FastQCFastQC Report
Thu 2 Feb 2017
SRR4062586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863581
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.12332369517161679No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9950.11521791239038376No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8990.10410141029040702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA250.00235251335.19972229
TTAGTAC353.217257E-431.4283223
TCGTCTA602.872012E-421.99982627
GATATAC856.0982784E-620.7105161
TAGTACA654.930858E-420.3075314
TACGACT550.00448279719.9998444
CGCAATA3200.019.93734436
GCATATA1001.1202683E-619.8044321
TAGGACG1252.285924E-819.3598484
TAGCGGC3300.019.33318130
CTAGCGG3300.019.33318129
CGGTCCA3450.019.13028310
CAATACG3250.018.95369538
AATACGA3250.018.95369539
CCCTATA708.108552E-418.8613621
TGCGCCC708.1211905E-418.85699334
TGCACCG951.5963571E-518.526175
TCTAGCG3450.018.49260728
GCAATAC3450.018.49260737
CGTCTTA1456.2991603E-918.20675315
Produced by FastQC (version 0.11.5)