FastQCFastQC Report
Thu 2 Feb 2017
SRR4062588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792984
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30990.39080233649102625No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28940.36495061691030334No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12560.15838907216286835No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12550.1582629662136941No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC12160.15334483419589803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.13152850498875135No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG8220.10365909022124029No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8150.10277634857702048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA500.002579705321.99993732
CTATTCG500.002579705321.99993738
GTATAGG755.4670563E-520.534571
ATCGTTT2150.020.4650629
TAGGACG2100.019.9047054
TTAGGAC1900.019.6841533
TATACTG1051.7911516E-618.8570885
GGACGTG2350.018.723356
TATACAG1658.185452E-1118.6666135
ACGAGAC1658.185452E-1118.66661319
TACTATA851.4293875E-418.1187382
CTATACT851.430078E-418.1175964
AGGACGT2350.017.7871845
TGTAGGA3000.017.601062
GTATAGA1002.4799841E-517.6010591
TAGCGTA1254.847425E-717.5999497
TGGTCGG2500.017.59994937
ATAACGC2800.017.2856643
CGGCATC2550.017.25485225
ATGGTCG2550.017.25485236
Produced by FastQC (version 0.11.5)