FastQCFastQC Report
Thu 2 Feb 2017
SRR4062591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47437
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC810.17075278790817294No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT610.12859160570862407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500.10540295549887219No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG480.1011868372789173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTG250.002330407335.1991585
CAAGACT250.002330407335.1991584
TCTTATA500.00254473321.99947444
ACATGGG1251.6185777E-414.0796623
TACATGG1250.002601157612.3197042
GTACATG1300.003462144211.8583791
Produced by FastQC (version 0.11.5)