##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690635
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188047231895286	32.0	32.0	32.0	32.0	32.0
2	31.32551058084227	32.0	32.0	32.0	32.0	32.0
3	31.4605500734831	32.0	32.0	32.0	32.0	32.0
4	31.573347716232163	32.0	32.0	32.0	32.0	32.0
5	31.477354898028626	32.0	32.0	32.0	32.0	32.0
6	35.069980525168866	36.0	36.0	36.0	36.0	36.0
7	35.10484554069805	36.0	36.0	36.0	36.0	36.0
8	35.01776336270244	36.0	36.0	36.0	36.0	36.0
9	35.180141464015	36.0	36.0	36.0	36.0	36.0
10	35.042974943349236	36.0	36.0	36.0	36.0	36.0
11	35.18228441941112	36.0	36.0	36.0	36.0	36.0
12	35.10491504195414	36.0	36.0	36.0	36.0	36.0
13	35.13413308042598	36.0	36.0	36.0	36.0	36.0
14	35.10879697669536	36.0	36.0	36.0	36.0	36.0
15	35.05728496238969	36.0	36.0	36.0	36.0	36.0
16	35.0588067503095	36.0	36.0	36.0	36.0	36.0
17	35.03185763826044	36.0	36.0	36.0	36.0	36.0
18	35.05308158433905	36.0	36.0	36.0	36.0	36.0
19	35.025573566355604	36.0	36.0	36.0	36.0	36.0
20	35.019537092675584	36.0	36.0	36.0	36.0	36.0
21	34.99616150354384	36.0	36.0	36.0	36.0	36.0
22	34.966025469314474	36.0	36.0	36.0	36.0	36.0
23	34.9265646832263	36.0	36.0	36.0	32.0	36.0
24	34.89954896580683	36.0	36.0	36.0	32.0	36.0
25	34.86501987301541	36.0	36.0	36.0	32.0	36.0
26	34.80811571959139	36.0	36.0	36.0	32.0	36.0
27	34.783297979395776	36.0	36.0	36.0	32.0	36.0
28	34.74674031869222	36.0	36.0	36.0	32.0	36.0
29	34.72575673112426	36.0	36.0	36.0	32.0	36.0
30	34.71387925604697	36.0	36.0	36.0	32.0	36.0
31	34.687859723298125	36.0	36.0	36.0	32.0	36.0
32	34.650617185633514	36.0	36.0	36.0	32.0	36.0
33	34.6148341743468	36.0	36.0	36.0	32.0	36.0
34	34.59253151085595	36.0	36.0	36.0	32.0	36.0
35	34.53105764984399	36.0	36.0	36.0	32.0	36.0
36	34.50626307673373	36.0	36.0	36.0	32.0	36.0
37	34.457370391016966	36.0	36.0	36.0	32.0	36.0
38	34.395957343604074	36.0	36.0	36.0	32.0	36.0
39	34.32685137590768	36.0	36.0	36.0	32.0	36.0
40	34.29068176388396	36.0	36.0	36.0	32.0	36.0
41	34.2585808712272	36.0	36.0	36.0	32.0	36.0
42	34.1758613449941	36.0	36.0	36.0	32.0	36.0
43	34.18325454111072	36.0	36.0	36.0	32.0	36.0
44	34.16134427012822	36.0	36.0	36.0	32.0	36.0
45	34.03965046659958	36.0	36.0	36.0	32.0	36.0
46	34.11569642430517	36.0	36.0	36.0	32.0	36.0
47	34.04840762486697	36.0	36.0	36.0	32.0	36.0
48	34.03480275398727	36.0	36.0	36.0	32.0	36.0
49	34.03769574377203	36.0	36.0	36.0	32.0	36.0
50	33.522338138090305	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	6.0
21	23.0
22	55.0
23	223.0
24	556.0
25	1292.0
26	2913.0
27	5577.0
28	9055.0
29	13292.0
30	18489.0
31	25641.0
32	37926.0
33	57247.0
34	123015.0
35	395321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49864745100992	18.161952536246577	11.411371838018502	24.928028174725
2	16.996275788571364	19.593377506458005	36.40691788200679	27.003428822963848
3	19.309125554564517	23.713062817824834	28.584832443327272	28.39297918428338
4	12.947831916517167	14.676246284205938	36.183182674445035	36.19273912483186
5	13.79628892251334	37.28901662962346	33.579387085797855	15.335307362065345
6	33.63585817048732	36.21899940923676	16.245120411449225	13.9000220088267
7	30.061465173354957	30.75633293997553	20.44480803897862	18.737393847690893
8	29.260752785480033	32.455638651386046	18.795166766816045	19.488441796317883
9	27.016296596610367	13.82988119629037	18.97138140986194	40.18244079723733
10	16.197267731869946	26.89684131270497	30.977578605196666	25.92831235022841
11	37.353594880074134	22.06824154582377	20.470291832878438	20.107871741223658
12	24.15617511420649	22.944391755413495	30.213354376769203	22.686078753610808
13	29.816907628486828	20.24137207063065	25.25675646325483	24.68496383762769
14	23.622463385145554	19.964525400537187	25.20463052118702	31.208380693130234
15	26.051677079788888	27.431132218899997	22.34378506736554	24.17340563394557
16	26.842109073533777	26.012727417521557	23.572654151614095	23.572509357330574
17	24.01109124211776	25.54315955606073	26.38817899469329	24.057570207128222
18	25.314963765230548	25.320031565153805	25.829417854583102	23.53558681503254
19	26.00809400044886	26.132761878561034	23.901481969491844	23.957662151498262
20	25.70938339354362	24.09188645232286	25.955968058381057	24.24276209575246
21	26.417572234248194	24.544658176895176	23.822569084972525	25.215200503884105
22	23.909156066518495	25.05259652348925	26.29724818464167	24.74099922535058
23	23.936666980387614	24.65267471240235	25.37346065577331	26.03719765143672
24	24.24869866137685	26.78129547445467	24.54581653116335	24.424189333005135
25	26.34879494957539	24.388569939258797	24.98743909590449	24.275196015261315
26	23.639549110601113	24.055253498591874	25.54938571025216	26.755811680554853
27	24.627625301353103	25.62352038341526	25.528535333425033	24.2203189818066
28	23.88830568969137	25.019149043995743	25.526942596306295	25.565602670006587
29	23.953028734425565	26.163313472384107	25.466852968644798	24.416804824545526
30	25.44064520332737	25.733419244608225	25.989849920725128	22.836085631339277
31	25.53635422473521	23.949264083054	24.122148457578895	26.392233234631895
32	24.57854003923925	25.08937427150376	25.344646593352493	24.98743909590449
33	23.815039782229398	24.5074460460301	26.488521433173815	25.18899273856668
34	23.487080730052778	26.403382394463065	26.803883382684052	23.305653492800104
35	25.240539503500404	25.11254135686723	25.681293302540414	23.965625837091952
36	24.939367393775296	25.678252622586463	24.641670346854706	24.74070963678354
37	26.22876048853591	24.595336176127766	23.89496622673337	25.280937108602952
38	25.44716094608585	25.511883990820046	24.32790113446321	24.7130539286309
39	24.90809182853461	24.120410926176636	25.779898209618686	25.19159903567007
40	25.058677883397163	25.51289755080469	25.288755999913125	24.13966856588502
41	23.963164334272083	25.7279170618344	25.908909916236507	24.40000868765701
42	26.081215113627316	25.292086268434122	24.85408356078102	23.77261505715754
43	25.229679932236277	24.29517762638731	25.167852773172516	25.307289668203897
44	24.597797678947636	24.924887965423125	26.636790779500025	23.840523576129215
45	24.90215526291022	24.93125891389808	25.060849797649986	25.105736025541713
46	25.36346985021031	23.902350735192975	25.67390879408081	25.0602706205159
47	24.941249719461076	24.048448167266358	26.380215309099597	24.630086804172972
48	25.676804679751243	25.248647983377616	24.58273907346138	24.491808263409762
49	24.150817725716188	25.19348136135585	25.938737538641977	24.71696337428598
50	23.84877685028995	26.03444656004981	24.151252108566755	25.96552448109349
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	9.5
2	9.0
3	61.0
4	113.0
5	117.5
6	122.0
7	113.0
8	104.0
9	136.0
10	168.0
11	274.0
12	380.0
13	638.0
14	896.0
15	1218.5
16	1541.0
17	1779.5
18	2018.0
19	2121.0
20	2224.0
21	2428.0
22	2632.0
23	2831.0
24	3030.0
25	3286.0
26	3542.0
27	4300.5
28	5059.0
29	6012.5
30	6966.0
31	7832.0
32	8698.0
33	10068.0
34	11438.0
35	13497.0
36	15556.0
37	19685.0
38	23814.0
39	28393.0
40	32972.0
41	35233.0
42	37494.0
43	39587.5
44	41681.0
45	47988.0
46	54295.0
47	58934.5
48	63574.0
49	62111.0
50	60648.0
51	58709.5
52	56771.0
53	50790.5
54	44810.0
55	43290.0
56	41770.0
57	39839.5
58	37909.0
59	34976.0
60	32043.0
61	29946.0
62	27849.0
63	26249.5
64	24650.0
65	20173.5
66	15697.0
67	14105.5
68	12514.0
69	9861.0
70	7208.0
71	5908.5
72	4609.0
73	3546.0
74	2483.0
75	1809.5
76	1136.0
77	966.5
78	797.0
79	686.0
80	575.0
81	462.5
82	350.0
83	296.5
84	243.0
85	187.5
86	132.0
87	98.0
88	64.0
89	42.5
90	21.0
91	15.5
92	10.0
93	7.0
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01259710266638673
2	0.0027510913869120447
3	4.343828505650597E-4
4	2.895885670433731E-4
5	0.0
6	4.343828505650597E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	690635.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.746946996200116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03771010618316	41.631633264481074
2	11.255225765709516	11.423366907254536
3	2.8961109217103425	4.40906362317453
4	1.1977371182456473	2.4312600821997337
5	0.6011260609830898	1.525265617737171
6	0.3617983080706876	1.101609573778684
7	0.22734401475249427	0.8075910266583713
8	0.18432585506617147	0.7483179517657815
9	0.13060263801264346	0.5964916633912376
>10	0.8229171332861157	8.411320542306044
>50	0.12951372163658922	4.596481140183892
>100	0.13782320331724432	14.428071654752959
>500	0.013180597406637832	4.6257073870595615
>1k	0.004584555619700115	3.2638195652564725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTATAACCCTGGAATCGGATTATCCGGAGATAGGGTCTAATGGCTGGT	1859	0.26917257306681536	No Hit
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA	1847	0.26743504166455506	No Hit
ACATTATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGAT	1811	0.26222244745777434	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1704	0.2467294591209539	No Hit
TCCGAAGACTGGCCAAGGGCGCCTGCTCGGGTCCAAAACAGGTCCGCCGC	1663	0.24079289349656477	No Hit
ACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGC	1586	0.22964373366539487	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1574	0.22790620226313466	No Hit
GGGTAGGGTACGTCGGGGCCTTGGGCAAATGTGAGGGACCGAGATGGGAC	1307	0.18924612856284434	No Hit
ATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGG	1187	0.17187081454024195	No Hit
GTTCATAGTTGGTAGTTAAGCGTACGCCAGACGTCCGAAGACTGGCCAAG	1179	0.17071246027206846	No Hit
GTCCACAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACATGCG	1169	0.16926451743685159	No Hit
ATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCA	1144	0.16564466034880942	No Hit
GTCCGTACCAGTTCATAGTTGGTAGTTAAGCGTACGCCAGACGTCCGAAG	1126	0.1630383632454191	No Hit
GTTATAAGCTGTAAAGGAGAAAAGATAACTCTTCCCAGGGCCCACACCGG	1123	0.162603980394854	No Hit
CCATAGAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC	1085	0.15710179762102994	No Hit
GTCCTAAGAGATAGGGGAACTCCGTTTCAAAGTGTGCGCTTGTTCGCACG	1082	0.15666741477046486	No Hit
GTCTAATGGCTGGTAGAGCATTGCTATTTTGTAATGTCCGGTGCGTCCAC	983	0.1423327807018179	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	947	0.13712018649503718	No Hit
GGCCAAGGGCGCCTGCTCGGGTCCAAAACAGGTCCGCCGCAGCTTGCGCC	945	0.1368305979279938	No Hit
CCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAAGCGTACGCCAGACGT	933	0.13509306652573358	No Hit
GACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCG	903	0.13074923802008298	No Hit
ATTTTGTAATGTCCGGTGCGTCCACAACGGCCCTTGAAAATCCGAGGGAA	865	0.12524705524625887	No Hit
GGGTACGATCGCATGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCC	860	0.12452308382865043	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCATAGTTGG	856	0.12394390669456369	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT	843	0.12206158100878178	No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA	827	0.11974487247243479	No Hit
CCTTGGGGACCTGATGCGGATATGGGTACGATCGCATGTAAGACTTTTTC	821	0.11887610677130467	No Hit
GTTGTGGACGCACCGGACATTACAAAATAGCAATGCTCTACCAGCCATTA	791	0.11453227826565407	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	771	0.11163639259522033	No Hit
GCCTATCGAAAGGGAATACGGTTAAAATTCCGTAACCGCAATGTAGATTC	758	0.10975406690943842	No Hit
GACGTACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTAT	758	0.10975406690943842	No Hit
GTTTCAAAGTGTGCGCTTGTTCGCACGGCCTATCGAAAGGGAATACGGTT	740	0.10714776980604807	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAAGCGTACG	733	0.10613420982139624	No Hit
GTCCCATCTCGGTCCCTCACATTTGCCCAAGGCCCCGACGTACCCTACCC	729	0.10555503268730951	No Hit
CTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC	721	0.10439667841913602	No Hit
AGATGGGACTACTGCGGCGCAAGCTGCGGCGGACCTGTTTTGGACCCGAG	710	0.10280394130039747	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	707	0.10236955844983239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.4479428352168655E-4
7	0.0	0.0	0.0	0.0	1.4479428352168655E-4
8	0.0	0.0	0.0	0.0	1.4479428352168655E-4
9	0.0	0.0	0.0	0.0	1.4479428352168655E-4
10	0.0	0.0	0.0	1.4479428352168655E-4	1.4479428352168655E-4
11	0.0	0.0	0.0	1.4479428352168655E-4	1.4479428352168655E-4
12	0.0	0.0	0.0	1.4479428352168655E-4	2.895885670433731E-4
13	0.0	0.0	0.0	1.4479428352168655E-4	2.895885670433731E-4
14	0.0	0.0	0.0	1.4479428352168655E-4	2.895885670433731E-4
15	0.0	0.0	0.0	1.4479428352168655E-4	4.343828505650597E-4
16	0.0	0.0	0.0	4.343828505650597E-4	4.343828505650597E-4
17	0.0	0.0	0.0	5.791771340867462E-4	4.343828505650597E-4
18	0.0	0.0	0.0	7.239714176084329E-4	4.343828505650597E-4
19	0.0	0.0	0.0	8.687657011301194E-4	4.343828505650597E-4
20	0.0	0.0	0.0	0.0011583542681734924	7.239714176084329E-4
21	0.0	0.0	0.0	0.0011583542681734924	7.239714176084329E-4
22	0.0	0.0	0.0	0.0024615028198686716	8.687657011301194E-4
23	0.0	0.0	0.0	0.0040542399386072235	8.687657011301194E-4
24	0.0	0.0	0.0	0.007094919892562642	8.687657011301194E-4
25	0.0	0.0	0.0	0.007963685593692761	8.687657011301194E-4
26	0.0	0.0	0.0	0.010280394130039746	8.687657011301194E-4
27	0.0	0.0	0.0	0.013176279800473477	8.687657011301194E-4
28	0.0	0.0	0.0	0.020705582543601178	8.687657011301194E-4
29	0.0	0.0	0.0	0.030262005256032492	8.687657011301194E-4
30	0.0	0.0	0.0	0.052415530634850535	8.687657011301194E-4
31	0.0	0.0	0.0	0.08904848436583723	8.687657011301194E-4
32	0.0	0.0	0.0	0.14088483786660103	8.687657011301194E-4
33	0.0	0.0	0.0	0.18866695142875758	8.687657011301194E-4
34	0.0	0.0	0.0	0.24658466483743222	8.687657011301194E-4
35	0.0	0.0	0.0	0.30985976673640925	8.687657011301194E-4
36	0.0	0.0	0.0	0.3976051025505513	0.0010135599846518059
37	0.0	0.0	0.0	0.5313950205245896	0.0010135599846518059
38	0.0	0.0	0.0	0.693854206635922	0.0010135599846518059
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGATCG	30	0.005743423	29.333237	5
TAGGACG	215	0.0	26.604563	4
GTAGGAC	275	0.0	24.799917	3
CTATAGA	45	0.0013964558	24.447903	1
CGTGGAA	160	0.0	21.999928	9
CGGTTAT	60	2.8711156E-4	21.999928	43
ACGTGGA	160	0.0	21.999928	8
GAATATG	365	0.0	21.095821	13
AGCGATT	115	8.754796E-9	21.04341	26
GTTCTAA	75	5.4623273E-5	20.53624	1
TAGAGTG	150	2.1827873E-11	20.533264	5
GGACGTG	290	0.0	20.48269	6
GCGATTT	120	1.4288162E-8	20.1666	27
ACGCACC	295	0.0	20.135527	8
GACGTGG	175	0.0	20.11422	7
CTGGTCG	55	0.004481783	19.999935	9
CGAACGT	100	1.1220436E-6	19.799934	4
GGCGTTA	100	1.1220436E-6	19.799934	42
TAACACT	100	1.1220436E-6	19.799934	4
CTGTAGG	315	0.0	19.558323	1
>>END_MODULE