##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062590_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1119734
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12147527895018	32.0	32.0	32.0	32.0	32.0
2	30.75906152711269	32.0	32.0	32.0	32.0	32.0
3	30.747063141781886	32.0	32.0	32.0	32.0	32.0
4	30.830793742085174	32.0	32.0	32.0	32.0	32.0
5	30.60580191366878	32.0	32.0	32.0	32.0	32.0
6	34.247660605107995	36.0	36.0	36.0	32.0	36.0
7	34.120254453289796	36.0	36.0	36.0	32.0	36.0
8	34.10895534117925	36.0	36.0	36.0	32.0	36.0
9	34.278659931733785	36.0	36.0	36.0	32.0	36.0
10	33.922843282422434	36.0	36.0	36.0	32.0	36.0
11	34.27239147869047	36.0	36.0	36.0	32.0	36.0
12	34.03190847111903	36.0	36.0	36.0	32.0	36.0
13	34.17672679404215	36.0	36.0	36.0	32.0	36.0
14	34.076861111656875	36.0	36.0	36.0	32.0	36.0
15	34.00312306315607	36.0	36.0	36.0	32.0	36.0
16	33.998079901119375	36.0	36.0	36.0	32.0	36.0
17	33.92405696352884	36.0	36.0	36.0	32.0	36.0
18	33.938031711102816	36.0	36.0	36.0	32.0	36.0
19	33.93456749549446	36.0	36.0	36.0	32.0	36.0
20	33.914492191895576	36.0	36.0	36.0	32.0	36.0
21	33.88602739579221	36.0	36.0	36.0	32.0	36.0
22	33.85045912689978	36.0	36.0	36.0	32.0	36.0
23	33.807539111967664	36.0	36.0	36.0	32.0	36.0
24	33.765561285090925	36.0	36.0	36.0	32.0	36.0
25	33.31546688767153	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	46.0
5	106.0
6	190.0
7	46.0
8	180.0
9	199.0
10	129.0
11	45.0
12	96.0
13	63.0
14	262.0
15	363.0
16	471.0
17	648.0
18	835.0
19	1191.0
20	1724.0
21	2531.0
22	3668.0
23	5260.0
24	7142.0
25	10292.0
26	14055.0
27	18328.0
28	24127.0
29	32331.0
30	41733.0
31	57262.0
32	81580.0
33	116826.0
34	254945.0
35	443052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.177528174184246	17.3353625533876	10.65313734211471	25.83397193031344
2	16.90920230276378	20.305385916559644	36.823768551877386	25.96164322879919
3	19.53697360935341	24.15785981135631	28.073810572156077	28.231356007134202
4	12.604170574458848	15.096672079517095	35.90765284156783	36.39150450445622
5	14.49100565971152	36.68699159753957	33.49727288564881	15.3247298571001
6	34.03189778413152	35.328895639742676	16.947373123659755	13.691833452466048
7	29.930936239349542	30.54141055224198	20.995812205196586	18.531841003211895
8	28.175578257500117	32.737906357905125	19.84940321734366	19.237112167251098
9	27.487900421406824	14.075202434397669	18.740892910460435	39.696004233735074
10	15.833733459585462	26.649928424343056	31.73533190807915	25.78100620799233
11	37.471923574897886	21.24696881907337	22.21256536263296	19.068542243395783
12	24.5108822987593	23.88266183048689	28.93230242578056	22.674153444973253
13	29.50858810621729	19.504734210876926	25.640475379331495	25.346202303574287
14	23.33967015161866	19.877907455031245	25.23588701745099	31.54653537589911
15	25.053489538581758	27.43447810930016	22.702810961309606	24.80922139080847
16	25.507683311830608	25.84403775538395	24.308627107020975	24.339651825764463
17	23.62958088030159	26.033760631791292	25.709262981694366	24.627395506212757
18	24.64125165546924	24.763494812937566	26.94044245405598	23.654811077537214
19	25.132683219093867	25.460959611541096	25.71751776189007	23.688839407474973
20	25.213411935331287	24.463754172340813	25.76725022063222	24.55558367169568
21	26.33151836149982	24.3885536054569	24.668981508390928	24.61094652465235
22	25.307252326585004	24.956464574640783	25.25081503146088	24.485468067313327
23	23.89909208634123	24.65980067395955	25.81570926730151	25.625397972397707
24	24.243234517931736	25.634114430792703	25.661032379340966	24.461618671934595
25	24.61885288781143	24.95624934608757	25.584631247043422	24.84026651905758
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	319.0
1	319.0
2	592.5
3	866.0
4	866.0
5	866.0
6	2131.0
7	3396.0
8	3396.0
9	3396.0
10	3515.5
11	3635.0
12	3635.0
13	3635.0
14	3708.0
15	3781.0
16	3781.0
17	3781.0
18	6012.0
19	8243.0
20	8243.0
21	8243.0
22	13044.0
23	17845.0
24	17845.0
25	17845.0
26	26220.5
27	34596.0
28	34596.0
29	34596.0
30	42711.5
31	50827.0
32	50827.0
33	50827.0
34	61651.0
35	72475.0
36	72475.0
37	72475.0
38	81442.0
39	90409.0
40	90409.0
41	90409.0
42	102942.0
43	115475.0
44	115475.0
45	115475.0
46	132836.5
47	150198.0
48	150198.0
49	150198.0
50	149097.0
51	147996.0
52	147996.0
53	147996.0
54	135541.0
55	123086.0
56	123086.0
57	123086.0
58	112681.0
59	102276.0
60	102276.0
61	102276.0
62	91001.5
63	79727.0
64	79727.0
65	79727.0
66	66653.0
67	53579.0
68	53579.0
69	53579.0
70	40628.5
71	27678.0
72	27678.0
73	27678.0
74	21660.5
75	15643.0
76	15643.0
77	15643.0
78	12952.0
79	10261.0
80	10261.0
81	10261.0
82	7188.0
83	4115.0
84	4115.0
85	4115.0
86	3046.0
87	1977.0
88	1977.0
89	1977.0
90	1322.0
91	667.0
92	667.0
93	667.0
94	396.0
95	125.0
96	125.0
97	125.0
98	332.0
99	539.0
100	539.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00821623707058998
2	0.003215049288491731
3	0.005090494706778574
4	0.015718018743737353
5	0.037419601441056535
6	0.04768989777929401
7	0.06858771815449026
8	0.08796732081012097
9	0.09823761714835846
10	0.11940335829759567
11	0.12127880371588252
12	0.1336031593217675
13	0.12369009068225131
14	0.12520830840181688
15	0.11529523976230069
16	0.11350910126869418
17	0.12413662530565295
18	0.13137048620475933
19	0.1311918723553987
20	0.12118949679120221
21	0.1288698923137102
22	0.14994632653826712
23	0.1398546440493903
24	0.13583583243877562
25	0.1308346446566774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1119734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.79179828151115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.36179185341877	44.03167088773307
2	12.337182847534587	13.519528678884688
3	3.3148391174942877	5.448779887842284
4	1.2642493386758675	2.7708197896904805
5	0.6830224934804604	1.8712015342258077
6	0.39630483477382766	1.3028552738949104
7	0.28399849025187934	1.089255159309428
8	0.18427911183883663	0.8077587138695657
9	0.1487817288507314	0.7336816627647386
>10	0.8277441916366625	8.564362772905806
>50	0.08903391991034053	3.4678147566677677
>100	0.09815618042910068	11.123560210217748
>500	0.007839427720416294	2.9434872723455516
>1k	0.002776463984314104	2.3252233996481566
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2933	0.2619372100873958	No Hit
GTACATGGAAGCAGTGGTATCAACG	2445	0.21835543084339676	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1993	0.17798870088788946	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1929	0.17227305770834858	No Hit
TCCATGTACTCTGCGTTGATACCAC	1895	0.1692366222692175	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1587	0.14173008946767715	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1354	0.1209215760171612	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1274	0.11377702204273515	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1265	0.11297325972061223	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1233	0.11011543813084178	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1231	0.10993682428148113	No Hit
GAACTACGACGGTATCTGATCGTCT	1217	0.10868652733595657	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1188	0.10609662652022711	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1159	0.10350672570449769	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1141	0.1018992010602518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.930692468032587E-5
7	0.0	0.0	0.0	8.930692468032587E-5	8.930692468032587E-5
8	0.0	0.0	0.0	8.930692468032587E-5	8.930692468032587E-5
9	0.0	0.0	0.0	8.930692468032587E-5	8.930692468032587E-5
10	0.0	0.0	0.0	8.930692468032587E-5	8.930692468032587E-5
11	0.0	0.0	0.0	1.7861384936065174E-4	8.930692468032587E-5
12	0.0	0.0	0.0	1.7861384936065174E-4	3.5722769872130347E-4
13	0.0	0.0	0.0	1.7861384936065174E-4	4.465346234016293E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	80	7.759809E-9	15.429368	1
CTATTAT	80	1.2956298E-7	14.242494	1
TAGACCT	90	3.6452548E-8	13.719285	4
CGTTATT	95	7.417475E-8	12.994892	2
CGCAAGA	230	0.0	12.799318	2
CGGTTCT	290	0.0	12.120804	12
ACTCTAA	220	0.0	12.091564	10
GGCAGTA	55	0.0030673041	12.091023	7
CTTATAC	110	3.8276994E-8	12.0866995	3
TCTAGAT	245	0.0	12.015686	2
CAAGACG	280	0.0	11.8724575	4
AAGACGG	290	0.0	11.791634	5
GTATTAG	250	0.0	11.773794	1
TTCGGAA	130	2.5956979E-9	11.696601	18
TTACCGC	195	0.0	11.6945095	16
CGGAATT	65	8.013687E-4	11.693463	15
TTACACC	65	8.034755E-4	11.689804	4
TAGCACC	90	7.473007E-6	11.608625	4
ATAGTAC	90	7.484172E-6	11.607069	3
CGGTCCA	205	0.0	11.585993	10
>>END_MODULE