FastQCFastQC Report
Thu 2 Feb 2017
SRR4062586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863581
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36810.42624837739598254No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25620.2966716497931289No Hit
TATCAACGCAGAGTACTTTTTTTTT24310.28150225630253556No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18730.21688758784642087No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15050.1742743297965101No Hit
GTACATGGAAGCAGTGGTATCAACG14810.17149520427151593No Hit
GGTATCAACGCAGAGTACTTTTTTT13100.1516939349059324No Hit
TCCATGTACTCTGCGTTGATACCAC11740.13594555693096536No Hit
GCGCAAGACGGACCAGAGCGAAAGC9940.115102115493509No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9590.11104922410289249No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9210.1066489420216517No Hit
GAACTACGACGGTATCTGATCGTCT8800.10190126924978665No Hit
GTACATGGGGTGGTATCAACGCAAA8770.10155387855916237No Hit
GATTAAGAGGGACGGCCGGGGGCAT8640.10004851889979052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGTA350.00217091616.2837284
ACCGAGT400.00527679614.2499138
GTTATCA400.005305278614.2383561
CGCATCG1350.014.07562113
CGAACGA551.9538497E-413.822103516
GAACGAG551.9538497E-413.822103517
ACGAACG551.9557732E-413.82050115
TTAATCC655.453243E-513.1522414
AGGCCCG1900.013.00142910
CGCCAGT1403.6379788E-1212.89576918
ACTCTAA1501.8189894E-1212.66805810
CTTATTC604.098967E-412.6629183
GATATAC751.4892432E-512.6563171
ATCGCCA1551.8189894E-1212.26154416
CGCGCTA701.08693086E-412.21775216
GCGCGCT701.08810855E-412.21633615
ACGCGCG701.08869775E-412.21562813
CCGATAA701.0898772E-412.2142119
GGTTCCG701.0898772E-412.2142118
GTCGGCG1251.4042598E-912.16133712
Produced by FastQC (version 0.11.5)