FastQCFastQC Report
Thu 2 Feb 2017
SRR4062587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16565
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT970.5855719891337157No Hit
TATCAACGCAGAGTACTTTTTTTTT770.46483549652882583No Hit
GGTATCAACGCAGAGTACTTTTTTT490.2958044068819801No Hit
GTACATGGAAGCAGTGGTATCAACG450.27165710836100215No Hit
GTACTTTTTTTTTTTTTTTTTTTTT390.23543616057953515No Hit
TCCATGTACTCTGCGTTGATACCAC330.1992152127980682No Hit
GAGTACTTTTTTTTTTTTTTTTTTT320.19317838816782373No Hit
ACGCAGAGTACTTTTTTTTTTTTTT320.19317838816782373No Hit
GGTTAATTCCGATAACGAACGAGAC260.15695744038635678No Hit
GAGTACATGGAAGCAGTGGTATCAA190.11469966797464533No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG190.11469966797464533No Hit
GCTTTGAACACTCTAATTTTTTCAA190.11469966797464533No Hit
GCGCAAGACGGACCAGAGCGAAAGC180.10866284334440084No Hit
GTGCATGGCCGTTCTTAGTTGGTGG180.10866284334440084No Hit
GATTAAGAGGGACGGCCGGGGGCAT180.10866284334440084No Hit
GAATAACGCCGCCGCATCGCCAGTC170.10262601871415634No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)