FastQCFastQC Report
Thu 2 Feb 2017
SRR4062585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754261
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30800.1755725060296045No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27060.15425298744029536No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22180.12643500596547494No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20990.11965152277796746No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20210.11520520606682813No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19790.1128110355300608No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18030.10277832089979769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCTA407.0336735E-427.4998226
TACGTGT602.87362E-421.9998576
GGCGTTA4800.019.70820642
ATACCGA1152.0228254E-719.1303126
TAGGACG3000.019.0665444
TCGTATA708.125674E-418.8570244
TTAACGG2003.6379788E-1217.59988635
TAACGGC1954.9112714E-1116.92296636
CGTTATT5800.016.68954744
GCGTTAT5750.016.45206843
CGGCGTT5800.016.3102441
GTGCGAA953.3475558E-416.2104211
CGAGCCG6800.016.17636715
GTATTAG5450.016.1499061
GTAATAC2051.05501385E-1016.0979163
GATATAC1653.1055606E-816.003091
AGGACGT3600.015.8887865
AATAGCG3750.015.8398985
GGACCGT1401.587232E-615.7141836
CGACGGT6450.015.6898217
Produced by FastQC (version 0.11.5)