FastQCFastQC Report
Thu 2 Feb 2017
SRR4062589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504601
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG56230.37372034180490377No Hit
TCCATGTACTCTGCGTTGATACCAC47310.31443552144389114No Hit
GTATCAACGCAGAGTACTTTTTTTT30770.20450604512425555No Hit
GAGTACATGGAAGCAGTGGTATCAA26400.17546180017160698No Hit
CATGTACTCTGCGTTGATACCACTG22170.14734803446229267No Hit
TATCAACGCAGAGTACTTTTTTTTT22030.14641755521895838No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18580.12348788815107793No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16930.11252152564035249No Hit
GCGCAAGACGGACCAGAGCGAAAGC15960.10607463374010784No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15300.10168808873581767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1550.015.3222057
TAAGACA1500.014.5619464
TTCACGA400.005300878514.2411172
TCTAGTA602.583294E-514.2411172
CGACTTT903.62852E-813.72508313
TAAGACT1001.01154E-813.2956914
CGCGTAA1251.0186341E-1012.92097410
CTAAGAC1251.03682396E-1012.9132363
GTATTAG2500.012.9089391
CGCGGTC1850.012.83880510
GAACCGC2000.012.8246866
CGAACGT1155.347829E-912.3872894
GTGTTAA1155.3914846E-912.3806971
AAGACGG3000.012.3476425
GAATAAG701.09960965E-412.203831
GTATATA1405.2750693E-1112.203831
AGAACCG2300.011.9759745
TCGCGTA1353.7107384E-1011.9634679
CGAACGA1209.958967E-911.87905916
ACTGCGC802.8659537E-511.8751048
Produced by FastQC (version 0.11.5)