
30	17.896198634534894	29.900305814817756	30.96379199255504	21.23970355809231
31	21.93222040681543	28.00871247013983	29.820030120063308	20.23903700298143
32	19.01205302865631	31.229024222245815	30.32625621177662	19.43266653732125
33	17.605560592861575	31.619006215807083	32.818416267280256	17.957016924051075
34	19.33015380800878	31.058744130117784	32.271551994912166	17.33955006696127
35	18.41888013182114	31.966237510430556	31.462290714759444	18.15259164298886
36	17.22108226535342	30.764659348087974	33.08142479351523	18.932833593043366
37	18.175384603410826	30.339709617684225	29.0015960631554	22.48330971574955
38	19.120069424021892	32.767382272969606	28.901751777989894	19.21079652501861
39	21.70101083999842	28.284562883832066	29.976690028524555	20.037736247644958
40	23.060138392184573	28.180048821409372	31.05012727922655	17.709685507179504
41	21.985255734236304	26.81658504748441	32.67448706110348	18.52367215717581
42	20.012052472730446	31.50893289474489	31.30974465769143	17.169269974833238
43	17.454571128212628	31.702450687986055	32.20133855829522	18.6416396255061
44	16.974640329864172	31.39485690746225	31.033949836585595	20.596552926087988
45	17.38724850608822	32.03984209481761	30.456176086181852	20.116733312912324
46	18.314351202498113	28.19562185171534	31.40982556675378	22.080201379032765
47	21.74466310873916	28.247831887925283	30.42617300422504	19.581331999110517
48	17.763443538223516	29.914759887280475	30.18288293146379	22.13891364303222
49	20.607115517503047	28.522221190670898	30.021275282813388	20.84938800901267
50	17.820213475650434	32.828274405159	28.850789415165668	20.500722704024906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	42.0
2	43.0
3	130.0
4	217.0
5	278.0
6	339.0
7	321.0
8	303.0
9	433.5
10	564.0
11	840.5
12	1117.0
13	1907.5
14	2698.0
15	3687.5
16	4677.0
17	5389.0
18	6101.0
19	7129.0
20	8157.0
21	9901.0
22	11645.0
23	14233.0
24	16821.0
25	21956.5
26	27092.0
27	38570.0
28	50048.0
29	64157.0
30	78266.0
31	97495.5
32	116725.0
33	144938.0
34	173151.0
35	199798.0
36	226445.0
37	223548.5
38	220652.0
39	198829.0
40	177006.0
41	155831.0
42	134656.0
43	119063.0
44	103470.0
45	95217.5
46	86965.0
47	81273.5
48	75582.0
49	69450.5
50	63319.0
51	54834.5
52	46350.0
53	41276.5
54	36203.0
55	33868.5
56	31534.0
57	29047.5
58	26561.0
59	23277.5
60	19994.0
61	17013.0
62	14032.0
63	11977.5
64	9923.0
65	8510.0
66	7097.0
67	6358.0
68	5619.0
69	5504.0
70	5389.0
71	4190.5
72	2992.0
73	2803.5
74	2615.0
75	1843.5
76	1072.0
77	1047.5
78	1023.0
79	905.0
80	787.0
81	651.0
82	515.0
83	479.0
84	443.0
85	368.0
86	293.0
87	237.5
88	182.0
89	117.0
90	52.0
91	34.5
92	17.0
93	11.5
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01678896109130471
2	0.0040582588068385555
3	7.22703623135633E-4
4	1.667777591851461E-4
5	0.0
6	5.559258639504871E-4
7	1.1118517279009742E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.2237034558019483E-4
47	5.559258639504871E-5
48	0.0
49	0.0
50	5.559258639504871E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1798801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.0049186654075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.93141147533517	56.05890906567754
2	10.4460932462545	13.789870701805818
3	2.242366484994725	4.440216523803375
4	0.7460829542553358	1.9698057885308151
5	0.36797490669043154	1.2144076893506421
6	0.22286505658218717	0.8826113959841221
7	0.1644025088371702	0.7595961956930449
8	0.11722474823669636	0.618992797834881
9	0.08888660061242612	0.5280257559480972
>10	0.565634795450447	7.204700823574208
>50	0.05706450831138821	2.635058817004988
>100	0.044579715172710865	5.638637340611539
>500	0.003467702655216642	1.6023096734238487
>1k	0.0018607184979211248	2.3066068945204714
>5k	8.457811354186931E-5	0.3502505362365419
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	6274	0.34878788704253555	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	4596	0.2555035270716438	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	4538	0.252279157060731	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	4198	0.23337767768641446	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3024	0.16811198125862728	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2242	0.12463857869769919	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1900	0.10562591415059254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.111851727900974E-4	0.0
10	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
11	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
12	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
13	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
14	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
15	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
16	1.111851727900974E-4	0.0	0.0	1.111851727900974E-4	0.0
17	1.111851727900974E-4	0.0	0.0	1.667777591851461E-4	0.0
18	1.111851727900974E-4	0.0	0.0	3.335555183702922E-4	0.0
19	1.111851727900974E-4	0.0	0.0	3.335555183702922E-4	0.0
20	1.111851727900974E-4	0.0	0.0	8.338887959257306E-4	0.0
21	1.111851727900974E-4	0.0	0.0	8.338887959257306E-4	0.0
22	1.111851727900974E-4	0.0	0.0	0.0016121850054564124	0.0
23	1.111851727900974E-4	0.0	0.0	0.002279296042196997	0.0
24	1.111851727900974E-4	0.0	0.0	0.004225036566023702	0.0
25	1.111851727900974E-4	0.0	0.0	0.005003332775554383	0.0
26	1.111851727900974E-4	0.0	0.0	0.0063931474354306005	0.0
27	1.111851727900974E-4	0.0	0.0	0.008060925027282062	0.0
28	1.111851727900974E-4	0.0	0.0	0.01328662814841664	0.0
29	1.111851727900974E-4	0.0	0.0	0.021291960589303653	0.0
30	1.111851727900974E-4	0.0	0.0	0.03763618098944797	0.0
31	1.111851727900974E-4	0.0	0.0	0.0920057304838056	0.0
32	1.111851727900974E-4	0.0	0.0	0.16794520349944214	0.0
33	1.111851727900974E-4	0.0	0.0	0.24949952774097858	0.0
34	1.111851727900974E-4	0.0	0.0	0.34834314635137514	0.0
35	1.111851727900974E-4	0.0	0.0	0.45096706083663507	0.0
36	1.111851727900974E-4	0.0	0.0	0.593562044939935	0.0
37	1.111851727900974E-4	0.0	0.0	0.8215472417460297	0.0
38	1.111851727900974E-4	0.0	0.0	1.1056253582247286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	2530	0.0	26.435282	4
CGTGCGC	50	8.344154E-5	26.399765	10
CGTAGGT	50	8.344154E-5	26.399765	10
CGGGACC	45	0.0013985507	24.44423	14
TCCGTAG	75	2.0653606E-6	23.46646	8
ACCGTCG	145	0.0	22.75842	8
ACTGTTC	1500	0.0	22.733133	8
TGTAGGA	7235	0.0	22.108076	2
GTCGTAG	170	0.0	21.999805	11
GCGAGTA	90	4.0844498E-7	21.999805	20
CTGTAGG	7100	0.0	21.694168	1
AGGACCT	4845	0.0	21.659853	5
GTAGGAC	7305	0.0	21.38302	3
CCGTCGT	155	1.8189894E-12	21.290134	9
TGACGAG	115	8.778443E-9	21.043291	18
CGACGGT	190	0.0	20.84192	7
GTATCAA	3745	0.0	20.740835	1
GGACCTG	4825	0.0	20.655313	6
GACGTGT	65	4.933743E-4	20.307512	7
GATATAC	1760	0.0	20.253763	1
>>END_MODULE