##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062593_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1919910
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164957732393706	32.0	32.0	32.0	32.0	32.0
2	30.746067784427396	32.0	32.0	32.0	32.0	32.0
3	30.745832877582803	32.0	32.0	32.0	32.0	32.0
4	30.82874561828419	32.0	32.0	32.0	32.0	32.0
5	30.586364465000962	32.0	32.0	32.0	32.0	32.0
6	34.24325931944727	36.0	36.0	36.0	32.0	36.0
7	34.12947169398566	36.0	36.0	36.0	32.0	36.0
8	34.12393445526092	36.0	36.0	36.0	32.0	36.0
9	34.31139063810283	36.0	36.0	36.0	32.0	36.0
10	33.931606689896924	36.0	36.0	36.0	32.0	36.0
11	34.295033621367665	36.0	36.0	36.0	32.0	36.0
12	34.04167278674521	36.0	36.0	36.0	32.0	36.0
13	34.172264845747975	36.0	36.0	36.0	32.0	36.0
14	34.06486606142996	36.0	36.0	36.0	32.0	36.0
15	33.98988910938534	36.0	36.0	36.0	32.0	36.0
16	33.99355073935757	36.0	36.0	36.0	32.0	36.0
17	33.90845508383205	36.0	36.0	36.0	32.0	36.0
18	33.92913886588434	36.0	36.0	36.0	32.0	36.0
19	33.9152543608815	36.0	36.0	36.0	32.0	36.0
20	33.91313446984494	36.0	36.0	36.0	32.0	36.0
21	33.889366168205804	36.0	36.0	36.0	32.0	36.0
22	33.83152543608815	36.0	36.0	36.0	32.0	36.0
23	33.787267632336935	36.0	36.0	36.0	32.0	36.0
24	33.745553697829585	36.0	36.0	36.0	32.0	36.0
25	33.277717705517446	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	71.0
5	190.0
6	311.0
7	76.0
8	284.0
9	365.0
10	234.0
11	76.0
12	143.0
13	121.0
14	354.0
15	376.0
16	651.0
17	854.0
18	1238.0
19	1729.0
20	2689.0
21	3918.0
22	6102.0
23	8928.0
24	12642.0
25	18046.0
26	24875.0
27	31653.0
28	42635.0
29	55869.0
30	72475.0
31	97973.0
32	141649.0
33	202814.0
34	439147.0
35	751409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.0670077880054	16.973851241856348	11.013326235175782	25.945814734962468
2	17.063566267796414	19.678160512417968	36.408886370159166	26.849386849626455
3	19.314915653638725	23.328777355400657	28.06260163702677	29.293705353933852
4	12.734082787218304	14.810952167616506	35.17295089653161	37.282014148633586
5	14.921435006270714	36.2210230200616	33.23958089638654	15.617961077281151
6	35.15161305451191	34.574185916843405	16.497553451483302	13.776647577161377
7	30.731431204270564	30.159614556697463	20.40203854641508	18.70691569261689
8	28.69899495208311	32.40764730997588	19.346090666488724	19.54726707145229
9	27.205498992713334	14.195807768828752	18.357173841411786	40.241519397046126
10	15.962818590704646	26.542937227038653	31.267270712469852	26.226973469786845
11	37.9246883919245	21.008597937058322	21.531712552404027	19.53500111861315
12	24.538493754182173	23.503165545595696	28.46544968271527	23.492891017506857
13	29.729604286363188	19.08070985328468	25.07391045635437	26.11577540399776
14	23.63599004541311	19.421770339419705	24.642504750958015	32.29973486420917
15	25.504610693137042	26.866856197685163	22.21189164610401	25.41664146307378
16	26.291223761364318	25.446477224434034	23.573382140911022	24.688916873290626
17	24.303311603650588	25.704615384615387	25.12208604954368	24.869986962190353
18	25.404731344754804	24.3817745333092	26.17291381012557	24.04058031181042
19	25.61383817974905	25.333974492036454	24.82290772425864	24.229279603955852
20	25.656922068419917	23.919801454049743	25.18120864169267	25.242067835837666
21	26.719467857457644	24.291051178665064	23.968475748951303	25.021005214925985
22	25.69037054502471	24.471130622937547	24.544995498207108	25.293503333830635
23	24.312603734061074	24.161912243201147	25.10387750226637	26.421606520471407
24	24.63969106765562	25.142425884558232	24.945692226569165	25.272190821216984
25	25.119760088662886	24.3975226546711	24.730738640067802	25.751978616598215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172.0
1	172.0
2	494.5
3	817.0
4	817.0
5	817.0
6	2104.5
7	3392.0
8	3392.0
9	3392.0
10	3640.0
11	3888.0
12	3888.0
13	3888.0
14	4470.0
15	5052.0
16	5052.0
17	5052.0
18	8118.5
19	11185.0
20	11185.0
21	11185.0
22	17914.5
23	24644.0
24	24644.0
25	24644.0
26	37137.0
27	49630.0
28	49630.0
29	49630.0
30	62526.0
31	75422.0
32	75422.0
33	75422.0
34	94821.0
35	114220.0
36	114220.0
37	114220.0
38	130843.0
39	147466.0
40	147466.0
41	147466.0
42	171610.0
43	195754.0
44	195754.0
45	195754.0
46	229208.0
47	262662.0
48	262662.0
49	262662.0
50	261067.5
51	259473.0
52	259473.0
53	259473.0
54	240072.0
55	220671.0
56	220671.0
57	220671.0
58	204945.0
59	189219.0
60	189219.0
61	189219.0
62	168314.0
63	147409.0
64	147409.0
65	147409.0
66	122413.5
67	97418.0
68	97418.0
69	97418.0
70	74148.0
71	50878.0
72	50878.0
73	50878.0
74	40070.0
75	29262.0
76	29262.0
77	29262.0
78	23702.0
79	18142.0
80	18142.0
81	18142.0
82	12701.0
83	7260.0
84	7260.0
85	7260.0
86	5397.5
87	3535.0
88	3535.0
89	3535.0
90	2371.0
91	1207.0
92	1207.0
93	1207.0
94	730.0
95	253.0
96	253.0
97	253.0
98	566.0
99	879.0
100	879.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008489981301206827
2	0.003698090014636103
3	0.004583548187154606
4	0.01510487470766859
5	0.03494955492705387
6	0.04583548187154606
7	0.06708647801199015
8	0.08818121682787214
9	0.09823377137469985
10	0.11901599554145767
11	0.1227661713309478
12	0.13266246855321343
13	0.1243287445765687
14	0.12511003119937913
15	0.11427619002974097
16	0.11328656030751441
17	0.12552671739821136
18	0.13162075305613283
19	0.13271455432806747
20	0.12339120062919615
21	0.1317249246058409
22	0.15084040397726975
23	0.14313170929887337
24	0.13526675729591492
25	0.13203743925496508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1919910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.57181349599523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.557543813489	39.7279745443924
2	12.776137329894649	12.922248684933093
3	3.6989251467298123	5.6118405796820054
4	1.535612334717936	3.106348023740211
5	0.8180810874728116	2.0685922090137994
6	0.5220665832042144	1.5841112326977005
7	0.36817109775708073	1.303335606327084
8	0.2520982435282417	1.019925228350258
9	0.1998976143846668	0.9098266385660168
>10	1.080153665320774	9.894557201411308
>50	0.08908368832063712	3.145832738173952
>100	0.08296526345357749	9.11665271019487
>500	0.013567748689144798	4.739590403819224
>1k	0.005592812436746711	4.564701827767413
>5k	1.0357060068049463E-4	0.28446237093063476
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5431	0.28287784323223486	No Hit
TCCATGTACTCTGCGTTGATACCAC	4617	0.24048002250105474	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3317	0.17276851519081624	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2451	0.12766223416722658	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2396	0.12479751655025495	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2365	0.12318285752978005	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2343	0.12203697048299139	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2179	0.11349490340693054	No Hit
CATGTACTCTGCGTTGATACCACTG	2175	0.11328656030751441	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2168	0.1129219598835362	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2148	0.11188024438645561	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1955	0.10182768983962791	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1938	0.10094223166710939	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1930	0.10052554546827715	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1921	0.1000567734945909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.208577485402961E-5
2	0.0	0.0	0.0	0.0	5.208577485402961E-5
3	0.0	0.0	0.0	0.0	5.208577485402961E-5
4	0.0	0.0	0.0	0.0	5.208577485402961E-5
5	0.0	0.0	0.0	5.208577485402961E-5	5.208577485402961E-5
6	0.0	0.0	0.0	5.208577485402961E-5	1.0417154970805923E-4
7	0.0	0.0	0.0	1.5625732456208885E-4	1.0417154970805923E-4
8	0.0	0.0	0.0	1.5625732456208885E-4	1.0417154970805923E-4
9	0.0	0.0	0.0	1.5625732456208885E-4	1.5625732456208885E-4
10	0.0	0.0	0.0	1.5625732456208885E-4	1.5625732456208885E-4
11	0.0	0.0	0.0	2.0834309941611845E-4	1.5625732456208885E-4
12	0.0	0.0	0.0	2.0834309941611845E-4	2.604288742701481E-4
13	0.0	0.0	0.0	2.604288742701481E-4	2.604288742701481E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTG	70	7.260549E-6	13.572173	7
CGTCTTA	100	1.006083E-8	13.301423	15
CGCATCG	320	0.0	12.76566	13
AGTGATC	90	5.401853E-7	12.667693	8
AACCGCG	205	0.0	12.512881	7
GTCCTAA	190	0.0	12.492542	1
GTATTAG	315	0.0	12.056295	1
GCGTTAT	150	1.4551915E-11	12.0261545	1
AAGACGG	510	0.0	11.920047	5
TCTATAC	120	1.0068106E-8	11.871009	3
GGTTCTA	425	0.0	11.847092	13
CAAGACG	495	0.0	11.704967	4
CGGTTCT	425	0.0	11.624168	12
TCCATTA	410	0.0	11.586304	8
GGGTAGG	510	0.0	11.5421295	1
CGGTCCA	455	0.0	11.484446	10
ATCGCCA	370	0.0	11.300275	16
CGTTATT	160	4.5474735E-11	11.275695	2
TGCACCG	85	5.33106E-5	11.175044	5
GCCGTCA	60	0.005872966	11.084231	8
>>END_MODULE