FastQCFastQC Report
Thu 2 Feb 2017
SRR4062591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47437
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1590.33518139848641354No Hit
TATCAACGCAGAGTACTTTTTTTTT1170.2466429158673609No Hit
GGACTCTTTCGAGGCCCTGTAATTG610.12859160570862407No Hit
GGTATCAACGCAGAGTACTTTTTTT600.1264835465986466No Hit
ACGCAGAGTACTTTTTTTTTTTTTT480.1011868372789173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTA250.005948100719.02
GTATCAA801.201206E-714.251
ATCAACG1105.6551853E-610.3636363
AACGCAG1053.8812257E-59.952386
TCAACGC1053.8812257E-59.952384
CAACGCA1053.8812257E-59.952385
TATCAAC1201.5923402E-59.4999992
CAGAGTA1252.598829E-48.3610
AGAGTAC1252.598829E-48.3611
ACGCAGA1303.9671865E-48.0384627
GCAGAGT1303.9671865E-48.0384629
TACATGG1701.3342131E-57.82352972
GTACATG1751.9637517E-57.61
CGCAGAG1408.7765924E-47.4642868
ACATGGG1802.8551678E-57.3888893
Produced by FastQC (version 0.11.5)