##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792984
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081431655619784	32.0	32.0	32.0	32.0	32.0
2	31.40112663054992	32.0	32.0	32.0	32.0	32.0
3	31.500498118499237	32.0	32.0	32.0	32.0	32.0
4	31.611861021155534	32.0	32.0	32.0	32.0	32.0
5	31.543446021609515	32.0	32.0	32.0	32.0	32.0
6	35.134681153718105	36.0	36.0	36.0	36.0	36.0
7	35.15042169829404	36.0	36.0	36.0	36.0	36.0
8	35.11039945320461	36.0	36.0	36.0	36.0	36.0
9	35.181441239671926	36.0	36.0	36.0	36.0	36.0
10	35.0899967716877	36.0	36.0	36.0	36.0	36.0
11	35.20461320783269	36.0	36.0	36.0	36.0	36.0
12	35.11228599820425	36.0	36.0	36.0	36.0	36.0
13	35.13807088163191	36.0	36.0	36.0	36.0	36.0
14	35.10686722556823	36.0	36.0	36.0	36.0	36.0
15	35.084724281952724	36.0	36.0	36.0	36.0	36.0
16	35.08158802699676	36.0	36.0	36.0	36.0	36.0
17	35.05967837938723	36.0	36.0	36.0	36.0	36.0
18	35.06587522572965	36.0	36.0	36.0	36.0	36.0
19	35.03181274779819	36.0	36.0	36.0	36.0	36.0
20	34.998633011510954	36.0	36.0	36.0	36.0	36.0
21	34.968998113455	36.0	36.0	36.0	36.0	36.0
22	34.94881737840864	36.0	36.0	36.0	36.0	36.0
23	34.91688230783976	36.0	36.0	36.0	32.0	36.0
24	34.88791324919544	36.0	36.0	36.0	32.0	36.0
25	34.862119538351344	36.0	36.0	36.0	32.0	36.0
26	34.816513321832474	36.0	36.0	36.0	32.0	36.0
27	34.78720377712539	36.0	36.0	36.0	32.0	36.0
28	34.76228776368754	36.0	36.0	36.0	32.0	36.0
29	34.71486436044107	36.0	36.0	36.0	32.0	36.0
30	34.69289418197593	36.0	36.0	36.0	32.0	36.0
31	34.67046119467732	36.0	36.0	36.0	32.0	36.0
32	34.61258613036328	36.0	36.0	36.0	32.0	36.0
33	34.56342120395872	36.0	36.0	36.0	32.0	36.0
34	34.51218940104718	36.0	36.0	36.0	32.0	36.0
35	34.43098473613591	36.0	36.0	36.0	32.0	36.0
36	34.40298291012177	36.0	36.0	36.0	32.0	36.0
37	34.34197411297075	36.0	36.0	36.0	32.0	36.0
38	34.267764545060174	36.0	36.0	36.0	32.0	36.0
39	34.171328803607636	36.0	36.0	36.0	32.0	36.0
40	34.10669824359634	36.0	36.0	36.0	32.0	36.0
41	34.07553746355538	36.0	36.0	36.0	32.0	36.0
42	33.93427357929038	36.0	36.0	36.0	32.0	36.0
43	33.954595552999805	36.0	36.0	36.0	32.0	36.0
44	33.91601595996893	36.0	36.0	36.0	32.0	36.0
45	33.747457704064644	36.0	36.0	36.0	27.0	36.0
46	33.78688851225245	36.0	36.0	36.0	32.0	36.0
47	33.67051415917597	36.0	36.0	36.0	27.0	36.0
48	33.64513912008313	36.0	36.0	36.0	27.0	36.0
49	33.63530537816652	36.0	36.0	36.0	21.0	36.0
50	33.06595215035865	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	19.0
21	22.0
22	99.0
23	281.0
24	799.0
25	1916.0
26	3967.0
27	7551.0
28	12643.0
29	18137.0
30	23852.0
31	31561.0
32	44711.0
33	63468.0
34	128419.0
35	455539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63178287240765	18.163208935740315	12.144625602722293	25.06038258912975
2	14.963345780508504	21.478754677149027	37.05727087802399	26.500628664318477
3	18.757550612750354	25.93387996196635	28.583372636453287	26.725196788830015
4	12.095704326947327	16.11507924497846	35.780040959212286	36.00917546886192
5	13.445794618806936	37.723964165733484	33.38465845464726	15.445582760812323
6	33.34451479799591	35.05843155997826	17.45405616037751	14.142997481648317
7	28.7226476196241	31.422575991444972	21.56172129576385	18.293055093167077
8	28.640678752660836	32.04553938036581	20.490072939681003	18.823708927292355
9	27.436366938046667	14.530179675756383	19.263314266113817	38.77013912008313
10	15.73007778214945	27.210132865228047	32.195479353934005	24.864309998688498
11	36.30464170777721	21.855800369238217	21.895019319431412	19.94453860355316
12	23.950420185022647	24.88070377208115	29.1905006910606	21.9783753518356
13	30.013215903473462	19.896366130968595	25.853863381858904	24.23655458369904
14	23.81712619674546	19.792707040747352	26.131422575991447	30.258744186515745
15	25.21059693512101	26.646439272419116	24.941234627684796	23.201729164775077
16	24.93871250870131	26.06143377419973	25.619936846140657	23.379916870958304
17	22.857207711631002	26.192710067290136	27.2652411650172	23.68484105606166
18	24.460644855381698	24.039450985139673	28.921264489573563	22.57863966990507
19	23.993548419640245	26.879483066493144	27.0271531329762	22.09981538089041
20	24.41537281962814	23.612456241235634	28.732483883659693	23.23968705547653
21	25.42346377732716	25.092940084541425	25.546669289670408	23.936926848461006
22	23.94890691363256	25.542633899296835	26.44391311804526	24.064546069025354
23	22.7347588349828	24.973013326876707	26.917062639347073	25.37516519879342
24	22.875240862362922	26.73887493316385	26.801297378005106	23.584586826468126
25	24.36783087678944	24.737699625717543	26.315915579633387	24.57855391785963
26	22.678641687600255	25.52144809983556	27.607240499177788	24.1926697133864
27	24.632527264106212	25.963575557640507	26.758169143387505	22.645728034865773
28	23.38155624829757	24.474894827638387	27.593873268565318	24.549675655498724
29	22.653798815612923	26.04428336511203	28.247606509084672	23.05431131019037
30	24.089262835063508	25.294205179423546	27.733220342402877	22.883311643110073
31	22.823915741049	25.678828324405035	26.811385853939047	24.68587008060692
32	22.774482208972692	26.030159498804515	26.96725280711843	24.228105485104365
33	22.662626232055125	25.47214067370842	27.284283163342515	24.58094993089394
34	22.785831744398376	26.100400512494577	28.125031526487295	22.988736216619753
35	24.652199792177395	25.611109429698452	27.12185870080607	22.614832077318077
36	23.25481976937744	26.352612410843097	26.874691040424526	23.517876779354943
37	24.364552122110915	26.076314276202297	26.81680840975354	22.742325191933254
38	23.26125117278533	26.04125682233185	26.440382151468377	24.257109853414445
39	23.23224680447525	25.82864219202405	26.957290437133665	23.98182056636704
40	23.997205492166298	25.938606579704004	26.70394358524258	23.36024434288712
41	22.416593525216147	26.18526981628885	27.529559234486445	23.868577424008556
42	24.6688457774684	26.43949940982416	26.807224357616295	22.08443045509115
43	23.532126751611635	24.828748121021356	26.50507450339477	25.134050623972236
44	22.916477507742904	25.637213361177523	27.680003631851335	23.76630549922823
45	23.435655700493328	26.012000242123424	26.661950304167547	23.8903937532157
46	22.549749047895283	25.71124114101238	27.536507856440263	24.20250195465207
47	23.414499428108797	25.318449449735997	27.734138058445133	23.53291306371007
48	23.385717744620322	27.81216266658596	25.540868416008394	23.26125117278533
49	22.216085066029077	26.300026230037428	27.652134217083823	23.831754486849672
50	22.10677908605859	27.973361345703502	25.415803365267603	24.504056202970304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	38.5
2	44.0
3	182.0
4	320.0
5	380.5
6	441.0
7	384.5
8	328.0
9	463.5
10	599.0
11	937.5
12	1276.0
13	2073.5
14	2871.0
15	3896.0
16	4921.0
17	5636.0
18	6351.0
19	6694.5
20	7038.0
21	7625.0
22	8212.0
23	8278.5
24	8345.0
25	8123.5
26	7902.0
27	8788.0
28	9674.0
29	11070.5
30	12467.0
31	13911.5
32	15356.0
33	17673.5
34	19991.0
35	21970.5
36	23950.0
37	27346.0
38	30742.0
39	32802.0
40	34862.0
41	37992.0
42	41122.0
43	43733.5
44	46345.0
45	52516.0
46	58687.0
47	64339.5
48	69992.0
49	67492.0
50	64992.0
51	57900.0
52	50808.0
53	48379.0
54	45950.0
55	46098.0
56	46246.0
57	44575.5
58	42905.0
59	39036.0
60	35167.0
61	31062.5
62	26958.0
63	23425.5
64	19893.0
65	17070.0
66	14247.0
67	12226.5
68	10206.0
69	9471.0
70	8736.0
71	6921.0
72	5106.0
73	4627.5
74	4149.0
75	2930.5
76	1712.0
77	1554.5
78	1397.0
79	1250.5
80	1104.0
81	877.0
82	650.0
83	535.5
84	421.0
85	354.0
86	287.0
87	208.5
88	130.0
89	78.5
90	27.0
91	19.5
92	12.0
93	9.5
94	7.0
95	4.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015511031748433764
2	0.004161496322750522
3	2.5221189834851647E-4
4	0.0
5	0.0
6	6.305297458712913E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.044237966970329E-4
47	1.2610594917425823E-4
48	0.0
49	0.0
50	1.2610594917425823E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	792984.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.04385275278665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7855354555712	46.89103724538201
2	12.304436066689968	14.283937505220598
3	3.2737389936642747	5.7006127229791534
4	1.2438211781933013	2.8878469327139835
5	0.5968850667267447	1.7322754461712202
6	0.36994127256440973	1.2883690051144268
7	0.22482523435922988	0.9134805958780523
8	0.16945081031693865	0.7868462306301415
9	0.12269267247157979	0.6409399873307633
>10	0.7228488773828907	7.978060053489785
>50	0.09247546388672076	3.8083446639345535
>100	0.08613458974399324	9.496924469020117
>500	0.005902624168914474	2.2255759899622056
>1k	0.001311694259758772	1.3657491521730554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3099	0.39080233649102625	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2894	0.36495061691030334	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1256	0.15838907216286835	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1255	0.1582629662136941	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1216	0.15334483419589803	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1043	0.13152850498875135	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	822	0.10365909022124029	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	815	0.10277634857702048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.2610594917425823E-4	0.0	0.0	0.0
6	0.0	1.2610594917425823E-4	0.0	0.0	0.0
7	0.0	1.2610594917425823E-4	0.0	0.0	0.0
8	0.0	1.2610594917425823E-4	0.0	0.0	0.0
9	0.0	1.2610594917425823E-4	0.0	0.0	0.0
10	0.0	1.2610594917425823E-4	0.0	0.0	0.0
11	0.0	1.2610594917425823E-4	0.0	0.0	0.0
12	0.0	1.2610594917425823E-4	0.0	0.0	1.2610594917425823E-4
13	0.0	1.2610594917425823E-4	0.0	0.0	3.7831784752277476E-4
14	0.0	1.2610594917425823E-4	0.0	0.0	3.7831784752277476E-4
15	0.0	1.2610594917425823E-4	0.0	0.0	3.7831784752277476E-4
16	0.0	1.2610594917425823E-4	0.0	3.7831784752277476E-4	5.044237966970329E-4
17	0.0	1.2610594917425823E-4	0.0	3.7831784752277476E-4	5.044237966970329E-4
18	0.0	1.2610594917425823E-4	0.0	8.827416442198077E-4	5.044237966970329E-4
19	0.0	1.2610594917425823E-4	0.0	0.001513271390091099	5.044237966970329E-4
20	0.0	1.2610594917425823E-4	0.0	0.0018915892376138736	5.044237966970329E-4
21	0.0	1.2610594917425823E-4	0.0	0.0032787546785307143	7.566356950455495E-4
22	0.0	1.2610594917425823E-4	0.0	0.00504423796697033	8.827416442198077E-4
23	0.0	1.2610594917425823E-4	0.0	0.00933184023889511	0.0010088475933940659
24	0.0	1.2610594917425823E-4	0.0	0.01664598529100209	0.0010088475933940659
25	0.0	1.2610594917425823E-4	0.0	0.019798634020358544	0.0010088475933940659
26	0.0	1.2610594917425823E-4	0.0	0.025221189834851648	0.0011349535425683242
27	0.0	1.2610594917425823E-4	0.0	0.03303975868365566	0.0012610594917425826
28	0.0	1.2610594917425823E-4	0.0	0.05359502839905975	0.0012610594917425826
29	0.0	1.2610594917425823E-4	0.0	0.08007727772565398	0.0012610594917425826
30	0.0	1.2610594917425823E-4	0.0	0.12938470385278897	0.0012610594917425826
31	0.0	1.2610594917425823E-4	0.0	0.2165239147322014	0.0012610594917425826
32	0.0	1.2610594917425823E-4	0.0	0.33228917607417047	0.0012610594917425826
33	0.0	1.2610594917425823E-4	0.0	0.4351916306003652	0.0012610594917425826
34	0.0	1.2610594917425823E-4	0.0	0.5615497916729719	0.0013871654409168407
35	0.0	1.2610594917425823E-4	0.0	0.7043017261382323	0.0013871654409168407
36	0.0	1.2610594917425823E-4	0.0	0.8982526759682414	0.0013871654409168407
37	0.0	1.2610594917425823E-4	0.0	1.19346670298518	0.0013871654409168407
38	0.0	1.2610594917425823E-4	0.0	1.5340788717048515	0.0013871654409168407
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	50	0.0025797053	21.999937	32
CTATTCG	50	0.0025797053	21.999937	38
GTATAGG	75	5.4670563E-5	20.53457	1
ATCGTTT	215	0.0	20.46506	29
TAGGACG	210	0.0	19.904705	4
TTAGGAC	190	0.0	19.684153	3
TATACTG	105	1.7911516E-6	18.857088	5
GGACGTG	235	0.0	18.72335	6
TATACAG	165	8.185452E-11	18.666613	5
ACGAGAC	165	8.185452E-11	18.666613	19
TACTATA	85	1.4293875E-4	18.118738	2
CTATACT	85	1.430078E-4	18.117596	4
AGGACGT	235	0.0	17.787184	5
TGTAGGA	300	0.0	17.60106	2
GTATAGA	100	2.4799841E-5	17.601059	1
TAGCGTA	125	4.847425E-7	17.599949	7
TGGTCGG	250	0.0	17.599949	37
ATAACGC	280	0.0	17.285664	3
CGGCATC	255	0.0	17.254852	25
ATGGTCG	255	0.0	17.254852	36
>>END_MODULE