FastQCFastQC Report
Thu 2 Feb 2017
SRR4062592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690635
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG46390.6717006812571039No Hit
TCCATGTACTCTGCGTTGATACCAC37700.5458744488767584No Hit
GAGTACATGGAAGCAGTGGTATCAA21720.3144931838091032No Hit
GAATAATGTAGATAAGGGAAGTCGG19470.28191447001672376No Hit
ACATTATTCTATGGACTAGAGGCTG18870.27322681300542256No Hit
GCTTATAACCCTGGAATCGGATTAT18360.26584230454581653No Hit
TCCGAAGACTGGCCAAGGGCGCCTG17750.2570098532509937No Hit
ACCCTACCCTATGAGCCAATCCTTT17280.2502045219254744No Hit
CATGTACTCTGCGTTGATACCACTG16700.24180645348121657No Hit
GGGTAGGGTACGTCGGGGCCTTGGG16030.23210523648526357No Hit
GTCCTAAGAGATAGGGGAACTCCGT15170.21965292810239853No Hit
GTTCATAGTTGGTAGTTAAGCGTAC14360.20792459113714193No Hit
GTATCAACGCAGAGTACTTTTTTTT14110.20430473404909974No Hit
GTCCACAACGGCCCTTGAAAATCCG13830.20025049411049253No Hit
GTTATAAGCTGTAAAGGAGAAAAGA13590.19677543130597205No Hit
GTCCGTACCAGTTCATAGTTGGTAG13500.19547228275427686No Hit
GCTTCCATGTACTCTGCGTTGATAC13460.1948931056201901No Hit
ATCGTACCCATATCCGCATCAGGTC12650.1831647686549335No Hit
CCATAGAATAATGTAGATAAGGGAA11730.16984369457093834No Hit
ATTCTATGGACTAGAGGCTGATCAC11670.16897492886980822No Hit
GGCCAAGGGCGCCTGCTCGGGTCCA11150.16144562612668054No Hit
GTATCAACGCAGAGTACATGGGCAG10680.15464029480116126No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10630.15391632338355282No Hit
GTTTTAATTAGACAGTCAGATTCCC10520.15232358626481426No Hit
CCCCTTGTCCGTACCAGTTCATAGT10460.15145482056368414No Hit
CATGGAAGCAGTGGTATCAACGCAG10370.15015167201198898No Hit
CCCATGTACTCTGCGTTGATACCAC10290.14899331774381547No Hit
GGGTACGATCGCATGTAAGACTTTT10280.14884852346029379No Hit
GCGTTGATACCACTGCTTCCATGTA10250.14841414060972874No Hit
GTCTAATGGCTGGTAGAGCATTGCT9900.1433463406864697No Hit
TATCAACGCAGAGTACTTTTTTTTT9850.14262236926886127No Hit
GTACATGGGCAGAAATCACATTGCG9820.1421879864182962No Hit
TCGCAATGCTATGTTTTAATTAGAC9520.1378441579126456No Hit
GACTAGAGGCTGATCACCTTGGGGA9440.13668580364447214No Hit
GTTGTGGACGCACCGGACATTACAA9330.13509306652573358No Hit
CCTTGGGGACCTGATGCGGATATGG9330.13509306652573358No Hit
GTCCCATCTCGGTCCCTCACATTTG8920.12915650090134442No Hit
ATTTTGTAATGTCCGGTGCGTCCAC8900.12886691233430103No Hit
GTACATGGGAAGCAGTGGTATCAAC8880.12857732376725767No Hit
GGTCCAAAACAGGTCCGCCGCAGCT8420.12191678672526009No Hit
GTATCAACGCAGAGTACATGGAAGC8340.12075843245708659No Hit
GTTTTGGACCCGAGCAGGCGCCCTT8310.12032404960652153No Hit
GCCTATCGAAAGGGAATACGGTTAA8290.12003446103947817No Hit
CTATGAGCCAATCCTTTTCCCGAAG8260.11960007818891309No Hit
GTCAGATTCCCCTTGTCCGTACCAG8030.11626980966791432No Hit
GGGTACGTCGGGGCCTTGGGCAAAT7980.11554583825030589No Hit
GTCTTCGGACGTCTGGCGTACGCTT7940.11496666111621913No Hit
AGATGGGACTACTGCGGCGCAAGCT7880.11409789541508902No Hit
GACGTACCCTACCCTATGAGCCAAT7620.11033324404352517No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7590.10989886119296009No Hit
GTTTCAAAGTGTGCGCTTGTTCGCA7560.10946447834239503No Hit
ACGCAGAGTACATGGAAGCAGTGGT7420.10743735837309143No Hit
CCTTTACAGCTTATAACCCTGGAAT7410.10729256408956973No Hit
GGCTCATAGGGTAGGGTACGTCGGG7400.10714776980604807No Hit
CAGTGGTATCAACGCAGAGTACATG7030.10179038131574566No Hit
GTGTGGGCCCTGGGAAGAGTTATCT6980.10106640989813723No Hit
CCATTAGACCCTATCTCCGGATAAT6930.10034243848052879No Hit
GTCTTACATGCGATCGTACCCATAT6910.10005284991348543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC350.002176805416.2764593
AAGAATT653.3575634E-614.61873216
CCGAATG801.280132E-714.25429718
ATACTGT400.00526969114.252236
GTTAGGG400.005295114.2419013
TAACCTA954.8912625E-913.998135
TCTGGAC759.712348E-713.9254153
AACTAAC707.236833E-613.57355210
CGTCTTA500.001497943313.30015215
AGCGAAT500.001497943313.30015215
ATACCGT655.4359225E-513.1559066
GACGTGA801.991304E-613.0645447
CTTTAGG802.0092193E-613.054131
AACCTAT957.3483534E-813.0020346
AAATCCG2650.012.90955219
TAAGCTG2300.012.8026385
TTAATGA604.0965143E-412.6631394
CTGACGT604.0965143E-412.6631394
AAAATCC2950.012.56310918
CGTCTGG1600.012.470710
Produced by FastQC (version 0.11.5)