##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062591_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47437
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.249088264434935	32.0	32.0	32.0	14.0	32.0
2	27.935366907688092	32.0	32.0	32.0	14.0	32.0
3	28.2337626747054	32.0	32.0	32.0	14.0	32.0
4	27.779665661825156	32.0	21.0	32.0	14.0	32.0
5	27.834812488142166	32.0	21.0	32.0	14.0	32.0
6	31.238800935978244	36.0	32.0	36.0	14.0	36.0
7	31.17195438160086	36.0	32.0	36.0	14.0	36.0
8	30.964373801041383	36.0	32.0	36.0	14.0	36.0
9	31.328583173472182	36.0	32.0	36.0	14.0	36.0
10	30.241457090456816	36.0	21.0	36.0	14.0	36.0
11	31.720724329110187	36.0	32.0	36.0	14.0	36.0
12	30.78881463836246	36.0	32.0	36.0	14.0	36.0
13	31.203090414655225	36.0	32.0	36.0	14.0	36.0
14	30.786242806248286	36.0	32.0	36.0	14.0	36.0
15	30.659337647827645	36.0	27.0	36.0	14.0	36.0
16	30.759112085502878	36.0	32.0	36.0	14.0	36.0
17	30.52924932015094	36.0	27.0	36.0	14.0	36.0
18	30.62683137635179	36.0	27.0	36.0	14.0	36.0
19	30.610683643569367	36.0	27.0	36.0	14.0	36.0
20	30.530493075025824	36.0	27.0	36.0	14.0	36.0
21	30.562767459999577	36.0	27.0	36.0	14.0	36.0
22	30.41703733372684	36.0	27.0	36.0	14.0	36.0
23	30.37923983388494	36.0	27.0	36.0	14.0	36.0
24	30.313742437337943	36.0	27.0	36.0	14.0	36.0
25	29.751818200982356	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	6.0
6	7.0
7	4.0
8	9.0
9	5.0
10	3.0
11	2.0
12	1.0
13	0.0
14	40.0
15	105.0
16	250.0
17	559.0
18	906.0
19	1103.0
20	1274.0
21	1269.0
22	1308.0
23	1236.0
24	1309.0
25	1371.0
26	1417.0
27	1517.0
28	1772.0
29	1917.0
30	2280.0
31	2665.0
32	3483.0
33	4347.0
34	7703.0
35	9563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04245989079333	19.022621381738453	11.721797061117787	27.213121666350432
2	16.725693566067964	20.290496669196393	37.79408044523147	25.189729319504174
3	18.19964161484136	24.450300411088858	29.01233266575314	28.337725308316642
4	12.303118476817003	15.771606890589748	36.20722373331646	35.71805089927678
5	14.216420271210747	36.349832338612735	34.00257291688635	15.431174473290172
6	32.696404091532216	36.74997363703469	16.80269956764737	13.750922703785722
7	28.757356508532496	31.060813803868626	21.48416900457738	18.697660683021493
8	27.290563490221725	32.83053100145567	20.149363937469676	19.729541570852934
9	26.832355795603192	14.00059074222541	19.23287902443141	39.93417443773999
10	15.145504041192732	26.66132061535864	32.16282946799755	26.030345875451072
11	36.32910591065437	21.48599886049505	23.0707548165186	19.114140412331977
12	23.642658110276113	24.26749978890484	29.428776492442793	22.66106560837626
13	28.61092351848725	19.93288873881479	25.939135573189265	25.5170521695087
14	22.78291334261956	19.720568992444388	25.959225022160314	31.53729264277574
15	24.28731193686565	27.46935072060096	23.008588128547615	25.23474921398578
16	24.94196598151353	26.372768328198205	24.389060059933314	24.296205630354958
17	23.29463908822288	26.418319966230474	25.812579147319543	24.4744617982271
18	23.71353792899658	25.129807083456456	27.3544683186289	23.80218666891806
19	24.779452112616607	25.598328479169304	26.339116120045585	23.2831032881685
20	24.5605149308853	24.459217051809645	26.37332489184341	24.606943125461644
21	25.59567777472933	24.72405715129899	25.63577654433025	24.044488529641434
22	24.358270708435363	24.867010048129696	26.156801486109938	24.617917757325003
23	23.399045970703703	24.6844526995652	26.6621638735278	25.254337456203302
24	23.332629801604053	25.745040101308568	26.439425918108906	24.482904178978472
25	24.04229722028747	24.983642542054497	26.21414550750332	24.759914730154712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	16.5
3	29.0
4	29.0
5	29.0
6	64.0
7	99.0
8	99.0
9	99.0
10	130.5
11	162.0
12	162.0
13	162.0
14	199.5
15	237.0
16	237.0
17	237.0
18	357.0
19	477.0
20	477.0
21	477.0
22	742.0
23	1007.0
24	1007.0
25	1007.0
26	1445.5
27	1884.0
28	1884.0
29	1884.0
30	2248.5
31	2613.0
32	2613.0
33	2613.0
34	3063.0
35	3513.0
36	3513.0
37	3513.0
38	3757.0
39	4001.0
40	4001.0
41	4001.0
42	4426.5
43	4852.0
44	4852.0
45	4852.0
46	5337.5
47	5823.0
48	5823.0
49	5823.0
50	5828.0
51	5833.0
52	5833.0
53	5833.0
54	5336.0
55	4839.0
56	4839.0
57	4839.0
58	4395.0
59	3951.0
60	3951.0
61	3951.0
62	3533.5
63	3116.0
64	3116.0
65	3116.0
66	2679.5
67	2243.0
68	2243.0
69	2243.0
70	1700.0
71	1157.0
72	1157.0
73	1157.0
74	925.0
75	693.0
76	693.0
77	693.0
78	590.0
79	487.0
80	487.0
81	487.0
82	353.0
83	219.0
84	219.0
85	219.0
86	163.0
87	107.0
88	107.0
89	107.0
90	69.5
91	32.0
92	32.0
93	32.0
94	21.0
95	10.0
96	10.0
97	10.0
98	29.5
99	49.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008432236439909774
2	0.0021080591099774436
3	0.004216118219954887
4	0.021080591099774438
5	0.042161182199548876
6	0.04637730041950376
7	0.0632417732993233
8	0.07589012795918798
9	0.0822143052891203
10	0.10540295549887219
11	0.1011868372789173
12	0.13702384214853383
13	0.11172713282880452
14	0.1159432510487594
15	0.09697071905896242
16	0.10751101460884964
17	0.1201593692687143
18	0.12437548748866918
19	0.1159432510487594
20	0.10961907371882709
21	0.11383519193878197
22	0.13702384214853383
23	0.12437548748866918
24	0.1201593692687143
25	0.12226742837869173
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	47437.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.63271286126863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.4640090078014	72.70695870312204
2	3.895337926597142	6.126019773594452
3	1.2627007318838637	2.9786875223981277
4	0.5844347336532533	1.8382275439003308
5	0.34851612557304096	1.3702384214853385
6	0.2466421811747674	1.163648628707549
7	0.18498163588107558	1.0181925501191054
8	0.15281091659741028	0.9612749541497144
9	0.1260019838610225	0.8917090035204587
>10	0.7238411838824697	10.108143432341842
>50	0.0053617865472775535	0.25507515230727074
>100	0.0053617865472775535	0.5818243143537745
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	159	0.33518139848641354	No Hit
TATCAACGCAGAGTACTTTTTTTTT	117	0.2466429158673609	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	61	0.12859160570862407	No Hit
GGTATCAACGCAGAGTACTTTTTTT	60	0.1264835465986466	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	48	0.1011868372789173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTGTA	25	0.0059481007	19.0	2
GTATCAA	80	1.201206E-7	14.25	1
ATCAACG	110	5.6551853E-6	10.363636	3
AACGCAG	105	3.8812257E-5	9.95238	6
TCAACGC	105	3.8812257E-5	9.95238	4
CAACGCA	105	3.8812257E-5	9.95238	5
TATCAAC	120	1.5923402E-5	9.499999	2
CAGAGTA	125	2.598829E-4	8.36	10
AGAGTAC	125	2.598829E-4	8.36	11
ACGCAGA	130	3.9671865E-4	8.038462	7
GCAGAGT	130	3.9671865E-4	8.038462	9
TACATGG	170	1.3342131E-5	7.8235297	2
GTACATG	175	1.9637517E-5	7.6	1
CGCAGAG	140	8.7765924E-4	7.464286	8
ACATGGG	180	2.8551678E-5	7.388889	3
>>END_MODULE