##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1919910
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290125057945424	32.0	32.0	32.0	32.0	32.0
2	31.427309613471465	32.0	32.0	32.0	32.0	32.0
3	31.508882708043608	32.0	32.0	32.0	32.0	32.0
4	31.607685256079712	32.0	32.0	32.0	32.0	32.0
5	31.54421769770458	32.0	32.0	32.0	32.0	32.0
6	35.14716262741483	36.0	36.0	36.0	36.0	36.0
7	35.157582907532124	36.0	36.0	36.0	36.0	36.0
8	35.10507888390602	36.0	36.0	36.0	36.0	36.0
9	35.227178357318834	36.0	36.0	36.0	36.0	36.0
10	35.107207629524304	36.0	36.0	36.0	36.0	36.0
11	35.239220067607334	36.0	36.0	36.0	36.0	36.0
12	35.14699074435781	36.0	36.0	36.0	36.0	36.0
13	35.186303003786634	36.0	36.0	36.0	36.0	36.0
14	35.148376225968924	36.0	36.0	36.0	36.0	36.0
15	35.112915188732806	36.0	36.0	36.0	36.0	36.0
16	35.12497773333125	36.0	36.0	36.0	36.0	36.0
17	35.09474714960597	36.0	36.0	36.0	36.0	36.0
18	35.10717794063263	36.0	36.0	36.0	36.0	36.0
19	35.086179560500234	36.0	36.0	36.0	36.0	36.0
20	35.08494460677844	36.0	36.0	36.0	36.0	36.0
21	35.0788760931502	36.0	36.0	36.0	36.0	36.0
22	35.05212796433166	36.0	36.0	36.0	36.0	36.0
23	35.013357917819064	36.0	36.0	36.0	36.0	36.0
24	34.986082160101255	36.0	36.0	36.0	36.0	36.0
25	34.95574167539103	36.0	36.0	36.0	32.0	36.0
26	34.90949471589814	36.0	36.0	36.0	32.0	36.0
27	34.89212150569558	36.0	36.0	36.0	32.0	36.0
28	34.87515977311436	36.0	36.0	36.0	32.0	36.0
29	34.84824549067404	36.0	36.0	36.0	32.0	36.0
30	34.83202181352251	36.0	36.0	36.0	32.0	36.0
31	34.834554223895914	36.0	36.0	36.0	32.0	36.0
32	34.79463829033653	36.0	36.0	36.0	32.0	36.0
33	34.77403836638176	36.0	36.0	36.0	32.0	36.0
34	34.7618716502336	36.0	36.0	36.0	32.0	36.0
35	34.72567307842555	36.0	36.0	36.0	32.0	36.0
36	34.70391268340703	36.0	36.0	36.0	32.0	36.0
37	34.68117880525649	36.0	36.0	36.0	32.0	36.0
38	34.6443359324135	36.0	36.0	36.0	32.0	36.0
39	34.60671073123219	36.0	36.0	36.0	32.0	36.0
40	34.59624774077952	36.0	36.0	36.0	32.0	36.0
41	34.564743659859055	36.0	36.0	36.0	32.0	36.0
42	34.507076894229414	36.0	36.0	36.0	32.0	36.0
43	34.521331729091465	36.0	36.0	36.0	32.0	36.0
44	34.48705512237553	36.0	36.0	36.0	32.0	36.0
45	34.42582360631488	36.0	36.0	36.0	32.0	36.0
46	34.4559687693694	36.0	36.0	36.0	32.0	36.0
47	34.41108906146642	36.0	36.0	36.0	32.0	36.0
48	34.38757025068883	36.0	36.0	36.0	32.0	36.0
49	34.38234865175972	36.0	36.0	36.0	32.0	36.0
50	33.87034965180659	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	16.0
21	48.0
22	168.0
23	491.0
24	1369.0
25	3141.0
26	6589.0
27	12228.0
28	20115.0
29	30413.0
30	43375.0
31	61557.0
32	91029.0
33	144013.0
34	323985.0
35	1181368.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46085425845092	17.567148698441276	11.55532217153413	26.41667487157367
2	16.44320035086495	20.130824287813436	36.08023014573709	27.34574521558453
3	18.739901807283513	23.515572181438806	28.39874826683501	29.345777744442675
4	12.502682420199301	15.207447440189842	35.35158976367618	36.93828037593468
5	14.619799886453011	36.479366220291574	33.24030813944404	15.660525753811378
6	35.421734189069674	34.66909317435333	16.21980451126253	13.689368125314468
7	30.699960623113192	30.12706858870321	20.485460761661496	18.6875100265221
8	28.768744368225597	32.35083936226177	19.3233536988713	19.55706257064133
9	27.326124662093537	13.912579235484996	18.31861910193707	40.442677000484395
10	15.879598522847424	26.584214885072736	31.323499539040895	26.212687053038948
11	38.18439406013824	20.838841404024148	21.509185326395507	19.46757920944211
12	24.521722372402873	23.447869952237344	28.5076904646572	23.522717210702584
13	29.868066732294746	18.908021730185272	25.141751436265242	26.082160101254747
14	23.652306618539413	19.22496367017204	24.695324259991352	32.4274054512972
15	25.575209254600477	26.866103098582744	22.15551770655916	25.403169940257612
16	26.353579074019095	25.403378283357032	23.530373819606126	24.712668823017744
17	24.470053283747674	25.69776708283201	25.046903240256054	24.785276393164263
18	25.558802235521455	24.20889520862957	26.145392231927538	24.086910323921433
19	25.69136053252496	25.166596350870613	24.961847169919423	24.180195946685
20	25.77037465297852	23.813824606361756	25.2239427889849	25.191857951674816
21	26.862092493918986	24.11034892260575	24.079462058117308	24.94809652535796
22	25.755582292919982	24.26780421998948	24.73600325015235	25.24061023693819
23	24.355360407519104	23.82538764837935	25.270403300154694	26.548848643946855
24	24.716262741482673	24.943252548296535	25.021329124802726	25.31915558541807
25	25.3099364032689	24.1025881421525	24.876166070284544	25.711309384294058
26	24.70532472876333	24.89653160825247	25.40478459927809	24.993359063706112
27	25.741779562583663	24.544379684464378	24.686261335166755	25.027579417785205
28	24.793922631790032	24.110296836830894	25.437234037012153	25.658546494366924
29	24.59276736930377	24.730117557593843	25.47312113588658	25.203993937215806
30	24.847258465240557	24.767306800839624	25.60495023204213	24.78048450187769
31	25.16399206212791	24.578860467417744	24.53021235370408	25.726935116750266
32	24.92627258569412	24.652822267710466	24.582714814756944	25.838190331838472
33	24.594382028324244	24.04987733800022	25.271601272976334	26.084139360699197
34	25.208369142303543	24.55786990015157	25.436661093488755	24.797099864056126
35	26.038720565026484	24.21587470246001	25.240558151163334	24.50484658135017
36	24.6842820757223	25.02528764369163	24.88903125667349	25.401399023912578
37	25.961946132891644	24.54453594178894	24.75678547431911	24.736732451000307
38	24.842570745503696	24.319577480194386	25.132896854540054	25.704954919761864
39	25.38994015344469	24.21754144725534	24.802204269991822	25.590314129308144
40	25.685474839966453	24.45640681073592	25.07065435358949	24.78746399570813
41	24.50047137626243	24.767150543515058	25.689954216603905	25.042423863618605
42	26.16101796438375	24.912313598033244	24.9686704064253	23.95799803115771
43	25.078675562917013	23.687464516565882	25.201910506221648	26.031949414295465
44	24.774859238193457	24.462709189493257	25.172429957654263	25.59000161465902
45	24.81767374512347	24.762671166877613	25.077998447843907	25.34165664015501
46	24.57839081705042	24.295095697393517	25.519270214270907	25.607243271285157
47	24.908381026590856	24.10813434810418	25.920200342933096	25.06328428237186
48	25.56104192384018	25.259361115885643	24.515628336744953	24.66396862352923
49	24.431041038382006	24.85272747160023	25.333583345052634	25.38264814496513
50	24.380920534171707	25.487849151026587	24.64072478510678	25.49050552969493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	15.5
2	19.0
3	96.0
4	173.0
5	213.0
6	253.0
7	242.0
8	231.0
9	318.0
10	405.0
11	638.0
12	871.0
13	1448.5
14	2026.0
15	2825.5
16	3625.0
17	4121.0
18	4617.0
19	5024.5
20	5432.0
21	6188.5
22	6945.0
23	7576.0
24	8207.0
25	9671.5
26	11136.0
27	13848.5
28	16561.0
29	19268.0
30	21975.0
31	24634.5
32	27294.0
33	31555.0
34	35816.0
35	41858.5
36	47901.0
37	57320.0
38	66739.0
39	73135.5
40	79532.0
41	87672.5
42	95813.0
43	99956.5
44	104100.0
45	116606.0
46	129112.0
47	138388.5
48	147665.0
49	149759.5
50	151854.0
51	145301.5
52	138749.0
53	135913.0
54	133077.0
55	135050.0
56	137023.0
57	133412.0
58	129801.0
59	120027.5
60	110254.0
61	97296.5
62	84339.0
63	73795.5
64	63252.0
65	54263.5
66	45275.0
67	39653.0
68	34031.0
69	31273.5
70	28516.0
71	22088.0
72	15660.0
73	14196.0
74	12732.0
75	9343.0
76	5954.0
77	5322.0
78	4690.0
79	4004.5
80	3319.0
81	2634.5
82	1950.0
83	1711.5
84	1473.0
85	1179.5
86	886.0
87	656.5
88	427.0
89	274.0
90	121.0
91	80.0
92	39.0
93	24.5
94	10.0
95	8.5
96	7.0
97	8.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017032048377267683
2	0.004323119312884458
3	6.250292982483554E-4
4	1.0417154970805924E-4
5	0.0
6	2.0834309941611848E-4
7	1.0417154970805924E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.0834309941611848E-4
47	1.0417154970805924E-4
48	0.0
49	0.0
50	1.5625732456208885E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1919910.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.623389625270626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89276785157804	42.304976291211936
2	12.832912297513735	13.762885123130117
3	3.63712568357712	5.85105022939604
4	1.4496037802652764	3.1093067324572035
5	0.764548795325549	2.04988489696366
6	0.49042330761530145	1.5778896063341554
7	0.3405055024359669	1.278134145867067
8	0.24579163973977566	1.0544144691520114
9	0.18127690399794347	0.8748613847830051
>10	1.0008888337944963	9.67843929333042
>50	0.07619946909694042	2.8604260991926935
>100	0.07308579128096777	8.421139185999769
>500	0.010859118154350169	3.9912392640040526
>1k	0.004011025624579792	3.1853532781778355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2453	0.12776640571693465	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2232	0.11625544947419411	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2192	0.11417201848003293	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2156	0.11229693058528785	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2139	0.11141147241276934	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2014	0.10490075055601565	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2003	0.10432780703262133	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1941	0.1010984889916715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.208577485402961E-5
2	0.0	0.0	0.0	0.0	5.208577485402961E-5
3	0.0	0.0	0.0	0.0	5.208577485402961E-5
4	0.0	0.0	0.0	0.0	5.208577485402961E-5
5	0.0	0.0	0.0	5.208577485402961E-5	5.208577485402961E-5
6	0.0	0.0	0.0	5.208577485402961E-5	5.208577485402961E-5
7	0.0	0.0	0.0	1.0417154970805923E-4	5.208577485402961E-5
8	0.0	0.0	0.0	1.0417154970805923E-4	5.208577485402961E-5
9	0.0	0.0	0.0	1.0417154970805923E-4	5.208577485402961E-5
10	0.0	0.0	0.0	1.0417154970805923E-4	5.208577485402961E-5
11	0.0	0.0	0.0	1.0417154970805923E-4	5.208577485402961E-5
12	0.0	0.0	0.0	1.0417154970805923E-4	1.5625732456208885E-4
13	0.0	0.0	0.0	1.0417154970805923E-4	1.5625732456208885E-4
14	0.0	0.0	0.0	1.0417154970805923E-4	1.5625732456208885E-4
15	0.0	0.0	0.0	1.0417154970805923E-4	2.0834309941611845E-4
16	0.0	0.0	0.0	1.5625732456208885E-4	2.604288742701481E-4
17	0.0	0.0	0.0	2.0834309941611845E-4	2.604288742701481E-4
18	0.0	0.0	0.0	2.604288742701481E-4	3.125146491241777E-4
19	0.0	0.0	0.0	2.604288742701481E-4	3.6460042397820733E-4
20	0.0	0.0	0.0	3.6460042397820733E-4	3.6460042397820733E-4
21	0.0	0.0	0.0	6.250292982483554E-4	3.6460042397820733E-4
22	0.0	0.0	0.0	8.333723976644738E-4	3.6460042397820733E-4
23	0.0	0.0	0.0	0.0014584016959128293	4.166861988322369E-4
24	0.0	0.0	0.0	0.0025001171929934216	4.166861988322369E-4
25	0.0	0.0	0.0	0.003229318040949836	4.166861988322369E-4
26	0.0	0.0	0.0	0.004166861988322369	6.250292982483554E-4
27	0.0	0.0	0.0	0.005833606783651317	6.250292982483554E-4
28	0.0	0.0	0.0	0.011302613143324427	6.250292982483554E-4
29	0.0	0.0	0.0	0.019480079795407078	6.250292982483554E-4
30	0.0	0.0	0.0	0.037449672120047295	6.250292982483554E-4
31	0.0	0.0	0.0	0.07672234635998562	6.77115073102385E-4
32	0.0	0.0	0.0	0.1227661713309478	7.292008479564147E-4
33	0.0	0.0	0.0	0.17157054236917355	7.292008479564147E-4
34	0.0	0.0	0.0	0.22813569386064972	7.812866228104442E-4
35	0.0	0.0	0.0	0.29350334130245687	7.812866228104442E-4
36	0.0	0.0	0.0	0.3914766838028866	7.812866228104442E-4
37	0.0	0.0	0.0	0.5367959956456292	7.812866228104442E-4
38	0.0	0.0	0.0	0.7187316072107547	7.812866228104442E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	300	0.0	20.53321	4
TCGTTAG	95	1.5979893E-5	18.526205	14
CCGTTAT	90	2.2163267E-4	17.11101	9
ATACGAA	740	0.0	16.64855	40
AGGACGT	400	0.0	16.499903	5
TATGGCG	440	0.0	16.4999	16
TACGAAT	765	0.0	16.39206	41
GTATTAC	180	5.2568794E-9	15.892105	1
GTAGGAC	540	0.0	15.888795	3
TACGACG	695	0.0	15.827245	5
AGAGCGA	795	0.0	15.773491	15
GAGCGAA	785	0.0	15.694175	16
CGCGGGA	575	0.0	15.686863	44
ATAACGC	745	0.0	15.650913	3
AAGACGG	805	0.0	15.577548	5
CGGTCCA	835	0.0	15.544819	10
AATCGGT	85	0.0029809119	15.52932	25
ATACCGT	625	0.0	15.487909	6
TAACGCC	740	0.0	15.459368	4
AATAACG	755	0.0	15.444422	2
>>END_MODULE