##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16565
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.180863265922124	32.0	32.0	32.0	21.0	32.0
2	30.20911560519167	32.0	32.0	32.0	21.0	32.0
3	30.87582251735587	32.0	32.0	32.0	32.0	32.0
4	31.174162390582552	32.0	32.0	32.0	32.0	32.0
5	31.012918804708722	32.0	32.0	32.0	32.0	32.0
6	34.52997283428916	36.0	36.0	36.0	32.0	36.0
7	34.55194687594325	36.0	36.0	36.0	32.0	36.0
8	34.383398732266826	36.0	36.0	36.0	32.0	36.0
9	34.694959251433744	36.0	36.0	36.0	32.0	36.0
10	34.49954723815273	36.0	36.0	36.0	32.0	36.0
11	34.7406580138847	36.0	36.0	36.0	32.0	36.0
12	34.61086628433444	36.0	36.0	36.0	32.0	36.0
13	34.697675822517354	36.0	36.0	36.0	32.0	36.0
14	34.558889224268036	36.0	36.0	36.0	32.0	36.0
15	34.51101720495019	36.0	36.0	36.0	32.0	36.0
16	34.63199517054029	36.0	36.0	36.0	32.0	36.0
17	34.521400543314215	36.0	36.0	36.0	32.0	36.0
18	34.5951101720495	36.0	36.0	36.0	32.0	36.0
19	34.545789314820404	36.0	36.0	36.0	32.0	36.0
20	34.6245698762451	36.0	36.0	36.0	32.0	36.0
21	34.500573498339875	36.0	36.0	36.0	32.0	36.0
22	34.481074554784186	36.0	36.0	36.0	32.0	36.0
23	34.503833383640206	36.0	36.0	36.0	32.0	36.0
24	34.46755206761244	36.0	36.0	36.0	32.0	36.0
25	34.41340175067914	36.0	36.0	36.0	32.0	36.0
26	34.353214609115604	36.0	36.0	36.0	32.0	36.0
27	34.38394204648355	36.0	36.0	36.0	32.0	36.0
28	34.26694838514941	36.0	36.0	36.0	32.0	36.0
29	34.25801388469665	36.0	36.0	36.0	32.0	36.0
30	34.215454271053424	36.0	36.0	36.0	32.0	36.0
31	34.252641110775734	36.0	36.0	36.0	32.0	36.0
32	34.119166918201024	36.0	36.0	36.0	32.0	36.0
33	34.1580440688198	36.0	36.0	36.0	32.0	36.0
34	34.00549351041352	36.0	36.0	36.0	32.0	36.0
35	33.98424388771506	36.0	36.0	36.0	32.0	36.0
36	33.91964986417145	36.0	36.0	36.0	32.0	36.0
37	33.96070027165711	36.0	36.0	36.0	32.0	36.0
38	33.8543917899185	36.0	36.0	36.0	32.0	36.0
39	33.942650166012676	36.0	36.0	36.0	32.0	36.0
40	33.82390582553577	36.0	36.0	36.0	32.0	36.0
41	33.83797162692424	36.0	36.0	36.0	32.0	36.0
42	33.76220947781467	36.0	36.0	36.0	32.0	36.0
43	33.77597343797163	36.0	36.0	36.0	32.0	36.0
44	33.738364020525204	36.0	36.0	36.0	32.0	36.0
45	33.61696347721099	36.0	36.0	36.0	27.0	36.0
46	33.63760941744642	36.0	36.0	36.0	27.0	36.0
47	33.60018110473891	36.0	36.0	36.0	21.0	36.0
48	33.587322668276485	36.0	36.0	36.0	21.0	36.0
49	33.51916691820102	36.0	36.0	36.0	21.0	36.0
50	32.82915786296408	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	1.0
23	8.0
24	30.0
25	71.0
26	113.0
27	210.0
28	297.0
29	420.0
30	641.0
31	908.0
32	1301.0
33	2077.0
34	3811.0
35	6675.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82436756626215	19.724687556602063	12.063032059409528	25.387912817726253
2	17.59840618208162	21.655397247041776	35.93334943250422	24.812847138372373
3	19.939631753697558	24.67853908843948	28.53607002716571	26.845759130697257
4	13.872623000301843	17.32568668880169	35.725928161786904	33.075762149109565
5	16.233021430727437	36.01569574403863	32.24871717476607	15.502565650467853
6	32.9308783579837	36.565046785390884	17.023845457289465	13.480229399335949
7	28.837911258677934	31.614850588590404	21.014186537881073	18.53305161485059
8	27.968608511922728	33.0395412013281	19.69212194385753	19.29972834289164
9	26.97856927256263	15.116208874132205	18.436462420766677	39.468759432538484
10	15.460307878056142	26.845759130697257	31.596740114699667	26.097192876546938
11	36.48656806519771	21.74464231814066	22.20344099003924	19.5653486266224
12	23.742831270751587	24.73287051011168	28.795653486266225	22.72864473287051
13	29.067310594627227	20.790824026562028	24.847570178086325	25.29429520072442
14	23.422879565348627	20.543314216722003	24.883791125867795	31.15001509206158
15	24.835496528825836	27.159674011469964	22.710534258979777	25.29429520072442
16	24.93208572290975	27.26230003018412	23.483247811651072	24.322366435255056
17	22.56565046785391	27.141563537579234	25.783277995774224	24.509507998792635
18	24.147298520977966	25.56595230908542	26.845759130697257	23.44099003923936
19	24.310292785994566	27.370962873528526	25.016601267733172	23.302143072743736
20	24.1654089948687	26.163597947479627	25.650467853908843	24.02052520374283
21	25.680651977060066	25.910051313009358	24.147298520977966	24.26199818895261
22	24.3827346815575	25.71083610021129	25.19770600664051	24.7087232115907
23	23.773015393902806	25.777241171143977	25.33655297313613	25.113190461817087
24	23.76094174464232	27.002716571083614	24.889827950498038	24.346513733776035
25	24.213703591910654	25.8617567159674	25.016601267733172	24.90793842438877
26	23.851494114095985	26.74916993661334	25.19770600664051	24.201629942650165
27	24.90793842438877	26.537881074554786	24.853607002716572	23.70057349833987
28	23.296106248113492	26.65258074252943	26.00664050709327	24.044672502263808
29	23.646242076667672	26.9061273769997	25.24600060368246	24.201629942650165
30	23.773015393902806	26.634470268638694	26.187745246000603	23.404769091457894
31	23.658315725928162	26.362813160277693	25.439178991850287	24.539692121943858
32	23.942046483549653	27.226079082402656	24.35858738303652	24.47328705101117
33	23.471174162390582	26.489586477512827	25.692725626320556	24.346513733776035
34	24.19559311801992	26.067008753395715	26.447328705101114	23.29006942348325
35	25.48747358889224	26.073045578025962	25.276184726833684	23.16329610624811
36	23.579837005734984	26.56806519770601	24.847570178086325	25.004527618472682
37	24.684575913069725	26.067008753395715	24.99849079384244	24.24992453969212
38	24.068819800784787	25.590099607606398	25.49954723815273	24.841533353456082
39	24.123151222456986	26.447328705101114	24.726833685481438	24.70268638696046
40	24.69061273769997	26.139450648958647	25.650467853908843	23.519468759432538
41	23.954120132810143	25.680651977060066	26.71898581346212	23.646242076667672
42	24.400845155448234	26.549954723815272	25.505584062782976	23.543616057953518
43	23.380621792936914	24.99849079384244	26.471476003622097	25.14941140959855
44	22.93993359492907	26.04286145487474	26.06097192876547	24.956233021430727
45	23.61605795351645	26.441291880470875	25.964382734681557	23.978267431331123
46	23.434953214609116	26.139450648958647	26.429218231210385	23.996377905221852
47	23.205553878659824	26.217929369151825	26.41110775731965	24.1654089948687
48	23.664352550558405	27.389073347419256	25.306368849984906	23.640205252037426
49	22.47509809840024	26.869906429218233	26.537881074554786	24.117114397826743
50	22.964080893450046	26.809538182915787	25.29429520072442	24.93208572290975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	3.5
2	2.0
3	7.5
4	13.0
5	14.5
6	16.0
7	18.0
8	20.0
9	19.5
10	19.0
11	27.0
12	35.0
13	36.5
14	38.0
15	42.5
16	47.0
17	63.5
18	80.0
19	86.5
20	93.0
21	105.0
22	117.0
23	146.0
24	175.0
25	189.5
26	204.0
27	249.0
28	294.0
29	312.0
30	330.0
31	363.5
32	397.0
33	421.5
34	446.0
35	508.0
36	570.0
37	642.5
38	715.0
39	736.5
40	758.0
41	834.0
42	910.0
43	919.5
44	929.0
45	954.0
46	979.0
47	1054.0
48	1129.0
49	1141.5
50	1154.0
51	1082.0
52	1010.0
53	948.0
54	886.0
55	910.5
56	935.0
57	908.0
58	881.0
59	817.5
60	754.0
61	667.0
62	580.0
63	524.5
64	469.0
65	419.5
66	370.0
67	339.0
68	308.0
69	291.5
70	275.0
71	227.0
72	179.0
73	156.0
74	133.0
75	109.5
76	86.0
77	77.0
78	68.0
79	56.0
80	44.0
81	34.5
82	25.0
83	26.0
84	27.0
85	19.5
86	12.0
87	10.5
88	9.0
89	5.0
90	1.0
91	3.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	14.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012073649260488983
2	0.006036824630244492
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	16565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.22607908240265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.77119523842481	81.79293691518261
2	3.5158142432002215	6.133413824328404
3	1.0727385978268393	2.8071234530636886
4	0.5813551110803515	2.0283730757621488
5	0.3460447089763998	1.5092061575611229
6	0.15918056612914389	0.8330817989737399
7	0.1314969894110319	0.8028976758225174
8	0.12457609523150391	0.8693027467552067
9	0.07612983597480794	0.5976456383942046
>10	0.22146861374489585	2.626018714156354
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	29	0.17506791427709026	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	21	0.1267733172351343	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	19	0.11469966797464533	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	17	0.10262601871415634	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17	0.10262601871415634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.006036824630244492	0.0
23	0.0	0.0	0.0	0.006036824630244492	0.0
24	0.0	0.0	0.0	0.006036824630244492	0.0
25	0.0	0.0	0.0	0.006036824630244492	0.0
26	0.0	0.0	0.0	0.006036824630244492	0.0
27	0.0	0.0	0.0	0.006036824630244492	0.0
28	0.0	0.0	0.0	0.018110473890733475	0.0
29	0.0	0.0	0.0	0.04225777241171144	0.0
30	0.0	0.0	0.0	0.07847872019317839	0.0
31	0.0	0.0	0.0	0.1509206157561123	0.0
32	0.0	0.0	0.0	0.24147298520977967	0.0
33	0.0	0.0	0.0	0.3742831270751585	0.0
34	0.0	0.0	0.0	0.5010564443102928	0.0
35	0.0	0.0	0.0	0.6278297615454271	0.0
36	0.0	0.0	0.0	0.8210081497132509	0.0
37	0.0	0.0	0.0	1.068517959553275	0.0
38	0.0	0.0	0.0	1.4910956836703895	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE