FastQCFastQC Report
Thu 2 Feb 2017
SRR4062590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119734
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14330.12797682306690697No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12800.11431286359081712No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12450.11118712122700569No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12280.10966890350744016No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11540.10306019108109604No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11480.10252434953301409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA450.001398198524.4442967
TAGGACG3200.021.9998664
ACCGTAT550.004483567619.9998788
TGTAGGA4250.019.6713472
GTAGGAC4600.019.1303183
CGTCTTA1504.656613E-1019.06655115
GGACGTG3800.018.5262036
GTATAGG1557.185008E-1018.4556241
GGACCGT851.4307804E-418.1175376
GTACCGT750.001290842417.5998946
CGGATTT1002.4828782E-517.59989435
GATTGCG750.001290842417.59989410
AGGACGT3900.017.4870725
TACGCTC902.2152899E-417.11100834
TAGTACA800.001987714816.5006372
ACGTATG800.001988308316.49989932
TCCTACA4250.016.0476782
GTACTAA1105.6102188E-516.0034751
GTATTAT1259.419511E-615.8434411
GTATGGT2800.015.71419053
Produced by FastQC (version 0.11.5)