##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1119734
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242862144044924	32.0	32.0	32.0	32.0	32.0
2	31.3958100763217	32.0	32.0	32.0	32.0	32.0
3	31.484325741649357	32.0	32.0	32.0	32.0	32.0
4	31.59142617800299	32.0	32.0	32.0	32.0	32.0
5	31.525211344837256	32.0	32.0	32.0	32.0	32.0
6	35.10689324428837	36.0	36.0	36.0	36.0	36.0
7	35.119727542434184	36.0	36.0	36.0	36.0	36.0
8	35.06358206502616	36.0	36.0	36.0	36.0	36.0
9	35.190728333693535	36.0	36.0	36.0	36.0	36.0
10	35.05900776434403	36.0	36.0	36.0	36.0	36.0
11	35.20223821014634	36.0	36.0	36.0	36.0	36.0
12	35.09860466860879	36.0	36.0	36.0	36.0	36.0
13	35.145717643654656	36.0	36.0	36.0	36.0	36.0
14	35.108533812494755	36.0	36.0	36.0	36.0	36.0
15	35.0700246665726	36.0	36.0	36.0	36.0	36.0
16	35.084957677448394	36.0	36.0	36.0	36.0	36.0
17	35.05593739227352	36.0	36.0	36.0	36.0	36.0
18	35.06981390223035	36.0	36.0	36.0	36.0	36.0
19	35.0484552581238	36.0	36.0	36.0	36.0	36.0
20	35.0349207936885	36.0	36.0	36.0	36.0	36.0
21	35.025096138904416	36.0	36.0	36.0	36.0	36.0
22	35.004329599708505	36.0	36.0	36.0	36.0	36.0
23	34.974291215592274	36.0	36.0	36.0	32.0	36.0
24	34.94724104117585	36.0	36.0	36.0	32.0	36.0
25	34.92485804664322	36.0	36.0	36.0	32.0	36.0
26	34.88406442958774	36.0	36.0	36.0	32.0	36.0
27	34.86556092786322	36.0	36.0	36.0	32.0	36.0
28	34.84310023630612	36.0	36.0	36.0	32.0	36.0
29	34.81761382614085	36.0	36.0	36.0	32.0	36.0
30	34.80340241521647	36.0	36.0	36.0	32.0	36.0
31	34.793597407955815	36.0	36.0	36.0	32.0	36.0
32	34.75903384196604	36.0	36.0	36.0	32.0	36.0
33	34.735413053457336	36.0	36.0	36.0	32.0	36.0
34	34.71654696561862	36.0	36.0	36.0	32.0	36.0
35	34.676257932687584	36.0	36.0	36.0	32.0	36.0
36	34.64223110131513	36.0	36.0	36.0	32.0	36.0
37	34.619781126589	36.0	36.0	36.0	32.0	36.0
38	34.57934295109374	36.0	36.0	36.0	32.0	36.0
39	34.561477993880686	36.0	36.0	36.0	32.0	36.0
40	34.53668371238169	36.0	36.0	36.0	32.0	36.0
41	34.505121752130414	36.0	36.0	36.0	32.0	36.0
42	34.46186326395376	36.0	36.0	36.0	32.0	36.0
43	34.46397805193019	36.0	36.0	36.0	32.0	36.0
44	34.419389783645045	36.0	36.0	36.0	32.0	36.0
45	34.361122373706614	36.0	36.0	36.0	32.0	36.0
46	34.36532158530508	36.0	36.0	36.0	32.0	36.0
47	34.3321699617945	36.0	36.0	36.0	32.0	36.0
48	34.27628704674503	36.0	36.0	36.0	32.0	36.0
49	34.27714528629121	36.0	36.0	36.0	32.0	36.0
50	33.743270276690716	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	9.0
21	31.0
22	102.0
23	308.0
24	849.0
25	1926.0
26	4057.0
27	7445.0
28	12346.0
29	19040.0
30	26673.0
31	37625.0
32	55794.0
33	88804.0
34	192350.0
35	672370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50624291592719	17.94899735527948	11.229745915688703	26.31501381310463
2	16.21753657061865	20.780524487734475	36.479342813992886	26.522596127653987
3	18.897530907197584	24.29958123571633	28.62708009034377	28.17580776674231
4	12.311149175741003	15.554154427885933	36.12200408490238	36.01269231147068
5	14.194442608690993	36.88920761538008	33.49974190298767	15.41660787294125
6	34.27537759170084	35.489779660774765	16.642077361644972	13.592765385879426
7	29.915051253244073	30.590122296902656	20.981590270546395	18.513236179306872
8	28.188837706098056	32.79019838640249	19.692980654333976	19.327983253165485
9	27.487510426583455	13.899729757245918	18.662646664297057	39.950113151873566
10	15.72864626777431	26.73233107148662	31.836757658515324	25.70226500222374
11	37.691719640557494	21.01213323878707	22.192234941512893	19.103912179142547
12	24.50394468686313	23.753587905699032	28.911152112912532	22.831315294525307
13	29.637663945186983	19.44488601757203	25.574377486081517	25.343072551159473
14	23.4382451546528	19.676726794042157	25.291542455618927	31.593485595686115
15	24.995400693378965	27.45312726058153	22.730130548862498	24.82134149717701
16	25.57018006062154	25.799609550125297	24.283267275978044	24.346943113275117
17	23.714114244990327	25.941339639592975	25.64135767959176	24.703188435824938
18	24.760255560695665	24.54502587221608	27.06124847508426	23.633470092003993
19	25.312350969069442	25.232242657631186	25.786481432197288	23.66892494110208
20	25.381921063395414	24.28907222608227	25.884629742420966	24.444376968101352
21	26.378586342827848	24.33122509453138	24.729712592454995	24.560475970185777
22	25.38576126115667	24.699705465762403	25.3960315574949	24.518501715586023
23	23.96658492106161	24.32291955053611	25.979116468732755	25.73137905966953
24	24.320151035871017	25.283683446247057	25.752812721592804	24.64335279628912
25	24.732481107120083	24.541007060605466	25.803806975585275	24.92270485668918
26	23.953546110058284	25.343072551159473	26.379390105149973	24.323991233632274
27	25.00808227668357	25.053092966722453	25.489803828409247	24.44902092818473
28	24.066162142080174	24.74730605661702	26.284188923440745	24.902342877862065
29	24.03999521314884	25.050413758982042	26.218191106101983	24.691399921767136
30	24.22182411179798	25.095960290569007	26.3710845611547	24.311131036478308
31	24.548240921504572	25.071490193206603	25.358076114505767	25.022192770783057
32	24.230308269642613	25.095960290569007	25.516953133512065	25.156778306276312
33	23.934434428176694	24.664965072061758	26.15112160566706	25.24947889409449
34	24.576283295854193	25.05675455063435	26.267399221600847	24.099562931910615
35	25.407820071552706	24.757397739105894	25.931069343254737	23.903712846086663
36	24.014274818840903	25.444257296822283	25.792643610000233	24.748824274336584
37	25.19759157085522	25.099979102179624	25.518649965080993	24.183779361884163
38	24.275229652756817	24.81964466560808	25.911421819825065	24.993703861810037
39	24.673181309132346	24.80580209228263	25.55964184350926	24.96137475507576
40	25.088369201971183	24.91332762959774	26.00581923921217	23.992483929218906
41	23.808868892076156	25.072472569378085	26.679104144377146	24.439554394168614
42	25.251711567211498	25.48533848217523	25.79067885765727	23.472271092956007
43	24.238613813637883	24.505552211507375	26.084677253704896	25.171156721149845
44	23.879867897197013	25.15981474171544	25.91231488907187	25.048002472015675
45	23.93050492349076	25.37450858864695	25.818364004308165	24.87662248355413
46	23.77526388034269	24.983768422951584	26.027590555231566	25.213377141474158
47	24.3417850505433	24.5788951473253	26.408795668253056	24.670524133878345
48	24.755165065988887	25.792554303075555	25.129896921947537	24.322383708988028
49	23.74396396283757	25.445351704379526	25.840177970998447	24.970506361784462
50	23.60089414172843	26.174096860590872	25.268255914579633	24.95675308310106
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	56.5
2	72.0
3	216.5
4	361.0
5	392.0
6	423.0
7	366.5
8	310.0
9	389.0
10	468.0
11	739.5
12	1011.0
13	1581.0
14	2151.0
15	2940.5
16	3730.0
17	4070.5
18	4411.0
19	4658.5
20	4906.0
21	5458.0
22	6010.0
23	6449.5
24	6889.0
25	7803.5
26	8718.0
27	10691.0
28	12664.0
29	14309.0
30	15954.0
31	17806.5
32	19659.0
33	22988.0
34	26317.0
35	29963.5
36	33610.0
37	39172.0
38	44734.0
39	47355.0
40	49976.0
41	54224.0
42	58472.0
43	59359.5
44	60247.0
45	66984.0
46	73721.0
47	78525.5
48	83330.0
49	84284.0
50	85238.0
51	80838.0
52	76438.0
53	74560.0
54	72682.0
55	73613.5
56	74545.0
57	72708.0
58	70871.0
59	64590.5
60	58310.0
61	51587.0
62	44864.0
63	39321.5
64	33779.0
65	29056.0
66	24333.0
67	21314.5
68	18296.0
69	16878.5
70	15461.0
71	12184.5
72	8908.0
73	8006.5
74	7105.0
75	5236.5
76	3368.0
77	2970.5
78	2573.0
79	2211.5
80	1850.0
81	1546.5
82	1243.0
83	1033.0
84	823.0
85	669.5
86	516.0
87	378.0
88	240.0
89	154.5
90	69.0
91	43.5
92	18.0
93	14.0
94	10.0
95	7.5
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01294950407864725
2	0.004554653158696619
3	9.823761714835846E-4
4	8.930692468032585E-5
5	0.0
6	5.358415480819552E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.679207740409776E-4
47	8.930692468032585E-5
48	0.0
49	8.930692468032585E-5
50	8.930692468032585E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1119734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.412315528085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02602786045577	47.32917905372349
2	12.089973950464321	14.124067462417006
3	3.1836662296492997	5.57895949027151
4	1.1914579418186488	2.7838326894381464
5	0.6515569238658026	1.9029474310678858
6	0.3747419191167529	1.3133725932628728
7	0.25265189428648316	1.0330587517481302
8	0.18218017316450663	0.8513252606277062
9	0.1360336251231281	0.7151435129809252
>10	0.7486857374030378	8.14892670968875
>50	0.07645140377995005	3.2016018363396035
>100	0.07826877889629971	9.327133504254558
>500	0.007073404646189541	2.825121752660337
>1k	0.0012301573297720941	0.865329951519082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1433	0.12797682306690697	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1280	0.11431286359081712	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1245	0.11118712122700569	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1228	0.10966890350744016	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1154	0.10306019108109604	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1148	0.10252434953301409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.930692468032587E-5
2	0.0	0.0	0.0	0.0	8.930692468032587E-5
3	0.0	0.0	0.0	0.0	8.930692468032587E-5
4	0.0	0.0	0.0	0.0	8.930692468032587E-5
5	0.0	0.0	0.0	0.0	8.930692468032587E-5
6	0.0	0.0	0.0	0.0	1.7861384936065174E-4
7	0.0	0.0	0.0	0.0	1.7861384936065174E-4
8	0.0	0.0	0.0	0.0	1.7861384936065174E-4
9	0.0	0.0	0.0	0.0	1.7861384936065174E-4
10	0.0	0.0	0.0	0.0	1.7861384936065174E-4
11	0.0	0.0	0.0	8.930692468032587E-5	1.7861384936065174E-4
12	0.0	0.0	0.0	8.930692468032587E-5	6.25148472762281E-4
13	0.0	0.0	0.0	8.930692468032587E-5	7.144553974426069E-4
14	0.0	0.0	0.0	8.930692468032587E-5	7.144553974426069E-4
15	0.0	0.0	0.0	8.930692468032587E-5	7.144553974426069E-4
16	0.0	0.0	0.0	3.5722769872130347E-4	8.930692468032586E-4
17	0.0	0.0	0.0	5.358415480819552E-4	8.930692468032586E-4
18	0.0	0.0	0.0	5.358415480819552E-4	0.0010716830961639103
19	0.0	0.0	0.0	8.037623221229328E-4	0.0010716830961639103
20	0.0	0.0	0.0	8.930692468032586E-4	0.001250296945524562
21	0.0	0.0	0.0	0.0010716830961639103	0.001250296945524562
22	0.0	0.0	0.0	0.0021433661923278206	0.001250296945524562
23	0.0	0.0	0.0	0.0031257423638114054	0.001339603870204888
24	0.0	0.0	0.0	0.005358415480819552	0.001339603870204888
25	0.0	0.0	0.0	0.007591088597827698	0.001339603870204888
26	0.0	0.0	0.0	0.0103596032629178	0.0014289107948852139
27	0.0	0.0	0.0	0.013842573325450509	0.0014289107948852139
28	0.0	0.0	0.0	0.022594651944122444	0.0014289107948852139
29	0.0	0.0	0.0	0.037508908365736865	0.0014289107948852139
30	0.0	0.0	0.0	0.06742672813364603	0.0014289107948852139
31	0.0	0.0	0.0	0.12976296156051348	0.0015182177195655396
32	0.0	0.0	0.0	0.20210157055157743	0.0015182177195655396
33	0.0	0.0	0.0	0.2714037441035103	0.0015182177195655396
34	0.0	0.0	0.0	0.3516013624664429	0.0017861384936065173
35	0.0	0.0	0.0	0.44224789101697365	0.0017861384936065173
36	0.0	0.0	0.0	0.5735290702970527	0.0017861384936065173
37	0.0	0.0	0.0	0.7638421267908271	0.0017861384936065173
38	0.0	0.0	0.0	1.0005054771936905	0.0018754454182868432
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	45	0.0013981985	24.444296	7
TAGGACG	320	0.0	21.999866	4
ACCGTAT	55	0.0044835676	19.999878	8
TGTAGGA	425	0.0	19.671347	2
GTAGGAC	460	0.0	19.130318	3
CGTCTTA	150	4.656613E-10	19.066551	15
GGACGTG	380	0.0	18.526203	6
GTATAGG	155	7.185008E-10	18.455624	1
GGACCGT	85	1.4307804E-4	18.117537	6
GTACCGT	75	0.0012908424	17.599894	6
CGGATTT	100	2.4828782E-5	17.599894	35
GATTGCG	75	0.0012908424	17.599894	10
AGGACGT	390	0.0	17.487072	5
TACGCTC	90	2.2152899E-4	17.111008	34
TAGTACA	80	0.0019877148	16.500637	2
ACGTATG	80	0.0019883083	16.499899	32
TCCTACA	425	0.0	16.047678	2
GTACTAA	110	5.6102188E-5	16.003475	1
GTATTAT	125	9.419511E-6	15.843441	1
GTATGGT	280	0.0	15.7141905	3
>>END_MODULE