FastQCFastQC Report
Thu 2 Feb 2017
SRR4062593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919910
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24530.12776640571693465No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22320.11625544947419411No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21920.11417201848003293No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21560.11229693058528785No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21390.11141147241276934No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20140.10490075055601565No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20030.10432780703262133No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19410.1010984889916715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3000.020.533214
TCGTTAG951.5979893E-518.52620514
CCGTTAT902.2163267E-417.111019
ATACGAA7400.016.6485540
AGGACGT4000.016.4999035
TATGGCG4400.016.499916
TACGAAT7650.016.3920641
GTATTAC1805.2568794E-915.8921051
GTAGGAC5400.015.8887953
TACGACG6950.015.8272455
AGAGCGA7950.015.77349115
GAGCGAA7850.015.69417516
CGCGGGA5750.015.68686344
ATAACGC7450.015.6509133
AAGACGG8050.015.5775485
CGGTCCA8350.015.54481910
AATCGGT850.002980911915.5293225
ATACCGT6250.015.4879096
TAACGCC7400.015.4593684
AATAACG7550.015.4444222
Produced by FastQC (version 0.11.5)