##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062587_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16565
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.487594325384848	32.0	32.0	32.0	14.0	32.0
2	28.41943857530939	32.0	32.0	32.0	14.0	32.0
3	28.57331723513432	32.0	32.0	32.0	14.0	32.0
4	28.177120434651375	32.0	32.0	32.0	14.0	32.0
5	28.338424388771507	32.0	32.0	32.0	14.0	32.0
6	31.729791729550257	36.0	32.0	36.0	14.0	36.0
7	31.60374283127075	36.0	32.0	36.0	14.0	36.0
8	31.382070630848172	36.0	32.0	36.0	14.0	36.0
9	31.6889224268035	36.0	32.0	36.0	14.0	36.0
10	30.891940839118625	36.0	32.0	36.0	14.0	36.0
11	32.14379716269242	36.0	32.0	36.0	14.0	36.0
12	31.319951705402957	36.0	32.0	36.0	14.0	36.0
13	31.63923936009659	36.0	32.0	36.0	14.0	36.0
14	31.22143072743737	36.0	32.0	36.0	14.0	36.0
15	31.17265318442499	36.0	32.0	36.0	14.0	36.0
16	31.229278599456684	36.0	32.0	36.0	14.0	36.0
17	31.07008753395714	36.0	32.0	36.0	14.0	36.0
18	31.210141865378812	36.0	32.0	36.0	14.0	36.0
19	31.19824932085723	36.0	32.0	36.0	14.0	36.0
20	31.11216420162994	36.0	32.0	36.0	14.0	36.0
21	31.010021128886205	36.0	32.0	36.0	14.0	36.0
22	31.053124056746153	36.0	32.0	36.0	14.0	36.0
23	31.046966495623302	36.0	32.0	36.0	14.0	36.0
24	30.882221551463928	36.0	32.0	36.0	14.0	36.0
25	30.274252942952007	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	3.0
6	3.0
7	3.0
8	2.0
9	0.0
10	2.0
11	0.0
12	1.0
13	0.0
14	11.0
15	32.0
16	88.0
17	140.0
18	230.0
19	299.0
20	335.0
21	382.0
22	380.0
23	378.0
24	415.0
25	453.0
26	460.0
27	497.0
28	617.0
29	725.0
30	777.0
31	973.0
32	1303.0
33	1662.0
34	2905.0
35	3485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04255961364322	19.29972834289164	11.93480229399336	25.72290974947178
2	16.257168729248413	21.79897373981286	37.247207968608514	24.696649562330215
3	19.260958821398383	24.713198889023065	28.93370365897838	27.09213863060017
4	12.041062801932368	16.304347826086957	36.231884057971016	35.42270531400966
5	14.174417200144946	37.142166928372994	33.1682570358739	15.515158835608164
6	31.77287828450619	36.75022651766838	17.33615221987315	14.14074297795228
7	27.830815709969787	31.335347432024168	22.36858006042296	18.46525679758308
8	27.19966159052453	32.6686004350979	20.419386028523085	19.712351945854483
9	26.654578422484132	14.40314294348746	19.401631912964643	39.540646721063766
10	15.527349652462979	27.126019945602902	31.56240556059232	25.784224841341796
11	36.02127788188358	22.317596566523605	22.553345826029137	19.107779725563685
12	23.313588007736943	25.0	29.491054158607348	22.195357833655706
13	28.833947893368794	20.33488484555401	25.89614942876141	24.935017832315783
14	23.361943907156675	20.357833655705996	26.124274661508707	30.15594777562863
15	24.788442940038685	27.568907156673113	24.093326885880078	23.549323017408124
16	24.815665417623595	27.354043273298682	23.98767073612958	23.842620572948146
17	22.45995769114536	26.491387126019944	26.763372620126923	24.285282562707767
18	24.028289911140664	25.436740615366016	27.921175119385843	22.613794354107476
19	23.88781431334623	25.88249516441006	27.39966150870406	22.83002901353965
20	24.581444545179814	24.218797219703838	27.881535207011183	23.318223028105166
21	25.241779497098648	24.52248549323017	26.1968085106383	24.038926499032883
22	24.241141613254324	25.34768412141734	26.9137743378885	23.497399927439837
23	23.588441542739695	24.53149558699069	27.566195139644538	24.313867730625073
24	23.2043530834341	26.118500604594924	26.862152357920195	23.814993954050788
25	23.215041412248354	24.829212260443747	27.465086754126112	24.49065957318179
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	12.5
3	22.0
4	22.0
5	22.0
6	42.0
7	62.0
8	62.0
9	62.0
10	73.0
11	84.0
12	84.0
13	84.0
14	80.0
15	76.0
16	76.0
17	76.0
18	140.0
19	204.0
20	204.0
21	204.0
22	313.5
23	423.0
24	423.0
25	423.0
26	590.0
27	757.0
28	757.0
29	757.0
30	847.5
31	938.0
32	938.0
33	938.0
34	1077.0
35	1216.0
36	1216.0
37	1216.0
38	1276.0
39	1336.0
40	1336.0
41	1336.0
42	1510.5
43	1685.0
44	1685.0
45	1685.0
46	1919.0
47	2153.0
48	2153.0
49	2153.0
50	2091.5
51	2030.0
52	2030.0
53	2030.0
54	1827.0
55	1624.0
56	1624.0
57	1624.0
58	1454.0
59	1284.0
60	1284.0
61	1284.0
62	1136.5
63	989.0
64	989.0
65	989.0
66	848.0
67	707.0
68	707.0
69	707.0
70	545.5
71	384.0
72	384.0
73	384.0
74	331.5
75	279.0
76	279.0
77	279.0
78	220.0
79	161.0
80	161.0
81	161.0
82	122.0
83	83.0
84	83.0
85	83.0
86	59.5
87	36.0
88	36.0
89	36.0
90	24.5
91	13.0
92	13.0
93	13.0
94	8.0
95	3.0
96	3.0
97	3.0
98	8.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.018110473890733475
4	0.030184123151222455
5	0.04225777241171144
6	0.06036824630244491
7	0.09055236945366738
8	0.10262601871415634
9	0.12073649260488982
10	0.12073649260488982
11	0.1328101418653788
12	0.1267733172351343
13	0.1328101418653788
14	0.1267733172351343
15	0.1267733172351343
16	0.11469966797464533
17	0.12073649260488982
18	0.1328101418653788
19	0.1267733172351343
20	0.12073649260488982
21	0.1267733172351343
22	0.16299426501660128
23	0.1388469664956233
24	0.1509206157561123
25	0.1448837911258678
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	16565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.82915786296408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.37181851715233	75.58708119529128
2	3.9911471781630397	6.53184424992454
3	1.3574326816672815	3.3323271958949596
4	0.7082257469568425	2.318140658013885
5	0.33198081888601993	1.3582855418050106
6	0.33198081888601993	1.6299426501660126
7	0.20656584286241236	1.1832176275279205
8	0.16230173367760972	1.0624811349230303
9	0.16230173367760972	1.1952912767884092
>10	0.3614902250092217	4.750980984002415
>50	0.014754703061600885	1.0504074856625416
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	97	0.5855719891337157	No Hit
TATCAACGCAGAGTACTTTTTTTTT	77	0.46483549652882583	No Hit
GGTATCAACGCAGAGTACTTTTTTT	49	0.2958044068819801	No Hit
GTACATGGAAGCAGTGGTATCAACG	45	0.27165710836100215	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	39	0.23543616057953515	No Hit
TCCATGTACTCTGCGTTGATACCAC	33	0.1992152127980682	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	32	0.19317838816782373	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	32	0.19317838816782373	No Hit
GGTTAATTCCGATAACGAACGAGAC	26	0.15695744038635678	No Hit
GAGTACATGGAAGCAGTGGTATCAA	19	0.11469966797464533	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	19	0.11469966797464533	No Hit
GCTTTGAACACTCTAATTTTTTCAA	19	0.11469966797464533	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	18	0.10866284334440084	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	18	0.10866284334440084	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	18	0.10866284334440084	No Hit
GAATAACGCCGCCGCATCGCCAGTC	17	0.10262601871415634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE