FastQCFastQC Report
Thu 2 Feb 2017
SRR4062589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504601
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22570.15000654658610488No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21010.1396383493032372No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16510.10973008791034965No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16020.10647341055867968No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15100.10035883267391155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT300.005745646529.3331348
TAGGACG5750.022.9563664
CCGTCGT3450.021.0433359
ACCGTCG3500.020.7427168
TGCGCGA654.933161E-420.30755410
CGTCGTA3600.019.55542410
AGGACGT6350.019.4014435
GAGCGAA5650.019.07951716
AGCGAAA5700.018.91215317
GACGGAC5950.018.8570147
ATACCGT4350.018.7125176
TACCGTC4150.018.5540927
ACGGACC5950.018.4872688
AACCGTC600.00741321618.3332087
CGGACCA5900.018.2710639
GACGTGA3650.018.082077
ATACGAA5500.017.99987840
GGATATA1601.0986696E-917.8784431
CGCGGGA3950.017.82266444
AAGACGG6550.017.8014055
Produced by FastQC (version 0.11.5)