FastQCFastQC Report
Thu 2 Feb 2017
SRR4062590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119734
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29330.2619372100873958No Hit
GTACATGGAAGCAGTGGTATCAACG24450.21835543084339676No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19930.17798870088788946No Hit
TATCAACGCAGAGTACTTTTTTTTT19290.17227305770834858No Hit
TCCATGTACTCTGCGTTGATACCAC18950.1692366222692175No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15870.14173008946767715No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13540.1209215760171612No Hit
GAATAGGACCGCGGTTCTATTTTGT12740.11377702204273515No Hit
GCGCAAGACGGACCAGAGCGAAAGC12650.11297325972061223No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12330.11011543813084178No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12310.10993682428148113No Hit
GAACTACGACGGTATCTGATCGTCT12170.10868652733595657No Hit
GATTAAGAGGGACGGCCGGGGGCAT11880.10609662652022711No Hit
GAATAACGCCGCCGCATCGCCAGTC11590.10350672570449769No Hit
GTCCTATTCCATTATTCCTAGCTGC11410.1018992010602518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT807.759809E-915.4293681
CTATTAT801.2956298E-714.2424941
TAGACCT903.6452548E-813.7192854
CGTTATT957.417475E-812.9948922
CGCAAGA2300.012.7993182
CGGTTCT2900.012.12080412
ACTCTAA2200.012.09156410
GGCAGTA550.003067304112.0910237
CTTATAC1103.8276994E-812.08669953
TCTAGAT2450.012.0156862
CAAGACG2800.011.87245754
AAGACGG2900.011.7916345
GTATTAG2500.011.7737941
TTCGGAA1302.5956979E-911.69660118
TTACCGC1950.011.694509516
CGGAATT658.013687E-411.69346315
TTACACC658.034755E-411.6898044
TAGCACC907.473007E-611.6086254
ATAGTAC907.484172E-611.6070693
CGGTCCA2050.011.58599310
Produced by FastQC (version 0.11.5)