##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1504601
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27015268499755	32.0	32.0	32.0	32.0	32.0
2	31.39917692464647	32.0	32.0	32.0	32.0	32.0
3	31.487613659701143	32.0	32.0	32.0	32.0	32.0
4	31.593158584900582	32.0	32.0	32.0	32.0	32.0
5	31.528102799346804	32.0	32.0	32.0	32.0	32.0
6	35.11237530747354	36.0	36.0	36.0	36.0	36.0
7	35.12836492864221	36.0	36.0	36.0	36.0	36.0
8	35.06694665230184	36.0	36.0	36.0	36.0	36.0
9	35.196338431251874	36.0	36.0	36.0	36.0	36.0
10	35.083747119668274	36.0	36.0	36.0	36.0	36.0
11	35.20572763144515	36.0	36.0	36.0	36.0	36.0
12	35.114037542178956	36.0	36.0	36.0	36.0	36.0
13	35.161015445290815	36.0	36.0	36.0	36.0	36.0
14	35.11891989969434	36.0	36.0	36.0	36.0	36.0
15	35.08488961525348	36.0	36.0	36.0	36.0	36.0
16	35.08825728548632	36.0	36.0	36.0	36.0	36.0
17	35.06255013787708	36.0	36.0	36.0	36.0	36.0
18	35.07550573208445	36.0	36.0	36.0	36.0	36.0
19	35.06232748748672	36.0	36.0	36.0	36.0	36.0
20	35.04802535688864	36.0	36.0	36.0	36.0	36.0
21	35.03572375666373	36.0	36.0	36.0	36.0	36.0
22	35.018756467661525	36.0	36.0	36.0	36.0	36.0
23	34.9717234004231	36.0	36.0	36.0	36.0	36.0
24	34.94663369225462	36.0	36.0	36.0	32.0	36.0
25	34.91856512125141	36.0	36.0	36.0	32.0	36.0
26	34.870969114070775	36.0	36.0	36.0	32.0	36.0
27	34.852231920622145	36.0	36.0	36.0	32.0	36.0
28	34.82094322680897	36.0	36.0	36.0	32.0	36.0
29	34.78945182144635	36.0	36.0	36.0	32.0	36.0
30	34.77898924698309	36.0	36.0	36.0	32.0	36.0
31	34.7715859553463	36.0	36.0	36.0	32.0	36.0
32	34.737235984822554	36.0	36.0	36.0	32.0	36.0
33	34.71002478397927	36.0	36.0	36.0	32.0	36.0
34	34.69075987587407	36.0	36.0	36.0	32.0	36.0
35	34.64943330490941	36.0	36.0	36.0	32.0	36.0
36	34.61337125257793	36.0	36.0	36.0	32.0	36.0
37	34.58989659052467	36.0	36.0	36.0	32.0	36.0
38	34.54161335796002	36.0	36.0	36.0	32.0	36.0
39	34.492840294536556	36.0	36.0	36.0	32.0	36.0
40	34.46514391523068	36.0	36.0	36.0	32.0	36.0
41	34.44170381383503	36.0	36.0	36.0	32.0	36.0
42	34.37875822227953	36.0	36.0	36.0	32.0	36.0
43	34.38554739761571	36.0	36.0	36.0	32.0	36.0
44	34.34595882895199	36.0	36.0	36.0	32.0	36.0
45	34.266305153326364	36.0	36.0	36.0	32.0	36.0
46	34.294758543959496	36.0	36.0	36.0	32.0	36.0
47	34.23679433949599	36.0	36.0	36.0	32.0	36.0
48	34.2172914945557	36.0	36.0	36.0	32.0	36.0
49	34.20344662804292	36.0	36.0	36.0	32.0	36.0
50	33.68048672039963	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	4.0
20	12.0
21	43.0
22	153.0
23	471.0
24	1131.0
25	2853.0
26	5779.0
27	10633.0
28	17738.0
29	26234.0
30	36741.0
31	51774.0
32	75440.0
33	118288.0
34	256763.0
35	900543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57199757508722	17.688302986471538	11.566461754445674	26.173237683995577
2	15.976242477737292	20.19170055312808	36.99584057256266	26.83621639657196
3	18.977104756638344	23.7721588310984	28.39400980465136	28.8567266076119
4	12.29418090413519	15.390689074423866	35.698040273880466	36.61708974756048
5	14.232477580434946	36.85521942362128	33.559528406534355	15.352774589409417
6	35.129218098243506	35.05285167766857	16.325731695321245	13.49219852876668
7	30.413552049416488	30.36064758782905	20.521614064611235	18.704186298143227
8	28.313486432615694	33.21784313582139	19.306314431533675	19.16235600002924
9	27.165607360356663	14.053426788896193	18.45166924653114	40.329296604216005
10	15.603671671094196	27.006894186565077	32.00423235130111	25.38520179103962
11	37.91569990980997	20.847852686526195	21.727222034280185	19.509225369383643
12	24.342201022064984	23.7884329466749	28.881477547868172	22.98788848339194
13	29.955516445888314	19.35722493870468	25.17371715159036	25.51354146381665
14	23.22961369824957	19.713465563295518	25.1462015511089	31.910719187346015
15	25.28617221442761	27.531551554199417	22.566780162980084	24.615496068392883
16	25.752408778141184	25.687208768304686	24.388658521428606	24.171723932125527
17	23.917902487104556	26.02184898188955	25.73286871403116	24.327379816974734
18	25.034743430318073	24.466486463853208	27.078341699892526	23.420428405936192
19	25.14799604679247	25.75573191829595	25.34997650539911	23.746295529512476
20	25.416904548116076	24.19305849191912	25.93810584998947	24.451931109975337
21	26.149856340651112	24.644473850542436	24.50410441040515	24.701565398401303
22	25.14154915489223	25.08186555771264	25.210936321323725	24.565648966071404
23	23.762313065058443	24.701233084385827	25.709075030523042	25.82737882003269
24	24.259056055392758	25.521849314203564	25.649856672965125	24.56923795743855
25	24.828575815116434	24.681493631866523	25.428734927067044	25.06119562595
26	23.896368538901676	25.493403234478773	25.94960391492495	24.660624311694594
27	25.17085925105726	25.151053335734858	25.26683153872688	24.411255874481007
28	24.213263184060093	24.689336242631768	25.898294630935375	25.199105942372764
29	24.01905887341561	25.29354958557119	26.08372585157128	24.603665689441918
30	24.463229786501536	25.035075744333547	26.285905698587197	24.215788770577714
31	24.65843103919245	25.015668605829717	25.160956293396058	25.164944061581775
32	24.203958391626752	25.268493108804265	25.402016880222728	25.12553161934626
33	23.94329127788696	24.8389440123993	25.864930303781534	25.352834405932207
34	24.427472798436263	25.310829914375972	26.270286939859805	23.991410347327964
35	25.36074347950055	24.95492160380061	25.837215314890795	23.847119601808053
36	24.10685623630451	25.54584238612097	25.68528134701492	24.6620200305596
37	25.266033985089738	25.242439689990903	25.330037664470513	24.16148866044885
38	24.33861203069784	25.151717963765808	25.512012819345458	24.997657186190892
39	24.878223529028627	24.88380640448863	25.337481498417187	24.900488568065555
40	25.24596221855495	25.072959542097873	25.473996095974943	24.20708214337223
41	23.934717576287667	25.313754277712164	26.28922883874197	24.462299307258203
42	25.5411899899043	25.579539027290295	25.52125114897571	23.358019833829697
43	24.606922366793587	24.409461378797435	25.66421263843371	25.31940361597527
44	24.118487226846185	25.201498603284193	25.86725650188987	24.812757667979753
45	24.38686402574503	25.370513511555554	25.483566739620674	24.759055723078742
46	23.9651070051841	24.92024458327795	26.092582746244847	25.022065665293102
47	24.656902394721257	24.58073602237404	26.29680559829483	24.465555984609875
48	24.830370310800006	25.961234905466636	24.864199877575516	24.344194906157846
49	23.975060497766517	25.36054409109126	25.801790640840995	24.862604770301232
50	23.7714849318856	26.30936706807984	24.979579303748967	24.939568696285594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	12.0
2	19.0
3	80.5
4	142.0
5	165.5
6	189.0
7	195.0
8	201.0
9	265.5
10	330.0
11	528.0
12	726.0
13	1328.5
14	1931.0
15	2810.5
16	3690.0
17	4373.5
18	5057.0
19	5508.0
20	5959.0
21	6722.5
22	7486.0
23	8077.0
24	8668.0
25	9432.5
26	10197.0
27	12598.5
28	15000.0
29	17863.5
30	20727.0
31	23257.5
32	25788.0
33	30260.5
34	34733.0
35	40268.5
36	45804.0
37	52616.0
38	59428.0
39	63487.0
40	67546.0
41	72906.5
42	78267.0
43	80732.5
44	83198.0
45	92849.0
46	102500.0
47	110213.0
48	117926.0
49	118809.5
50	119693.0
51	112148.5
52	104604.0
53	102305.5
54	100007.0
55	100231.0
56	100455.0
57	96801.5
58	93148.0
59	85104.0
60	77060.0
61	68423.5
62	59787.0
63	52030.5
64	44274.0
65	38225.5
66	32177.0
67	28183.0
68	24189.0
69	22088.0
70	19987.0
71	15598.5
72	11210.0
73	10211.0
74	9212.0
75	6671.0
76	4130.0
77	3768.0
78	3406.0
79	2895.0
80	2384.0
81	1876.5
82	1369.0
83	1167.5
84	966.0
85	783.0
86	600.0
87	451.5
88	303.0
89	191.5
90	80.0
91	51.5
92	23.0
93	14.0
94	5.0
95	6.5
96	8.0
97	7.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01442242827168133
2	0.00445300780738548
3	5.98165227857751E-4
4	1.99388409285917E-4
5	0.0
6	8.640164402389736E-4
7	1.3292560619061134E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.646280309530567E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1504601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.18515856355392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67586702710354	44.41906772506445
2	13.977644719445614	15.986276592530391
3	4.171954873442943	7.157217028734786
4	1.6343704806532193	3.7384694035098467
5	0.763558667587799	2.1832111739292124
6	0.4221092466476198	1.4483030520411857
7	0.26774015264438544	1.0717534157959518
8	0.16575983976197806	0.7583202176206404
9	0.1253414366403567	0.6450902935976199
>10	0.6493468686099328	6.7531164276507845
>50	0.06550780479275144	2.6228455676067854
>100	0.07122456040845561	8.613513267192975
>500	0.007355881737452127	2.893986403686029
>1k	0.0022184405239934987	1.7088294310393037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2257	0.15000654658610488	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2101	0.1396383493032372	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1651	0.10973008791034965	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1602	0.10647341055867968	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1510	0.10035883267391155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.646280309530567E-5
2	0.0	0.0	0.0	0.0	6.646280309530567E-5
3	0.0	0.0	0.0	0.0	6.646280309530567E-5
4	0.0	0.0	0.0	0.0	6.646280309530567E-5
5	0.0	0.0	0.0	0.0	6.646280309530567E-5
6	0.0	0.0	0.0	0.0	1.99388409285917E-4
7	0.0	0.0	0.0	0.0	1.99388409285917E-4
8	0.0	0.0	0.0	0.0	1.99388409285917E-4
9	0.0	0.0	0.0	0.0	1.99388409285917E-4
10	0.0	0.0	0.0	0.0	1.99388409285917E-4
11	0.0	0.0	0.0	0.0	1.99388409285917E-4
12	0.0	0.0	0.0	0.0	6.646280309530566E-4
13	0.0	0.0	0.0	0.0	7.310908340483623E-4
14	0.0	0.0	0.0	0.0	8.640164402389736E-4
15	0.0	0.0	0.0	0.0	0.0010634048495248908
16	0.0	0.0	0.0	6.646280309530567E-5	0.0010634048495248908
17	0.0	0.0	0.0	1.3292560619061134E-4	0.0010634048495248908
18	0.0	0.0	0.0	5.317024247624454E-4	0.0010634048495248908
19	0.0	0.0	0.0	6.646280309530566E-4	0.0011298676526201963
20	0.0	0.0	0.0	0.0011298676526201963	0.001395718865001419
21	0.0	0.0	0.0	0.001395718865001419	0.0014621816680967245
22	0.0	0.0	0.0	0.0018609584866685585	0.001595107274287336
23	0.0	0.0	0.0	0.002791437730002838	0.0016615700773826415
24	0.0	0.0	0.0	0.005383487050719759	0.0017280328804779473
25	0.0	6.646280309530567E-5	0.0	0.006646280309530566	0.0017280328804779473
26	0.0	6.646280309530567E-5	0.0	0.008640164402389737	0.0019274212897638643
27	0.0	1.3292560619061134E-4	0.0	0.011298676526201963	0.0019274212897638643
28	0.0	1.3292560619061134E-4	0.0	0.016482775167635806	0.00199388409285917
29	0.0	1.3292560619061134E-4	0.0	0.02791437730002838	0.00199388409285917
30	0.0	1.3292560619061134E-4	0.0	0.0469227389852858	0.00199388409285917
31	0.0	1.3292560619061134E-4	0.0	0.0879302884950894	0.0020603468959544758
32	0.0	1.3292560619061134E-4	0.0	0.13285914338751603	0.0020603468959544758
33	0.0	1.3292560619061134E-4	0.0	0.17964895676661122	0.0020603468959544758
34	0.0	1.3292560619061134E-4	0.0	0.2346801577295243	0.0020603468959544758
35	0.0	1.99388409285917E-4	0.0	0.29250279642244026	0.0020603468959544758
36	0.0	2.658512123812227E-4	0.0	0.37598007711014414	0.0020603468959544758
37	0.0	2.658512123812227E-4	0.0	0.5027911054159874	0.0020603468959544758
38	0.0	2.658512123812227E-4	0.0	0.6780535171783084	0.0020603468959544758
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCGT	30	0.0057456465	29.333134	8
TAGGACG	575	0.0	22.956366	4
CCGTCGT	345	0.0	21.043335	9
ACCGTCG	350	0.0	20.742716	8
TGCGCGA	65	4.933161E-4	20.307554	10
CGTCGTA	360	0.0	19.555424	10
AGGACGT	635	0.0	19.401443	5
GAGCGAA	565	0.0	19.079517	16
AGCGAAA	570	0.0	18.912153	17
GACGGAC	595	0.0	18.857014	7
ATACCGT	435	0.0	18.712517	6
TACCGTC	415	0.0	18.554092	7
ACGGACC	595	0.0	18.487268	8
AACCGTC	60	0.007413216	18.333208	7
CGGACCA	590	0.0	18.271063	9
GACGTGA	365	0.0	18.08207	7
ATACGAA	550	0.0	17.999878	40
GGATATA	160	1.0986696E-9	17.878443	1
CGCGGGA	395	0.0	17.822664	44
AAGACGG	655	0.0	17.801405	5
>>END_MODULE