FastQCFastQC Report
Thu 2 Feb 2017
SRR4062592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690635
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATAACCCTGGAATCGGATTATCCGGAGATAGGGTCTAATGGCTGGT18590.26917257306681536No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA18470.26743504166455506No Hit
ACATTATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGAT18110.26222244745777434No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17040.2467294591209539No Hit
TCCGAAGACTGGCCAAGGGCGCCTGCTCGGGTCCAAAACAGGTCCGCCGC16630.24079289349656477No Hit
ACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGC15860.22964373366539487No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15740.22790620226313466No Hit
GGGTAGGGTACGTCGGGGCCTTGGGCAAATGTGAGGGACCGAGATGGGAC13070.18924612856284434No Hit
ATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGG11870.17187081454024195No Hit
GTTCATAGTTGGTAGTTAAGCGTACGCCAGACGTCCGAAGACTGGCCAAG11790.17071246027206846No Hit
GTCCACAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACATGCG11690.16926451743685159No Hit
ATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCA11440.16564466034880942No Hit
GTCCGTACCAGTTCATAGTTGGTAGTTAAGCGTACGCCAGACGTCCGAAG11260.1630383632454191No Hit
GTTATAAGCTGTAAAGGAGAAAAGATAACTCTTCCCAGGGCCCACACCGG11230.162603980394854No Hit
CCATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC10850.15710179762102994No Hit
GTCCTAAGAGATAGGGGAACTCCGTTTCAAAGTGTGCGCTTGTTCGCACG10820.15666741477046486No Hit
GTCTAATGGCTGGTAGAGCATTGCTATTTTGTAATGTCCGGTGCGTCCAC9830.1423327807018179No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA9470.13712018649503718No Hit
GGCCAAGGGCGCCTGCTCGGGTCCAAAACAGGTCCGCCGCAGCTTGCGCC9450.1368305979279938No Hit
CCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAAGCGTACGCCAGACGT9330.13509306652573358No Hit
GACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCG9030.13074923802008298No Hit
ATTTTGTAATGTCCGGTGCGTCCACAACGGCCCTTGAAAATCCGAGGGAA8650.12524705524625887No Hit
GGGTACGATCGCATGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCC8600.12452308382865043No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCATAGTTGG8560.12394390669456369No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT8430.12206158100878178No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA8270.11974487247243479No Hit
CCTTGGGGACCTGATGCGGATATGGGTACGATCGCATGTAAGACTTTTTC8210.11887610677130467No Hit
GTTGTGGACGCACCGGACATTACAAAATAGCAATGCTCTACCAGCCATTA7910.11453227826565407No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA7710.11163639259522033No Hit
GCCTATCGAAAGGGAATACGGTTAAAATTCCGTAACCGCAATGTAGATTC7580.10975406690943842No Hit
GACGTACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTAT7580.10975406690943842No Hit
GTTTCAAAGTGTGCGCTTGTTCGCACGGCCTATCGAAAGGGAATACGGTT7400.10714776980604807No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAAGCGTACG7330.10613420982139624No Hit
GTCCCATCTCGGTCCCTCACATTTGCCCAAGGCCCCGACGTACCCTACCC7290.10555503268730951No Hit
CTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC7210.10439667841913602No Hit
AGATGGGACTACTGCGGCGCAAGCTGCGGCGGACCTGTTTTGGACCCGAG7100.10280394130039747No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7070.10236955844983239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCG300.00574342329.3332375
TAGGACG2150.026.6045634
GTAGGAC2750.024.7999173
CTATAGA450.001396455824.4479031
CGTGGAA1600.021.9999289
CGGTTAT602.8711156E-421.99992843
ACGTGGA1600.021.9999288
GAATATG3650.021.09582113
AGCGATT1158.754796E-921.0434126
GTTCTAA755.4623273E-520.536241
TAGAGTG1502.1827873E-1120.5332645
GGACGTG2900.020.482696
GCGATTT1201.4288162E-820.166627
ACGCACC2950.020.1355278
GACGTGG1750.020.114227
CTGGTCG550.00448178319.9999359
CGAACGT1001.1220436E-619.7999344
GGCGTTA1001.1220436E-619.79993442
TAACACT1001.1220436E-619.7999344
CTGTAGG3150.019.5583231
Produced by FastQC (version 0.11.5)