##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062589_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1504601
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.095582151015453	32.0	32.0	32.0	32.0	32.0
2	30.725710005509768	32.0	32.0	32.0	32.0	32.0
3	30.716530827774275	32.0	32.0	32.0	32.0	32.0
4	30.787639380805942	32.0	32.0	32.0	32.0	32.0
5	30.58287080760946	32.0	32.0	32.0	32.0	32.0
6	34.21364800369002	36.0	36.0	36.0	32.0	36.0
7	34.07556621323527	36.0	36.0	36.0	32.0	36.0
8	34.06611520263512	36.0	36.0	36.0	32.0	36.0
9	34.24501512361084	36.0	36.0	36.0	32.0	36.0
10	33.89155663195758	36.0	36.0	36.0	32.0	36.0
11	34.2519604865343	36.0	36.0	36.0	32.0	36.0
12	34.00117971475494	36.0	36.0	36.0	32.0	36.0
13	34.13108990356912	36.0	36.0	36.0	32.0	36.0
14	34.020005303731686	36.0	36.0	36.0	32.0	36.0
15	33.96661108160901	36.0	36.0	36.0	32.0	36.0
16	33.9621939637153	36.0	36.0	36.0	32.0	36.0
17	33.88155929711598	36.0	36.0	36.0	32.0	36.0
18	33.89438263034519	36.0	36.0	36.0	32.0	36.0
19	33.88343022502311	36.0	36.0	36.0	32.0	36.0
20	33.86301949819254	36.0	36.0	36.0	32.0	36.0
21	33.83774967582768	36.0	36.0	36.0	32.0	36.0
22	33.78664974966785	36.0	36.0	36.0	32.0	36.0
23	33.74290393267052	36.0	36.0	36.0	32.0	36.0
24	33.715178309731286	36.0	36.0	36.0	27.0	36.0
25	33.22390188495156	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	8.0
4	62.0
5	146.0
6	255.0
7	65.0
8	242.0
9	258.0
10	164.0
11	48.0
12	113.0
13	78.0
14	268.0
15	342.0
16	529.0
17	716.0
18	1053.0
19	1537.0
20	2349.0
21	3401.0
22	5121.0
23	7742.0
24	10727.0
25	14629.0
26	20304.0
27	25782.0
28	34162.0
29	44781.0
30	57541.0
31	77034.0
32	110189.0
33	157336.0
34	337573.0
35	590044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.128623578552194	17.161664180711647	11.002278570535982	25.70743367020018
2	16.56591571482219	19.797944215322342	37.299697248651576	26.336442821203892
3	19.594596840070004	23.495158278980703	28.05479728845012	28.85544759249917
4	12.447627752388867	14.981105110095555	35.56692978811799	37.004337349397595
5	14.470915423990363	36.7325785414197	33.545112989410796	15.251393045179142
6	34.927997733908455	34.755979575919056	16.690016895946634	13.626005794225854
7	30.38920184358768	30.395852592794583	20.504459327343223	18.710486236274516
8	28.343272611164146	33.100888381854524	19.375239058202148	19.180599948779182
9	27.09480630933294	14.305833671507548	18.48120971014589	40.118150309013615
10	15.605025611133957	27.05577757638426	31.92954509462612	25.409651717855663
11	37.688194437975184	21.014496611069486	21.7081860005829	19.589122950372428
12	24.40573992774181	23.852456998124	28.83679484543149	22.9050082287027
13	29.71526862831211	19.50160365180128	25.12915718449315	25.653970535393462
14	23.094623982197945	19.904268713051025	25.16183684822738	31.83927045652365
15	25.251730156184816	27.563994653000634	22.54263260538466	24.641642585429892
16	25.691370338161484	25.785919552657795	24.42490323842704	24.097806870753686
17	23.736820215933857	26.16186721232387	25.76027682121409	24.34103575052819
18	24.776313486541543	24.70603815497973	26.98645935443856	23.531189004040165
19	25.02162848518083	25.95571284825189	25.282700938609707	23.739957727957577
20	25.199227046968076	24.406777179786108	25.840160552778528	24.553835220467285
21	26.02531237854903	24.834161196381892	24.42821548929102	24.71231093577806
22	25.08095644759447	25.302808731115434	24.997021335227252	24.619213486062844
23	23.668617393347297	24.99199676276541	25.600442457556223	25.73894338633107
24	24.09577215082044	25.73272603366946	25.58012468987254	24.59137712563756
25	24.655754724201152	25.140636282264158	25.191679948889785	25.01192904464491
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	204.0
1	204.0
2	456.0
3	708.0
4	708.0
5	708.0
6	2005.0
7	3302.0
8	3302.0
9	3302.0
10	3631.5
11	3961.0
12	3961.0
13	3961.0
14	4258.0
15	4555.0
16	4555.0
17	4555.0
18	7361.0
19	10167.0
20	10167.0
21	10167.0
22	16112.0
23	22057.0
24	22057.0
25	22057.0
26	32829.5
27	43602.0
28	43602.0
29	43602.0
30	54867.5
31	66133.0
32	66133.0
33	66133.0
34	81033.0
35	95933.0
36	95933.0
37	95933.0
38	108821.5
39	121710.0
40	121710.0
41	121710.0
42	140165.0
43	158620.0
44	158620.0
45	158620.0
46	185850.5
47	213081.0
48	213081.0
49	213081.0
50	206851.0
51	200621.0
52	200621.0
53	200621.0
54	183374.5
55	166128.0
56	166128.0
57	166128.0
58	152910.0
59	139692.0
60	139692.0
61	139692.0
62	122792.5
63	105893.0
64	105893.0
65	105893.0
66	87727.0
67	69561.0
68	69561.0
69	69561.0
70	52619.0
71	35677.0
72	35677.0
73	35677.0
74	28131.0
75	20585.0
76	20585.0
77	20585.0
78	16840.0
79	13095.0
80	13095.0
81	13095.0
82	9137.0
83	5179.0
84	5179.0
85	5179.0
86	3825.5
87	2472.0
88	2472.0
89	2472.0
90	1645.5
91	819.0
92	819.0
93	819.0
94	499.5
95	180.0
96	180.0
97	180.0
98	423.0
99	666.0
100	666.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009902957661200544
2	0.004386545004290174
3	0.004918247429052619
4	0.01502059349953908
5	0.03469358321574956
6	0.04585933413576091
7	0.06719389392935403
8	0.08680042084246921
9	0.09510827122938241
10	0.11557881458273657
11	0.1174397730694051
12	0.12853906118632116
13	0.11837025231273939
14	0.1231555741356014
15	0.11311969086821023
16	0.11165750920011351
17	0.12136107845202813
18	0.128937838004893
19	0.12980185444513198
20	0.11916780594988306
21	0.1287384495956071
22	0.14954130696443774
23	0.13850848165061702
24	0.1324603665689442
25	0.13066587088537096
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1504601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.99467745797644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6045106374054	41.902305211508065
2	13.884570940469859	14.993858591461255
3	4.140607085130408	6.707122321254852
4	1.6372827691716039	3.5361822011569295
5	0.809364778772779	2.185069508784133
6	0.4436102731239896	1.4371556168624793
7	0.2753269132021563	1.0406331511695492
8	0.18596002204029255	0.8032681128115029
9	0.13507175221854215	0.6563840125251507
>10	0.7134768233847557	7.160404373399489
>50	0.07442242916195559	2.817291903857422
>100	0.08211059379015835	9.43393762713117
>500	0.009246609545985314	3.368659025543387
>1k	0.004315084454793146	3.583410705576645
>5k	1.2328812727980417E-4	0.3743176369581548
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5623	0.37372034180490377	No Hit
TCCATGTACTCTGCGTTGATACCAC	4731	0.31443552144389114	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3077	0.20450604512425555	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2640	0.17546180017160698	No Hit
CATGTACTCTGCGTTGATACCACTG	2217	0.14734803446229267	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2203	0.14641755521895838	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1858	0.12348788815107793	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1693	0.11252152564035249	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1596	0.10607463374010784	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1530	0.10168808873581767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3292560619061134E-4
2	0.0	0.0	0.0	0.0	1.3292560619061134E-4
3	0.0	0.0	0.0	0.0	1.3292560619061134E-4
4	0.0	6.646280309530567E-5	0.0	0.0	1.3292560619061134E-4
5	0.0	6.646280309530567E-5	0.0	0.0	1.3292560619061134E-4
6	0.0	6.646280309530567E-5	0.0	0.0	1.99388409285917E-4
7	0.0	6.646280309530567E-5	0.0	0.0	1.99388409285917E-4
8	0.0	6.646280309530567E-5	0.0	0.0	1.99388409285917E-4
9	0.0	6.646280309530567E-5	0.0	6.646280309530567E-5	1.99388409285917E-4
10	0.0	6.646280309530567E-5	0.0	6.646280309530567E-5	2.658512123812227E-4
11	0.0	1.3292560619061134E-4	0.0	6.646280309530567E-5	2.658512123812227E-4
12	0.0	1.3292560619061134E-4	0.0	6.646280309530567E-5	7.97553637143668E-4
13	0.0	1.3292560619061134E-4	0.0	1.3292560619061134E-4	0.0010634048495248908
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	155	0.0	15.322205	7
TAAGACA	150	0.0	14.561946	4
TTCACGA	40	0.0053008785	14.241117	2
TCTAGTA	60	2.583294E-5	14.241117	2
CGACTTT	90	3.62852E-8	13.725083	13
TAAGACT	100	1.01154E-8	13.295691	4
CGCGTAA	125	1.0186341E-10	12.920974	10
CTAAGAC	125	1.03682396E-10	12.913236	3
GTATTAG	250	0.0	12.908939	1
CGCGGTC	185	0.0	12.838805	10
GAACCGC	200	0.0	12.824686	6
CGAACGT	115	5.347829E-9	12.387289	4
GTGTTAA	115	5.3914846E-9	12.380697	1
AAGACGG	300	0.0	12.347642	5
GAATAAG	70	1.09960965E-4	12.20383	1
GTATATA	140	5.2750693E-11	12.20383	1
AGAACCG	230	0.0	11.975974	5
TCGCGTA	135	3.7107384E-10	11.963467	9
CGAACGA	120	9.958967E-9	11.879059	16
ACTGCGC	80	2.8659537E-5	11.875104	8
>>END_MODULE