##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062588_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792984
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.013050704680044	32.0	32.0	32.0	32.0	32.0
2	30.845290699434038	32.0	32.0	32.0	32.0	32.0
3	30.86721295763849	32.0	32.0	32.0	32.0	32.0
4	30.860399453204604	32.0	32.0	32.0	32.0	32.0
5	30.777019460670076	32.0	32.0	32.0	32.0	32.0
6	34.373622923035015	36.0	36.0	36.0	32.0	36.0
7	34.188079456836455	36.0	36.0	36.0	32.0	36.0
8	34.16710299325081	36.0	36.0	36.0	32.0	36.0
9	34.24763046921502	36.0	36.0	36.0	32.0	36.0
10	34.069261170464976	36.0	36.0	36.0	32.0	36.0
11	34.34622640557691	36.0	36.0	36.0	32.0	36.0
12	34.156917415736004	36.0	36.0	36.0	32.0	36.0
13	34.257002663357646	36.0	36.0	36.0	32.0	36.0
14	34.184180260887985	36.0	36.0	36.0	32.0	36.0
15	34.178876244665716	36.0	36.0	36.0	32.0	36.0
16	34.15188074412598	36.0	36.0	36.0	32.0	36.0
17	34.07786664043663	36.0	36.0	36.0	32.0	36.0
18	34.10367043975667	36.0	36.0	36.0	32.0	36.0
19	34.085962642373616	36.0	36.0	36.0	32.0	36.0
20	34.017735540691866	36.0	36.0	36.0	32.0	36.0
21	33.94261800994724	36.0	36.0	36.0	32.0	36.0
22	33.946886696326786	36.0	36.0	36.0	32.0	36.0
23	33.91076995248328	36.0	36.0	36.0	32.0	36.0
24	33.90225906197351	36.0	36.0	36.0	32.0	36.0
25	33.4124005024061	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	24.0
5	89.0
6	146.0
7	29.0
8	125.0
9	122.0
10	95.0
11	29.0
12	62.0
13	47.0
14	284.0
15	310.0
16	359.0
17	539.0
18	721.0
19	980.0
20	1525.0
21	2062.0
22	2919.0
23	3994.0
24	5243.0
25	6858.0
26	9020.0
27	11492.0
28	15659.0
29	20505.0
30	26348.0
31	36879.0
32	52638.0
33	77031.0
34	174063.0
35	342782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.62678819385327	17.292904986574754	11.550758031501724	24.529548788070258
2	15.546153312609166	20.998669533580088	37.507929201530985	25.94724795227976
3	19.43201895708299	25.77315817284592	28.14392855025985	26.650894319811236
4	12.24304982733352	15.67708215090218	35.65972721470932	36.420140807054985
5	13.716407234578835	37.641432433489385	33.293973833501745	15.348186498430035
6	33.088690802879185	34.850394594901495	17.86319448387239	14.197720118346929
7	28.899814246486837	31.536327935713132	21.43471874495235	18.129139072847682
8	28.718851013718616	31.955202762656178	20.52138067175661	18.804565551868592
9	27.445280351417534	14.960869455454292	19.175102875463885	38.418747317664284
10	15.854616250940623	27.343604013999506	31.90266000696945	24.899119728090422
11	36.06486175275213	22.197966473195855	21.808710100590513	19.928461673461502
12	24.021042024701398	24.87678975274611	29.098579562314775	22.003588660237718
13	29.748808434077205	20.219690035036773	25.810675168081815	24.220826362804203
14	23.89561097930679	19.986010083346066	25.987154024184374	30.131224913162768
15	25.115898554676846	26.816880283610583	24.94419699219263	23.123024169519944
16	24.82274696750866	26.331417721263296	25.469013543949664	23.376821767278383
17	22.88221836143069	26.215770547772244	27.07775679033545	23.82425430046161
18	24.215283442474323	24.484747620406544	28.82976278565855	22.470206151460587
19	23.883635151397296	27.182819541798885	26.788589294666277	22.14495601213754
20	24.47213219075084	23.967464934022047	28.303032675216254	23.257370200010858
21	25.478801654197053	25.39697572371121	25.137228904252297	23.986993717839443
22	24.040981442913562	25.832151386824254	26.14133091176385	23.985536258498332
23	22.65563435776202	25.50299549413025	26.479561940554845	25.361808207552887
24	22.865159529801584	27.18907567800323	26.40250732080599	23.543257471389193
25	24.166790834636046	25.399589361793474	26.01137967871222	24.42224012485826
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	290.0
1	290.0
2	616.0
3	942.0
4	942.0
5	942.0
6	2782.5
7	4623.0
8	4623.0
9	4623.0
10	4559.5
11	4496.0
12	4496.0
13	4496.0
14	3826.0
15	3156.0
16	3156.0
17	3156.0
18	5097.0
19	7038.0
20	7038.0
21	7038.0
22	10807.5
23	14577.0
24	14577.0
25	14577.0
26	21022.0
27	27467.0
28	27467.0
29	27467.0
30	32852.0
31	38237.0
32	38237.0
33	38237.0
34	44600.0
35	50963.0
36	50963.0
37	50963.0
38	56418.0
39	61873.0
40	61873.0
41	61873.0
42	72543.5
43	83214.0
44	83214.0
45	83214.0
46	107455.5
47	131697.0
48	131697.0
49	131697.0
50	119566.5
51	107436.0
52	107436.0
53	107436.0
54	93044.0
55	78652.0
56	78652.0
57	78652.0
58	71078.5
59	63505.0
60	63505.0
61	63505.0
62	55939.5
63	48374.0
64	48374.0
65	48374.0
66	39806.0
67	31238.0
68	31238.0
69	31238.0
70	23516.5
71	15795.0
72	15795.0
73	15795.0
74	12322.0
75	8849.0
76	8849.0
77	8849.0
78	7544.5
79	6240.0
80	6240.0
81	6240.0
82	4304.0
83	2368.0
84	2368.0
85	2368.0
86	1729.5
87	1091.0
88	1091.0
89	1091.0
90	740.0
91	389.0
92	389.0
93	389.0
94	240.5
95	92.0
96	92.0
97	92.0
98	237.0
99	382.0
100	382.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00945794618806937
2	0.0036570725260534893
3	0.004413708221099038
4	0.015889349595956538
5	0.03669683120970915
6	0.05031627372052905
7	0.06759278875740242
8	0.08865248226950355
9	0.0963449451691333
10	0.12055728741059088
11	0.12118781715646217
12	0.13178071688709986
13	0.12093560525811364
14	0.12421435993664437
15	0.11500862564692352
16	0.11299093046013538
17	0.12307940639407605
18	0.1314023990395771
19	0.133798412073888
20	0.1232055123432503
21	0.13354620017553948
22	0.15271430445002673
23	0.1422475106685633
24	0.13405062397223652
25	0.13190682283627414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	792984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.388200320942424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17811331269358	40.68820747946976
2	13.158491103156877	13.523823534607285
3	3.611004701492651	5.566890988805077
4	1.3502673995988541	2.775512464696957
5	0.6913329485198213	1.7763178023502177
6	0.40109698675947936	1.236699138223351
7	0.25593361979600526	0.9206377686058723
8	0.1777753329310387	0.730844353662596
9	0.1357648268456893	0.627903910663591
>10	0.8131476829753398	7.95851486740919
>50	0.10726281607618482	3.892182575703196
>100	0.10603243327657541	10.507870834404256
>500	0.008364507497296983	2.7840794484022187
>1k	0.004920298527821754	5.271444953229829
>5k	4.920298527821754E-4	1.7390698797666388
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7558	0.9531087638590439	No Hit
TCCATGTACTCTGCGTTGATACCAC	6198	0.7816046729820525	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4459	0.5623064273680175	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3495	0.4407402923640326	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3376	0.42573368441229587	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3271	0.4124925597489987	No Hit
CATGTACTCTGCGTTGATACCACTG	2907	0.36658999424956873	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2595	0.3272449381072001	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2013	0.2538512756877818	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1909	0.240736256973659	No Hit
CCCATGTACTCTGCGTTGATACCAC	1905	0.24023183317696198	No Hit
GCGTTGATACCACTGCTTCCATGTA	1897	0.2392229855835679	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1791	0.2258557549710965	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1678	0.21160578271440536	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1628	0.20530048525569242	No Hit
GTACATGGGGTGGTATCAACGCAAA	1596	0.2012650948821162	No Hit
GTATCAACGCAGAGTACATGGAAGC	1371	0.17289125631790805	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1233	0.15548863533186041	No Hit
GTATCAACGCAGAGTACATGGGAAG	1213	0.15296651634837524	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1143	0.14413909990617718	No Hit
CAGTGGTATCAACGCAGAGTACATG	1135	0.14313025231278312	No Hit
GTACATGGGTGGTATCAACGCAAAA	1082	0.13644663700654744	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	875	0.11034270552747598	No Hit
TATCAACGCAGAGTACATGGAAGCA	826	0.1041635140179373	No Hit
GTACATGGGAGTGGTATCAACGCAA	798	0.1006325474410581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2610594917425823E-4	0.0
2	0.0	0.0	0.0	1.2610594917425823E-4	0.0
3	0.0	0.0	0.0	1.2610594917425823E-4	0.0
4	0.0	0.0	0.0	1.2610594917425823E-4	0.0
5	0.0	0.0	0.0	1.2610594917425823E-4	0.0
6	0.0	0.0	0.0	1.2610594917425823E-4	0.0
7	0.0	0.0	0.0	1.2610594917425823E-4	0.0
8	0.0	0.0	0.0	1.2610594917425823E-4	0.0
9	0.0	0.0	0.0	2.5221189834851647E-4	0.0
10	0.0	0.0	0.0	2.5221189834851647E-4	0.0
11	0.0	0.0	0.0	2.5221189834851647E-4	0.0
12	0.0	0.0	0.0	2.5221189834851647E-4	1.2610594917425823E-4
13	0.0	0.0	0.0	2.5221189834851647E-4	2.5221189834851647E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCC	50	4.555448E-6	17.100454	4
CCGTCGT	80	1.2842793E-7	14.251279	9
TTCCGGT	40	0.0052751466	14.25038	11
TAGAAAT	60	2.56448E-5	14.250379	4
TAGTACT	55	1.9577997E-4	13.818549	4
AAGACGG	180	0.0	13.722588	5
CGGTGTT	50	0.0014974013	13.301194	14
CGGACCA	180	0.0	13.195628	9
CAAGACG	190	0.0	13.000345	4
GCATCGC	125	1.0186341E-10	12.921161	14
GACGGAC	185	0.0	12.83899	7
CGGCTTA	60	4.0828175E-4	12.668603	19
CGAACGA	60	4.0849939E-4	12.667803	16
CCGATAA	60	4.0849939E-4	12.667803	9
ACGAACG	60	4.0849939E-4	12.667803	15
TAACGAA	60	4.087171E-4	12.667003	13
TTCGTCA	85	3.929692E-6	12.295998	18
GAGTGTT	85	3.9324677E-6	12.295221	7
TTTTCGT	85	3.9324677E-6	12.295221	16
AGAGCGA	210	0.0	12.215383	15
>>END_MODULE