##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062592_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690635
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139570105772226	32.0	32.0	32.0	32.0	32.0
2	30.820281335292883	32.0	32.0	32.0	32.0	32.0
3	30.825366510530163	32.0	32.0	32.0	32.0	32.0
4	30.882480615665294	32.0	32.0	32.0	32.0	32.0
5	30.683948829700203	32.0	32.0	32.0	32.0	32.0
6	34.30434455247707	36.0	36.0	36.0	32.0	36.0
7	34.20084415067293	36.0	36.0	36.0	32.0	36.0
8	34.16012510226096	36.0	36.0	36.0	32.0	36.0
9	34.35170531467418	36.0	36.0	36.0	32.0	36.0
10	34.018364258979055	36.0	36.0	36.0	32.0	36.0
11	34.3506989944037	36.0	36.0	36.0	32.0	36.0
12	34.154944362796556	36.0	36.0	36.0	32.0	36.0
13	34.2513237817371	36.0	36.0	36.0	32.0	36.0
14	34.16356975826594	36.0	36.0	36.0	32.0	36.0
15	34.11455689329385	36.0	36.0	36.0	32.0	36.0
16	34.08662607600252	36.0	36.0	36.0	32.0	36.0
17	34.00037356925149	36.0	36.0	36.0	32.0	36.0
18	34.03310286909873	36.0	36.0	36.0	32.0	36.0
19	33.99659443845157	36.0	36.0	36.0	32.0	36.0
20	34.00448717484634	36.0	36.0	36.0	32.0	36.0
21	33.96841457499258	36.0	36.0	36.0	32.0	36.0
22	33.92594641163567	36.0	36.0	36.0	32.0	36.0
23	33.87671345935262	36.0	36.0	36.0	32.0	36.0
24	33.83250776459345	36.0	36.0	36.0	32.0	36.0
25	33.338307499619916	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	31.0
5	67.0
6	105.0
7	27.0
8	95.0
9	129.0
10	84.0
11	33.0
12	52.0
13	49.0
14	103.0
15	159.0
16	242.0
17	371.0
18	486.0
19	654.0
20	977.0
21	1355.0
22	2048.0
23	2909.0
24	4024.0
25	5541.0
26	7925.0
27	10401.0
28	14217.0
29	19095.0
30	25320.0
31	35108.0
32	50676.0
33	72566.0
34	158675.0
35	277105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.02321823168418	17.493317192265028	10.967189803324171	24.516274772726614
2	17.50242176153649	19.13604290680888	36.75502777966821	26.606507551986425
3	19.71856071521142	23.585237614629744	28.15971201561557	28.536489654543267
4	13.038384394256461	14.284555730143445	36.01268618350072	36.664373692099375
5	14.027581232247533	36.88529932777808	33.77317688228657	15.313942557687824
6	33.009130693645886	36.09035953464318	16.749600541206906	14.150909230504022
7	30.02009588650098	30.751052969395428	20.506787251644834	18.722063892458753
8	29.090637694139264	32.39019003438966	18.881270895739767	19.637901375731307
9	26.87599463152616	14.258341860471857	19.13011592077153	39.73554758723045
10	16.16087375037692	26.902123489434743	30.763197875350823	26.17380488483752
11	37.05543724549041	22.333928185709915	20.534149407008048	20.07648516179163
12	24.059411252122295	23.1461169405293	30.36460727184679	22.429864535501615
13	29.50823712242898	20.643980029109716	24.999130187705493	24.84865266075581
14	23.562951773168912	20.03720015889078	25.141856412605723	31.257991655334582
15	25.8510047676271	27.410159437915755	22.46075658719655	24.278079207260596
16	26.39835330100817	26.262384669464318	23.621433178955304	23.717828850572214
17	23.76003943286265	25.563078082867	26.359128997651425	24.317753486618923
18	24.86625278730641	25.611322625823867	25.918397527162647	23.604027059707075
19	25.751418310669845	26.327731616330475	23.84472742192948	24.076122651070204
20	25.3120147986854	24.29677974922839	25.974824258362318	24.416381193723897
21	25.904608363078584	24.670530891081643	23.981291663887866	25.443569081951907
22	23.826352011740106	25.21091876183654	26.234262661651215	24.728466564772145
23	23.753257053868808	24.899044017533353	25.367682775640642	25.980016152957198
24	23.956857792811277	27.01201503800823	24.626695799303775	24.40443136987672
25	25.991173460067447	24.774769550177023	24.964841722509128	24.269215267246402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	92.0
2	237.0
3	382.0
4	382.0
5	382.0
6	1011.0
7	1640.0
8	1640.0
9	1640.0
10	1602.5
11	1565.0
12	1565.0
13	1565.0
14	1453.5
15	1342.0
16	1342.0
17	1342.0
18	2218.0
19	3094.0
20	3094.0
21	3094.0
22	5075.0
23	7056.0
24	7056.0
25	7056.0
26	11864.5
27	16673.0
28	16673.0
29	16673.0
30	23914.5
31	31156.0
32	31156.0
33	31156.0
34	36659.5
35	42163.0
36	42163.0
37	42163.0
38	51193.5
39	60224.0
40	60224.0
41	60224.0
42	65698.0
43	71172.0
44	71172.0
45	71172.0
46	88843.5
47	106515.0
48	106515.0
49	106515.0
50	100358.5
51	94202.0
52	94202.0
53	94202.0
54	87159.0
55	80116.0
56	80116.0
57	80116.0
58	71486.0
59	62856.0
60	62856.0
61	62856.0
62	56391.0
63	49926.0
64	49926.0
65	49926.0
66	39746.5
67	29567.0
68	29567.0
69	29567.0
70	23136.5
71	16706.0
72	16706.0
73	16706.0
74	12546.0
75	8386.0
76	8386.0
77	8386.0
78	5854.0
79	3322.0
80	3322.0
81	3322.0
82	2333.0
83	1344.0
84	1344.0
85	1344.0
86	952.5
87	561.0
88	561.0
89	561.0
90	386.5
91	212.0
92	212.0
93	212.0
94	132.0
95	52.0
96	52.0
97	52.0
98	181.5
99	311.0
100	311.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008253274160736134
2	0.003185474237477104
3	0.006081359907910836
4	0.017375314022602388
5	0.0344610394781614
6	0.047202936428069824
7	0.06428866188362883
8	0.08716615868005531
9	0.09860490707826854
10	0.11974487247243479
11	0.11988966675595648
12	0.13523786080925523
13	0.12032404960652153
14	0.12350952384399863
15	0.11207077544578539
16	0.11149159831169864
17	0.12379911241104202
18	0.1304596494530396
19	0.13118362087064803
20	0.12220637529230347
21	0.12872211805077935
22	0.14957249487790222
23	0.14160880928420944
24	0.1326315637058649
25	0.12988047231895283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	690635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.22563650438222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02224044069348	35.83260154500768
2	11.604535141162394	10.264359059107557
3	3.112931546020004	4.1301413705191585
4	1.2339394194090225	2.1828702492484746
5	0.667119384759792	1.4751889707706833
6	0.41258931447794145	1.0948215028616208
7	0.2769571720610826	0.857402505319856
8	0.19365870989932255	0.6851743767932036
9	0.1340329481397042	0.5334923199633544
>10	0.967480445275884	8.657137435028465
>50	0.166889354623588	5.1440314173832515
>100	0.17970172395939593	16.710801058856717
>500	0.018397251447397015	5.763552611843456
>1k	0.009527148070973454	6.668425577296606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4639	0.6717006812571039	No Hit
TCCATGTACTCTGCGTTGATACCAC	3770	0.5458744488767584	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2172	0.3144931838091032	No Hit
GAATAATGTAGATAAGGGAAGTCGG	1947	0.28191447001672376	No Hit
ACATTATTCTATGGACTAGAGGCTG	1887	0.27322681300542256	No Hit
GCTTATAACCCTGGAATCGGATTAT	1836	0.26584230454581653	No Hit
TCCGAAGACTGGCCAAGGGCGCCTG	1775	0.2570098532509937	No Hit
ACCCTACCCTATGAGCCAATCCTTT	1728	0.2502045219254744	No Hit
CATGTACTCTGCGTTGATACCACTG	1670	0.24180645348121657	No Hit
GGGTAGGGTACGTCGGGGCCTTGGG	1603	0.23210523648526357	No Hit
GTCCTAAGAGATAGGGGAACTCCGT	1517	0.21965292810239853	No Hit
GTTCATAGTTGGTAGTTAAGCGTAC	1436	0.20792459113714193	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1411	0.20430473404909974	No Hit
GTCCACAACGGCCCTTGAAAATCCG	1383	0.20025049411049253	No Hit
GTTATAAGCTGTAAAGGAGAAAAGA	1359	0.19677543130597205	No Hit
GTCCGTACCAGTTCATAGTTGGTAG	1350	0.19547228275427686	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1346	0.1948931056201901	No Hit
ATCGTACCCATATCCGCATCAGGTC	1265	0.1831647686549335	No Hit
CCATAGAATAATGTAGATAAGGGAA	1173	0.16984369457093834	No Hit
ATTCTATGGACTAGAGGCTGATCAC	1167	0.16897492886980822	No Hit
GGCCAAGGGCGCCTGCTCGGGTCCA	1115	0.16144562612668054	No Hit
GTATCAACGCAGAGTACATGGGCAG	1068	0.15464029480116126	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1063	0.15391632338355282	No Hit
GTTTTAATTAGACAGTCAGATTCCC	1052	0.15232358626481426	No Hit
CCCCTTGTCCGTACCAGTTCATAGT	1046	0.15145482056368414	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1037	0.15015167201198898	No Hit
CCCATGTACTCTGCGTTGATACCAC	1029	0.14899331774381547	No Hit
GGGTACGATCGCATGTAAGACTTTT	1028	0.14884852346029379	No Hit
GCGTTGATACCACTGCTTCCATGTA	1025	0.14841414060972874	No Hit
GTCTAATGGCTGGTAGAGCATTGCT	990	0.1433463406864697	No Hit
TATCAACGCAGAGTACTTTTTTTTT	985	0.14262236926886127	No Hit
GTACATGGGCAGAAATCACATTGCG	982	0.1421879864182962	No Hit
TCGCAATGCTATGTTTTAATTAGAC	952	0.1378441579126456	No Hit
GACTAGAGGCTGATCACCTTGGGGA	944	0.13668580364447214	No Hit
GTTGTGGACGCACCGGACATTACAA	933	0.13509306652573358	No Hit
CCTTGGGGACCTGATGCGGATATGG	933	0.13509306652573358	No Hit
GTCCCATCTCGGTCCCTCACATTTG	892	0.12915650090134442	No Hit
ATTTTGTAATGTCCGGTGCGTCCAC	890	0.12886691233430103	No Hit
GTACATGGGAAGCAGTGGTATCAAC	888	0.12857732376725767	No Hit
GGTCCAAAACAGGTCCGCCGCAGCT	842	0.12191678672526009	No Hit
GTATCAACGCAGAGTACATGGAAGC	834	0.12075843245708659	No Hit
GTTTTGGACCCGAGCAGGCGCCCTT	831	0.12032404960652153	No Hit
GCCTATCGAAAGGGAATACGGTTAA	829	0.12003446103947817	No Hit
CTATGAGCCAATCCTTTTCCCGAAG	826	0.11960007818891309	No Hit
GTCAGATTCCCCTTGTCCGTACCAG	803	0.11626980966791432	No Hit
GGGTACGTCGGGGCCTTGGGCAAAT	798	0.11554583825030589	No Hit
GTCTTCGGACGTCTGGCGTACGCTT	794	0.11496666111621913	No Hit
AGATGGGACTACTGCGGCGCAAGCT	788	0.11409789541508902	No Hit
GACGTACCCTACCCTATGAGCCAAT	762	0.11033324404352517	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	759	0.10989886119296009	No Hit
GTTTCAAAGTGTGCGCTTGTTCGCA	756	0.10946447834239503	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	742	0.10743735837309143	No Hit
CCTTTACAGCTTATAACCCTGGAAT	741	0.10729256408956973	No Hit
GGCTCATAGGGTAGGGTACGTCGGG	740	0.10714776980604807	No Hit
CAGTGGTATCAACGCAGAGTACATG	703	0.10179038131574566	No Hit
GTGTGGGCCCTGGGAAGAGTTATCT	698	0.10106640989813723	No Hit
CCATTAGACCCTATCTCCGGATAAT	693	0.10034243848052879	No Hit
GTCTTACATGCGATCGTACCCATAT	691	0.10005284991348543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4479428352168655E-4
2	0.0	0.0	0.0	0.0	1.4479428352168655E-4
3	0.0	0.0	0.0	0.0	1.4479428352168655E-4
4	0.0	0.0	0.0	0.0	1.4479428352168655E-4
5	0.0	0.0	0.0	0.0	1.4479428352168655E-4
6	0.0	0.0	0.0	0.0	1.4479428352168655E-4
7	0.0	0.0	0.0	0.0	1.4479428352168655E-4
8	0.0	0.0	0.0	0.0	1.4479428352168655E-4
9	0.0	0.0	0.0	0.0	1.4479428352168655E-4
10	0.0	0.0	0.0	1.4479428352168655E-4	1.4479428352168655E-4
11	0.0	0.0	0.0	1.4479428352168655E-4	1.4479428352168655E-4
12	0.0	0.0	0.0	1.4479428352168655E-4	4.343828505650597E-4
13	0.0	0.0	0.0	1.4479428352168655E-4	4.343828505650597E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGAC	35	0.0021768054	16.276459	3
AAGAATT	65	3.3575634E-6	14.618732	16
CCGAATG	80	1.280132E-7	14.254297	18
ATACTGT	40	0.005269691	14.25223	6
GTTAGGG	40	0.0052951	14.241901	3
TAACCTA	95	4.8912625E-9	13.99813	5
TCTGGAC	75	9.712348E-7	13.925415	3
AACTAAC	70	7.236833E-6	13.573552	10
CGTCTTA	50	0.0014979433	13.300152	15
AGCGAAT	50	0.0014979433	13.300152	15
ATACCGT	65	5.4359225E-5	13.155906	6
GACGTGA	80	1.991304E-6	13.064544	7
CTTTAGG	80	2.0092193E-6	13.05413	1
AACCTAT	95	7.3483534E-8	13.002034	6
AAATCCG	265	0.0	12.909552	19
TAAGCTG	230	0.0	12.802638	5
TTAATGA	60	4.0965143E-4	12.663139	4
CTGACGT	60	4.0965143E-4	12.663139	4
AAAATCC	295	0.0	12.563109	18
CGTCTGG	160	0.0	12.4707	10
>>END_MODULE