FastQCFastQC Report
Thu 2 Feb 2017
SRR4062593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919910
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG54310.28287784323223486No Hit
TCCATGTACTCTGCGTTGATACCAC46170.24048002250105474No Hit
GTATCAACGCAGAGTACTTTTTTTT33170.17276851519081624No Hit
TATCAACGCAGAGTACTTTTTTTTT24510.12766223416722658No Hit
GAGTACATGGAAGCAGTGGTATCAA23960.12479751655025495No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23650.12318285752978005No Hit
GCGCAAGACGGACCAGAGCGAAAGC23430.12203697048299139No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21790.11349490340693054No Hit
CATGTACTCTGCGTTGATACCACTG21750.11328656030751441No Hit
GAATAGGACCGCGGTTCTATTTTGT21680.1129219598835362No Hit
GAATAACGCCGCCGCATCGCCAGTC21480.11188024438645561No Hit
GTCCTATTCCATTATTCCTAGCTGC19550.10182768983962791No Hit
GATTAAGAGGGACGGCCGGGGGCAT19380.10094223166710939No Hit
GTATCTGATCGTCTTCGAACCTCCG19300.10052554546827715No Hit
GGGTAGGCACACGCTGAGCCAGTCA19210.1000567734945909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG707.260549E-613.5721737
CGTCTTA1001.006083E-813.30142315
CGCATCG3200.012.7656613
AGTGATC905.401853E-712.6676938
AACCGCG2050.012.5128817
GTCCTAA1900.012.4925421
GTATTAG3150.012.0562951
GCGTTAT1501.4551915E-1112.02615451
AAGACGG5100.011.9200475
TCTATAC1201.0068106E-811.8710093
GGTTCTA4250.011.84709213
CAAGACG4950.011.7049674
CGGTTCT4250.011.62416812
TCCATTA4100.011.5863048
GGGTAGG5100.011.54212951
CGGTCCA4550.011.48444610
ATCGCCA3700.011.30027516
CGTTATT1604.5474735E-1111.2756952
TGCACCG855.33106E-511.1750445
GCCGTCA600.00587296611.0842318
Produced by FastQC (version 0.11.5)