##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863581
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18228052724643	32.0	32.0	32.0	32.0	32.0
2	31.421089625640214	32.0	32.0	32.0	32.0	32.0
3	31.502255144566636	32.0	32.0	32.0	32.0	32.0
4	31.604397271361922	32.0	32.0	32.0	32.0	32.0
5	31.55116312193066	32.0	32.0	32.0	32.0	32.0
6	35.1393789349233	36.0	36.0	36.0	36.0	36.0
7	35.145194255084355	36.0	36.0	36.0	36.0	36.0
8	35.097388664178574	36.0	36.0	36.0	36.0	36.0
9	35.19692536079418	36.0	36.0	36.0	36.0	36.0
10	35.09268383625856	36.0	36.0	36.0	36.0	36.0
11	35.211705676711276	36.0	36.0	36.0	36.0	36.0
12	35.12188086583656	36.0	36.0	36.0	36.0	36.0
13	35.14831729739306	36.0	36.0	36.0	36.0	36.0
14	35.11501758375879	36.0	36.0	36.0	36.0	36.0
15	35.08256202950273	36.0	36.0	36.0	36.0	36.0
16	35.09368895332343	36.0	36.0	36.0	36.0	36.0
17	35.071964297500756	36.0	36.0	36.0	36.0	36.0
18	35.08248676151977	36.0	36.0	36.0	36.0	36.0
19	35.057817390609564	36.0	36.0	36.0	36.0	36.0
20	35.032713781336085	36.0	36.0	36.0	36.0	36.0
21	35.029433255247625	36.0	36.0	36.0	36.0	36.0
22	35.01968431449974	36.0	36.0	36.0	36.0	36.0
23	34.98621090552015	36.0	36.0	36.0	36.0	36.0
24	34.95596707199441	36.0	36.0	36.0	36.0	36.0
25	34.93898661503669	36.0	36.0	36.0	32.0	36.0
26	34.902493222986614	36.0	36.0	36.0	32.0	36.0
27	34.88441732738446	36.0	36.0	36.0	32.0	36.0
28	34.86285594518638	36.0	36.0	36.0	32.0	36.0
29	34.839301698393086	36.0	36.0	36.0	32.0	36.0
30	34.82171909757162	36.0	36.0	36.0	32.0	36.0
31	34.801469694215136	36.0	36.0	36.0	32.0	36.0
32	34.772567946724166	36.0	36.0	36.0	32.0	36.0
33	34.723195623803676	36.0	36.0	36.0	32.0	36.0
34	34.69765777616691	36.0	36.0	36.0	32.0	36.0
35	34.6608135195193	36.0	36.0	36.0	32.0	36.0
36	34.63614183267117	36.0	36.0	36.0	32.0	36.0
37	34.61104169730459	36.0	36.0	36.0	32.0	36.0
38	34.574009849684046	36.0	36.0	36.0	32.0	36.0
39	34.55474124604409	36.0	36.0	36.0	32.0	36.0
40	34.53618247738197	36.0	36.0	36.0	32.0	36.0
41	34.4841306142678	36.0	36.0	36.0	32.0	36.0
42	34.43724329275424	36.0	36.0	36.0	32.0	36.0
43	34.42582456075342	36.0	36.0	36.0	32.0	36.0
44	34.38695617434844	36.0	36.0	36.0	32.0	36.0
45	34.31701137472918	36.0	36.0	36.0	32.0	36.0
46	34.29626867659201	36.0	36.0	36.0	32.0	36.0
47	34.24264544958724	36.0	36.0	36.0	32.0	36.0
48	34.1897401633431	36.0	36.0	36.0	32.0	36.0
49	34.17188659778295	36.0	36.0	36.0	32.0	36.0
50	33.661615992014646	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	9.0
21	53.0
22	78.0
23	283.0
24	704.0
25	1797.0
26	3558.0
27	6289.0
28	10021.0
29	15152.0
30	21170.0
31	29391.0
32	42140.0
33	65761.0
34	139525.0
35	527645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.497976153939696	18.074248223705926	11.504479098505422	25.923296523848954
2	16.527472832884023	20.631953901596212	36.03059021891183	26.809983046607933
3	18.853508790213695	24.439596375064557	28.405209049832443	28.301685784889308
4	12.617345680370457	15.702059216217123	35.19970911819505	36.48088598521737
5	14.489434112144664	36.528015322245395	32.91318359250609	16.069366973103854
6	34.37033914791518	34.73232234337221	16.663501533159792	14.23383697555282
7	29.88903183372492	30.749055386813744	20.79341717800646	18.568495601454874
8	28.704545375593028	31.729044525064815	19.761782623749248	19.80462747559291
9	27.63006596949215	13.961979246880144	19.003660339910212	39.4042944437175
10	16.458328749706165	26.439442275825893	31.05140108455374	26.050827889914206
11	37.14660234535035	21.43608995566137	21.9222053287416	19.49510237024668
12	24.851287835188593	24.097565833430796	28.262896010912698	22.788250320467913
13	29.57093775800996	19.43836189077805	25.528236494318424	25.462463856893564
14	23.864350883125034	19.679914217658794	25.339024364825068	31.1167105343911
15	25.34678275691568	26.62089601322864	23.094764706495397	24.93755652336029
16	25.614389385593245	25.71802760829615	24.042099119827785	24.625483886282815
17	23.754575424887765	25.707258496886798	25.607094181090133	24.9310718971353
18	24.857193476929204	24.073943266468344	27.086167944871413	23.982695311731035
19	25.269893617390842	25.014214069091377	26.210280216910746	23.505612096607035
20	25.45018938582484	23.825906313362616	26.105715619032843	24.618188681779706
21	26.710870202100324	23.912985579812432	25.154791501897332	24.221352716189912
22	25.637780358761947	24.139831700790083	25.579302925840192	24.64308501460778
23	24.15117979668381	23.922596722253036	26.36429008975417	25.561933391308976
24	24.487917172795605	24.951683744779007	25.998487692526815	24.561911389898576
25	24.75899770837941	24.098029021018295	26.154234518823365	24.98873875177893
26	24.212783745821177	25.021161882903865	26.607231979397415	24.158822391877543
27	24.96604255999148	24.701215056838908	25.800474998870982	24.53226738429864
28	24.18267655263374	24.38752126320519	26.63073874946299	24.799063434698077
29	24.06965878128398	24.87525779284167	26.58882027279433	24.46626315308002
30	24.132883886977595	24.887648060807265	26.689910963765996	24.289557088449143
31	24.400374718758286	24.75320786353567	25.759714491171064	25.086702926534976
32	24.196803774052462	24.703878385467025	25.850499258320873	25.248818582159636
33	24.00052803384975	24.282493477739784	26.30430729717305	25.41267119123742
34	24.602556100701616	24.426776411245733	26.63097034325674	24.339697144795913
35	25.256576974250244	24.504707722842443	26.22857612661696	24.010139176290355
36	24.039898978787168	24.978432827957075	26.070050174795416	24.91161801846034
37	25.13163212252238	24.80960095231368	25.749292770452335	24.3094741547116
38	24.0489311367434	24.630347355951553	26.393702501560362	24.927019005744683
39	24.43117669332697	24.539794182595497	25.83382450517091	25.195204618906626
40	24.68720363231706	24.674118582970213	26.430757508560287	24.20792027615244
41	23.60728177206307	24.918565832272826	26.819024503781346	24.655127891882753
42	24.936282757494666	25.002634379403897	26.274663291573113	23.786419571528324
43	24.01708699010284	24.197035367846212	26.29863324922619	25.48724439282476
44	23.41146921944786	24.7874837450106	26.473139172816445	25.327907862725098
45	23.6181666803693	24.892164139785383	26.50973099222887	24.979938187616447
46	23.374699940596056	24.68830298096642	26.591313591460654	25.34568348697687
47	23.621756384172418	24.559016467476706	26.876922952218727	24.942304196132152
48	24.181981771252495	25.700194886177442	25.599798976587024	24.51802436598304
49	23.048330150848617	25.48342309522789	26.162687692295222	25.30555906162827
50	22.98139954445501	25.942673588233184	25.875048200458323	25.200878666853487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	105.5
2	93.0
3	285.0
4	477.0
5	516.5
6	556.0
7	451.5
8	347.0
9	480.0
10	613.0
11	936.5
12	1260.0
13	2040.5
14	2821.0
15	3678.5
16	4536.0
17	4858.0
18	5180.0
19	5336.0
20	5492.0
21	5979.0
22	6466.0
23	6419.5
24	6373.0
25	6679.0
26	6985.0
27	8021.5
28	9058.0
29	10264.5
30	11471.0
31	12519.0
32	13567.0
33	15572.0
34	17577.0
35	19859.5
36	22142.0
37	26311.0
38	30480.0
39	33052.5
40	35625.0
41	39057.0
42	42489.0
43	43997.0
44	45505.0
45	50094.0
46	54683.0
47	58599.0
48	62515.0
49	63670.0
50	64825.0
51	61715.0
52	58605.0
53	57892.5
54	57180.0
55	58325.5
56	59471.0
57	58202.0
58	56933.0
59	52342.0
60	47751.0
61	42262.5
62	36774.0
63	32273.5
64	27773.0
65	23955.5
66	20138.0
67	17611.0
68	15084.0
69	13427.0
70	11770.0
71	9288.5
72	6807.0
73	6149.5
74	5492.0
75	4062.0
76	2632.0
77	2339.0
78	2046.0
79	1836.5
80	1627.0
81	1263.0
82	899.0
83	771.5
84	644.0
85	538.0
86	432.0
87	306.0
88	180.0
89	116.5
90	53.0
91	36.0
92	19.0
93	14.0
94	9.0
95	5.0
96	1.0
97	2.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014590409006219452
2	0.00428448518436603
3	8.105782781233028E-4
4	0.0
5	0.0
6	5.789844843737878E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.3159379374951508E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	863581.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.22923581764547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.47191149943978	49.67220206031745
2	11.224507231043376	13.520869859107565
3	2.8669907681766476	5.180299891905714
4	1.134642435084268	2.7335458716559144
5	0.5770072657270648	1.7376353337985107
6	0.33817790194629826	1.222091796278411
7	0.23212705759213065	0.9786584703960824
8	0.16421082511667506	0.7912234007809889
9	0.12540000124451117	0.6797471621839828
>10	0.6925619914847749	8.148504251625166
>50	0.0827528202658558	3.5682902586090948
>100	0.08468333807701948	9.678906516989422
>500	0.004833523847706682	1.9640082416511835
>1k	1.9334095390826728E-4	0.12401688470049271
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1065	0.12332369517161679	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	995	0.11521791239038376	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	899	0.10410141029040702	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1579689687475755E-4	0.0
5	0.0	0.0	0.0	1.1579689687475755E-4	1.1579689687475755E-4
6	0.0	0.0	0.0	1.1579689687475755E-4	1.1579689687475755E-4
7	0.0	0.0	0.0	2.315937937495151E-4	1.1579689687475755E-4
8	0.0	0.0	0.0	2.315937937495151E-4	1.1579689687475755E-4
9	0.0	0.0	0.0	3.4739069062427266E-4	1.1579689687475755E-4
10	0.0	0.0	0.0	3.4739069062427266E-4	1.1579689687475755E-4
11	0.0	0.0	0.0	3.4739069062427266E-4	1.1579689687475755E-4
12	0.0	0.0	0.0	3.4739069062427266E-4	1.1579689687475755E-4
13	0.0	0.0	0.0	4.631875874990302E-4	1.1579689687475755E-4
14	0.0	0.0	0.0	4.631875874990302E-4	1.1579689687475755E-4
15	0.0	1.1579689687475755E-4	0.0	5.789844843737878E-4	4.631875874990302E-4
16	0.0	1.1579689687475755E-4	0.0	6.947813812485453E-4	4.631875874990302E-4
17	0.0	1.1579689687475755E-4	0.0	8.105782781233029E-4	4.631875874990302E-4
18	0.0	1.1579689687475755E-4	0.0	8.105782781233029E-4	4.631875874990302E-4
19	0.0	1.1579689687475755E-4	0.0	9.263751749980604E-4	4.631875874990302E-4
20	0.0	1.1579689687475755E-4	0.0	0.0013895627624970906	4.631875874990302E-4
21	0.0	1.1579689687475755E-4	0.0	0.0017369534531213632	4.631875874990302E-4
22	0.0	1.1579689687475755E-4	0.0	0.002547531731244666	4.631875874990302E-4
23	0.0	1.1579689687475755E-4	0.0	0.0048634696687398175	4.631875874990302E-4
24	0.0	1.1579689687475755E-4	0.0	0.00798998588435827	4.631875874990302E-4
25	0.0	1.1579689687475755E-4	0.0	0.010190126924978664	4.631875874990302E-4
26	0.0	1.1579689687475755E-4	0.0	0.014474612109344694	4.631875874990302E-4
27	0.0	1.1579689687475755E-4	0.0	0.02223300419995345	4.631875874990302E-4
28	0.0	1.1579689687475755E-4	0.0	0.040413117009290386	4.631875874990302E-4
29	0.0	1.1579689687475755E-4	0.0	0.06843596605298172	4.631875874990302E-4
30	0.0	1.1579689687475755E-4	0.0	0.12934513380910417	4.631875874990302E-4
31	0.0	1.1579689687475755E-4	0.0	0.24722637482760737	4.631875874990302E-4
32	0.0	1.1579689687475755E-4	0.0	0.39162510523043004	5.789844843737878E-4
33	0.0	1.1579689687475755E-4	0.0	0.5344026790770061	5.789844843737878E-4
34	0.0	4.631875874990302E-4	0.0	0.6928128340016744	6.947813812485453E-4
35	0.0	4.631875874990302E-4	0.0	0.8753087434762923	6.947813812485453E-4
36	0.0	4.631875874990302E-4	0.0	1.1263564159007666	6.947813812485453E-4
37	0.0	8.105782781233029E-4	0.0	1.4979486579718637	6.947813812485453E-4
38	0.0	8.105782781233029E-4	0.0	1.9543042285552832	6.947813812485453E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	25	0.002352513	35.199722	29
TTAGTAC	35	3.217257E-4	31.428322	3
TCGTCTA	60	2.872012E-4	21.999826	27
GATATAC	85	6.0982784E-6	20.710516	1
TAGTACA	65	4.930858E-4	20.307531	4
TACGACT	55	0.004482797	19.999844	4
CGCAATA	320	0.0	19.937344	36
GCATATA	100	1.1202683E-6	19.804432	1
TAGGACG	125	2.285924E-8	19.359848	4
TAGCGGC	330	0.0	19.333181	30
CTAGCGG	330	0.0	19.333181	29
CGGTCCA	345	0.0	19.130283	10
CAATACG	325	0.0	18.953695	38
AATACGA	325	0.0	18.953695	39
CCCTATA	70	8.108552E-4	18.861362	1
TGCGCCC	70	8.1211905E-4	18.856993	34
TGCACCG	95	1.5963571E-5	18.52617	5
TCTAGCG	345	0.0	18.492607	28
GCAATAC	345	0.0	18.492607	37
CGTCTTA	145	6.2991603E-9	18.206753	15
>>END_MODULE