##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1754261
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29537907985186	32.0	32.0	32.0	32.0	32.0
2	31.44446122897334	32.0	32.0	32.0	32.0	32.0
3	31.51847245079267	32.0	32.0	32.0	32.0	32.0
4	31.616025779516274	32.0	32.0	32.0	32.0	32.0
5	31.562033243627944	32.0	32.0	32.0	32.0	32.0
6	35.16264170496864	36.0	36.0	36.0	36.0	36.0
7	35.1748297431226	36.0	36.0	36.0	36.0	36.0
8	35.12455672217532	36.0	36.0	36.0	36.0	36.0
9	35.23856427293316	36.0	36.0	36.0	36.0	36.0
10	35.12652222217788	36.0	36.0	36.0	36.0	36.0
11	35.24355440838051	36.0	36.0	36.0	36.0	36.0
12	35.159349150439986	36.0	36.0	36.0	36.0	36.0
13	35.201365703279045	36.0	36.0	36.0	36.0	36.0
14	35.16186188942238	36.0	36.0	36.0	36.0	36.0
15	35.127918821657666	36.0	36.0	36.0	36.0	36.0
16	35.13167253903496	36.0	36.0	36.0	36.0	36.0
17	35.10490685251511	36.0	36.0	36.0	36.0	36.0
18	35.12204683339594	36.0	36.0	36.0	36.0	36.0
19	35.098141610626925	36.0	36.0	36.0	36.0	36.0
20	35.09083996053039	36.0	36.0	36.0	36.0	36.0
21	35.07629936480375	36.0	36.0	36.0	36.0	36.0
22	35.06234762102105	36.0	36.0	36.0	36.0	36.0
23	35.01181466155834	36.0	36.0	36.0	36.0	36.0
24	34.99391481655238	36.0	36.0	36.0	36.0	36.0
25	34.95807864394181	36.0	36.0	36.0	32.0	36.0
26	34.918453411436495	36.0	36.0	36.0	32.0	36.0
27	34.89284661746456	36.0	36.0	36.0	32.0	36.0
28	34.86655577476784	36.0	36.0	36.0	32.0	36.0
29	34.843113995009865	36.0	36.0	36.0	32.0	36.0
30	34.83007773643717	36.0	36.0	36.0	32.0	36.0
31	34.819154618383465	36.0	36.0	36.0	32.0	36.0
32	34.78976731512586	36.0	36.0	36.0	32.0	36.0
33	34.75917095574718	36.0	36.0	36.0	32.0	36.0
34	34.747781544479416	36.0	36.0	36.0	32.0	36.0
35	34.70862317522877	36.0	36.0	36.0	32.0	36.0
36	34.68019411022647	36.0	36.0	36.0	32.0	36.0
37	34.6547389470552	36.0	36.0	36.0	32.0	36.0
38	34.60598565435816	36.0	36.0	36.0	32.0	36.0
39	34.57078792722405	36.0	36.0	36.0	32.0	36.0
40	34.537753504182106	36.0	36.0	36.0	32.0	36.0
41	34.520448781566714	36.0	36.0	36.0	32.0	36.0
42	34.44270436383184	36.0	36.0	36.0	32.0	36.0
43	34.46383063865639	36.0	36.0	36.0	32.0	36.0
44	34.42872240789711	36.0	36.0	36.0	32.0	36.0
45	34.34979230570593	36.0	36.0	36.0	32.0	36.0
46	34.391634996160775	36.0	36.0	36.0	32.0	36.0
47	34.331066471864794	36.0	36.0	36.0	32.0	36.0
48	34.30916095153458	36.0	36.0	36.0	32.0	36.0
49	34.31797548939411	36.0	36.0	36.0	32.0	36.0
50	33.79106985790598	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	8.0
21	40.0
22	155.0
23	463.0
24	1229.0
25	2829.0
26	6270.0
27	11839.0
28	19465.0
29	29263.0
30	40785.0
31	57083.0
32	83985.0
33	129955.0
34	288390.0
35	1082501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3446834009039	17.750883852565238	11.668564267669497	26.23586847886137
2	16.254815601923163	20.213100717480508	36.048008373076186	27.48407530752015
3	18.905151597375973	23.547244067557	28.328997202226365	29.218607132840663
4	12.516794840442602	15.351381979513857	35.30653113365814	36.8252920463854
5	14.42477169860796	36.48780682453	33.36113232930125	15.726289147560795
6	35.34045202068569	34.626018095420505	16.27225444860955	13.761275435284245
7	30.579885205223167	30.23387055859989	20.435784640939975	18.75045959523697
8	28.72839332345643	32.491003334167495	19.315540845974457	19.465062496401618
9	27.15342813868632	14.236764084705753	18.535383275350704	40.07442450125722
10	16.052058388118986	26.601799846203043	31.307371024038044	26.038770741639926
11	37.96436220151961	20.965409366109146	21.492468908560355	19.57775952381088
12	24.475719405493255	23.67646547463576	28.345953082238047	23.50186203763294
13	29.76159191819233	19.021000865891676	25.213465955179988	26.003941260736003
14	23.70183228151341	19.491113352004064	24.60204040333793	32.205013963144594
15	25.558055500293285	26.85028054548326	22.49203510766072	25.099628846562737
16	26.37640579138452	25.462915723486983	23.63451048618193	24.526167998946566
17	24.17262881635059	25.78709781497736	25.349591651413327	24.69068171725872
18	25.497346175968115	24.07207365380636	26.509510272416705	23.92106989780882
19	25.520717840731795	25.54004221720713	25.017485995527462	23.92175394653361
20	25.53365776244242	23.92768236881513	25.616712678444088	24.921947190298365
21	26.58692178643885	24.420767491268403	24.090770985617304	24.901539736675442
22	25.427402193858267	24.504449451934462	24.87098556030146	25.197162793905807
23	24.123719332528058	24.064378105652466	25.44176721707887	26.370135344740607
24	24.545207355119906	25.418680572617188	25.03886251817717	24.997249554085737
25	25.25274175279505	24.272442926109626	24.897321436205903	25.577493884889417
26	24.460955353849855	25.03464421770763	25.563128861668815	24.9412715667737
27	25.709914317196812	24.79249096913173	24.790666839198956	24.7069278744725
28	24.671528352964582	24.12468840155484	25.5863295142513	25.617453731229272
29	24.377330397244197	24.95466752096752	25.784475628198994	24.88352645358929
30	24.90991933355413	24.777555905307135	25.815941869539365	24.49658289159937
31	24.780520116447892	24.765186024200503	24.755723350174232	25.698570509177372
32	24.658930455616353	24.7580035125902	24.84772790365858	25.735338128134867
33	24.13141488068195	24.4209385034496	25.465195885902954	25.982450729965496
34	24.86488612583874	24.702139533398963	25.832131022692746	24.600843318069547
35	25.840225599269434	24.48495406327793	25.408419841745328	24.26640049570731
36	24.54497933887831	25.20577040702609	25.099058805958745	25.150191448136848
37	25.771307690246775	24.75230310655028	24.996337489119348	24.480051714083594
38	24.743524481248798	24.53152638062409	25.224695755078635	25.500253383048477
39	25.122430470722428	24.47064604411772	25.049921305894618	25.35700217926523
40	25.482582124324715	24.60739878501546	25.212952918636393	24.697066172023433
41	24.104850988535915	24.941784603317295	25.930520030941807	25.02284437720499
42	25.959820117987004	25.035955311096806	25.093700424281224	23.910524146634966
43	24.95472452502792	23.883447217945335	25.310600874100263	25.851227382926485
44	24.56390468693085	24.50804070773961	25.643732603073317	25.28432200225622
45	24.63880802229543	24.87754102724737	25.363386634029943	25.12026431642726
46	24.294916374995726	24.428818989203425	25.858139614424314	25.418125021376536
47	24.693930948701475	24.240292636044465	26.173243320121692	24.892533095132364
48	25.25137365534547	25.603658748612663	24.616348422498135	24.52861917354373
49	24.08968790846972	25.04023061562675	25.65672952884434	25.213351947059188
50	23.97935538594928	25.865279870304214	24.7826005167994	25.372764226947105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	14.5
2	19.0
3	69.5
4	120.0
5	178.5
6	237.0
7	225.0
8	213.0
9	302.5
10	392.0
11	592.0
12	792.0
13	1434.5
14	2077.0
15	2918.5
16	3760.0
17	4491.0
18	5222.0
19	5607.0
20	5992.0
21	6801.5
22	7611.0
23	8262.0
24	8913.0
25	10030.5
26	11148.0
27	13682.5
28	16217.0
29	18398.5
30	20580.0
31	22871.0
32	25162.0
33	29132.5
34	33103.0
35	38090.5
36	43078.0
37	52309.5
38	61541.0
39	66775.5
40	72010.0
41	79707.0
42	87404.0
43	91359.0
44	95314.0
45	107757.5
46	120201.0
47	130405.5
48	140610.0
49	141705.0
50	142800.0
51	134468.5
52	126137.0
53	123466.0
54	120795.0
55	122793.0
56	124791.0
57	121067.5
58	117344.0
59	108213.0
60	99082.0
61	87055.5
62	75029.0
63	64952.5
64	54876.0
65	46925.5
66	38975.0
67	34121.0
68	29267.0
69	26980.0
70	24693.0
71	18965.0
72	13237.0
73	11902.5
74	10568.0
75	7536.5
76	4505.0
77	4181.0
78	3857.0
79	3275.5
80	2694.0
81	2179.0
82	1664.0
83	1369.0
84	1074.0
85	882.0
86	690.0
87	515.5
88	341.0
89	207.0
90	73.0
91	48.0
92	23.0
93	16.0
94	9.0
95	7.5
96	6.0
97	4.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01664518563657289
2	0.0038192720467478897
3	5.130365435930002E-4
4	1.1400812079844447E-4
5	5.7004060399222236E-5
6	2.8502030199611115E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.7004060399222236E-5
47	0.0
48	0.0
49	0.0
50	1.1400812079844447E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1754261.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.05887928058789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62064631941963	41.715233820238836
2	12.906002775322165	13.695560865015022
3	3.7113745785479177	5.907641271846502
4	1.5886863723930567	3.3717567379007316
5	0.8240234237862835	2.186087968352656
6	0.47474089830843	1.5113532007742907
7	0.32705834285926716	1.2147344392035286
8	0.23937314705797613	1.0160696730210856
9	0.17276699224853329	0.8250140686846673
>10	0.95946301625309	9.161922957037783
>50	0.08005871778565089	3.0368222678185646
>100	0.0810083927456907	9.365135153839638
>500	0.011124769321550557	4.0645381728135925
>1k	0.0036722539508030967	2.9281294034530916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3080	0.1755725060296045	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2706	0.15425298744029536	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2218	0.12643500596547494	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2099	0.11965152277796746	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2021	0.11520520606682813	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1979	0.1128110355300608	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1803	0.10277832089979769	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.7004060399222236E-5
2	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
3	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
4	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
5	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
6	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
7	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
8	0.0	0.0	0.0	5.7004060399222236E-5	5.7004060399222236E-5
9	0.0	0.0	0.0	1.1400812079844447E-4	5.7004060399222236E-5
10	0.0	0.0	0.0	1.1400812079844447E-4	5.7004060399222236E-5
11	0.0	0.0	0.0	1.1400812079844447E-4	5.7004060399222236E-5
12	0.0	0.0	0.0	1.7101218119766672E-4	3.4202436239533343E-4
13	0.0	0.0	0.0	1.7101218119766672E-4	3.4202436239533343E-4
14	0.0	0.0	0.0	2.2801624159688895E-4	3.4202436239533343E-4
15	0.0	0.0	0.0	3.4202436239533343E-4	4.560324831937779E-4
16	0.0	0.0	0.0	5.130365435930002E-4	5.130365435930002E-4
17	0.0	0.0	0.0	5.130365435930002E-4	5.130365435930002E-4
18	0.0	0.0	0.0	6.270446643914446E-4	5.700406039922224E-4
19	0.0	0.0	0.0	8.550609059883336E-4	5.700406039922224E-4
20	0.0	0.0	0.0	0.0010830771475852225	6.270446643914446E-4
21	0.0	0.0	0.0	0.0013110933891821114	6.270446643914446E-4
22	0.0	0.0	0.0	0.002166154295170445	6.840487247906669E-4
23	0.0	0.0	0.0	0.0030782192615580006	6.840487247906669E-4
24	0.0	0.0	0.0	0.004731337013135445	6.840487247906669E-4
25	0.0	0.0	0.0	0.00587141822111989	6.840487247906669E-4
26	0.0	0.0	0.0	0.007752552214294224	7.410527851898891E-4
27	0.0	0.0	0.0	0.010545751173856114	7.410527851898891E-4
28	0.0	0.0	0.0	0.018298303388150337	7.410527851898891E-4
29	0.0	0.0	0.0	0.029984135769990896	7.410527851898891E-4
30	0.0	0.0	0.0	0.05261474774848213	7.410527851898891E-4
31	0.0	0.0	0.0	0.10089718690662336	7.410527851898891E-4
32	0.0	0.0	0.0	0.1630316127417756	7.410527851898891E-4
33	0.0	0.0	0.0	0.22197381119457138	7.410527851898891E-4
34	0.0	0.0	0.0	0.2939699394787891	7.980568455891113E-4
35	0.0	0.0	0.0	0.36893027890376634	7.980568455891113E-4
36	0.0	0.0	0.0	0.48333742812500535	7.980568455891113E-4
37	0.0	0.0	0.0	0.6590239422754083	7.980568455891113E-4
38	0.0	0.0	0.0	0.8738152418596776	8.550609059883336E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCTA	40	7.0336735E-4	27.49982	26
TACGTGT	60	2.87362E-4	21.999857	6
GGCGTTA	480	0.0	19.708206	42
ATACCGA	115	2.0228254E-7	19.130312	6
TAGGACG	300	0.0	19.066544	4
TCGTATA	70	8.125674E-4	18.85702	44
TTAACGG	200	3.6379788E-12	17.599886	35
TAACGGC	195	4.9112714E-11	16.922966	36
CGTTATT	580	0.0	16.689547	44
GCGTTAT	575	0.0	16.452068	43
CGGCGTT	580	0.0	16.31024	41
GTGCGAA	95	3.3475558E-4	16.21042	11
CGAGCCG	680	0.0	16.176367	15
GTATTAG	545	0.0	16.149906	1
GTAATAC	205	1.05501385E-10	16.097916	3
GATATAC	165	3.1055606E-8	16.00309	1
AGGACGT	360	0.0	15.888786	5
AATAGCG	375	0.0	15.839898	5
GGACCGT	140	1.587232E-6	15.714183	6
CGACGGT	645	0.0	15.689821	7
>>END_MODULE