FastQCFastQC Report
Thu 2 Feb 2017
SRR4062588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792984
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG75580.9531087638590439No Hit
TCCATGTACTCTGCGTTGATACCAC61980.7816046729820525No Hit
GTATCAACGCAGAGTACTTTTTTTT44590.5623064273680175No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34950.4407402923640326No Hit
GAGTACATGGAAGCAGTGGTATCAA33760.42573368441229587No Hit
TATCAACGCAGAGTACTTTTTTTTT32710.4124925597489987No Hit
CATGTACTCTGCGTTGATACCACTG29070.36658999424956873No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25950.3272449381072001No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20130.2538512756877818No Hit
GCTTCCATGTACTCTGCGTTGATAC19090.240736256973659No Hit
CCCATGTACTCTGCGTTGATACCAC19050.24023183317696198No Hit
GCGTTGATACCACTGCTTCCATGTA18970.2392229855835679No Hit
GTACATGGGAAGCAGTGGTATCAAC17910.2258557549710965No Hit
CATGGAAGCAGTGGTATCAACGCAG16780.21160578271440536No Hit
GGTATCAACGCAGAGTACTTTTTTT16280.20530048525569242No Hit
GTACATGGGGTGGTATCAACGCAAA15960.2012650948821162No Hit
GTATCAACGCAGAGTACATGGAAGC13710.17289125631790805No Hit
ACTCTGCGTTGATACCACTGCTTCC12330.15548863533186041No Hit
GTATCAACGCAGAGTACATGGGAAG12130.15296651634837524No Hit
ACGCAGAGTACATGGAAGCAGTGGT11430.14413909990617718No Hit
CAGTGGTATCAACGCAGAGTACATG11350.14313025231278312No Hit
GTACATGGGTGGTATCAACGCAAAA10820.13644663700654744No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8750.11034270552747598No Hit
TATCAACGCAGAGTACATGGAAGCA8260.1041635140179373No Hit
GTACATGGGAGTGGTATCAACGCAA7980.1006325474410581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCC504.555448E-617.1004544
CCGTCGT801.2842793E-714.2512799
TTCCGGT400.005275146614.2503811
TAGAAAT602.56448E-514.2503794
TAGTACT551.9577997E-413.8185494
AAGACGG1800.013.7225885
CGGTGTT500.001497401313.30119414
CGGACCA1800.013.1956289
CAAGACG1900.013.0003454
GCATCGC1251.0186341E-1012.92116114
GACGGAC1850.012.838997
CGGCTTA604.0828175E-412.66860319
CGAACGA604.0849939E-412.66780316
CCGATAA604.0849939E-412.6678039
ACGAACG604.0849939E-412.66780315
TAACGAA604.087171E-412.66700313
TTCGTCA853.929692E-612.29599818
GAGTGTT853.9324677E-612.2952217
TTTTCGT853.9324677E-612.29522116
AGAGCGA2100.012.21538315
Produced by FastQC (version 0.11.5)