FastQCFastQC Report
Thu 2 Feb 2017
SRR4062585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754261
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG72670.414248506921148No Hit
TCCATGTACTCTGCGTTGATACCAC61200.3488648496432401No Hit
GTATCAACGCAGAGTACTTTTTTTT37340.21285316153069586No Hit
GAGTACATGGAAGCAGTGGTATCAA33070.18851242774022792No Hit
CATGTACTCTGCGTTGATACCACTG28720.16371566146656627No Hit
TATCAACGCAGAGTACTTTTTTTTT25560.14570237838041203No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22130.12614998566347882No Hit
GCGCAAGACGGACCAGAGCGAAAGC21940.12506690851589358No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21790.12421184760990525No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20600.11742836442239782No Hit
GAATAGGACCGCGGTTCTATTTTGT19690.1122409949260686No Hit
GAATAACGCCGCCGCATCGCCAGTC19440.11081589341608802No Hit
GCTTCCATGTACTCTGCGTTGATAC19000.10830771475852224No Hit
GGGTAGGCACACGCTGAGCCAGTCA18050.10289232902059614No Hit
GAACTACGACGGTATCTGATCGTCT17850.10175224781261169No Hit
GCGTTGATACCACTGCTTCCATGTA17830.10163823969181324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1550.015.32458315
TATAAGG902.3046596E-914.7700142
CCGTCGA400.00527588314.25145513
CGCCAGT3800.013.50253518
TCGCGTA1700.012.8542549
CGCATCG3750.012.6679613
GCGTTAT1900.012.4934321
CTATACT1155.342372E-912.38867954
GTGTTAT701.0964199E-412.2078681
ATCGCCA4000.012.11477516
GTCGCGT1750.011.9440778
CGTTATT2000.011.868762
GCATCGC4050.011.72959314
TCCAACG658.0116873E-411.69450318
GGTCGGC2200.011.65994911
CGCGTAA1800.011.61229710
AAGACGG4750.011.5991985
TCGTCGC1157.064773E-811.56639913
CTCGTAG1657.2759576E-1211.51632810
AGAATTT4800.011.48164917
Produced by FastQC (version 0.11.5)