FastQCFastQC Report
Thu 2 Feb 2017
SRR4062584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464285
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG131882.8404966776871965No Hit
TCCATGTACTCTGCGTTGATACCAC106902.3024650807155087No Hit
GAGTACATGGAAGCAGTGGTATCAA61681.3284943515297716No Hit
CATGTACTCTGCGTTGATACCACTG51031.099109383245205No Hit
GCTTCCATGTACTCTGCGTTGATAC35050.7549242383449821No Hit
GCGTTGATACCACTGCTTCCATGTA31750.6838472059187783No Hit
CATGGAAGCAGTGGTATCAACGCAG29270.6304317391257525No Hit
CCCATGTACTCTGCGTTGATACCAC28140.6060932401434463No Hit
GTACATGGGAAGCAGTGGTATCAAC25240.5436316055870855No Hit
GTATCAACGCAGAGTACATGGAAGC23750.5115392485219208No Hit
CAGTGGTATCAACGCAGAGTACATG20180.4346468225335731No Hit
ACGCAGAGTACATGGAAGCAGTGGT20000.43076989349214384No Hit
ACTCTGCGTTGATACCACTGCTTCC19830.4271083493974606No Hit
GTATCAACGCAGAGTACTTTTTTTT17460.37606211701864156No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14110.30390815985870745No Hit
TATCAACGCAGAGTACATGGAAGCA13720.29550814693561067No Hit
TATCAACGCAGAGTACTTTTTTTTT11190.24101575540885445No Hit
GTATCAACGCAGAGTACATGGGAAG10740.23132343280528123No Hit
GGTATCAACGCAGAGTACATGGAAG10680.23003112312480484No Hit
ATACCACTGCTTCCATGTACTCTGC10490.22593880913662945No Hit
GTGGTATCAACGCAGAGTACATGGA9610.2069849338229751No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9560.20590800908924478No Hit
AAGCAGTGGTATCAACGCAGAGTAC9530.20526185424900653No Hit
AAAAAGTACTCTGCGTTGATACCAC9530.20526185424900653No Hit
GAGTACATGGGAAGCAGTGGTATCA9360.2016003101543233No Hit
GTACATGGGGTGGTATCAACGCAAA8350.17984643053297006No Hit
GTACTCTGCGTTGATACCACTGCTT8230.1772618111720172No Hit
CCACTGCTTCCATGTACTCTGCGTT8130.17510796170455645No Hit
GCGTTGATACCACTGCTTCCCATGT7880.16972333803590467No Hit
GCTTCCCATGTACTCTGCGTTGATA7560.1628310197400304No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7380.15895409069860109No Hit
ACATGGAAGCAGTGGTATCAACGCA7370.158738705751855No Hit
GAAGCAGTGGTATCAACGCAGAGTA7290.15701562617788642No Hit
GGTATCAACGCAGAGTACTTTTTTT7130.15356946702994928No Hit
CTGCTTCCATGTACTCTGCGTTGAT6730.1449540691601064No Hit
ACGCAGAGTACATGGGAAGCAGTGG6140.13224635730208814No Hit
GTTGATACCACTGCTTCCATGTACT6090.1311694325683578No Hit
TGGAAGCAGTGGTATCAACGCAGAG6070.13073866267486564No Hit
TATCAACGCAGAGTACATGGGAAGC5780.12449249921922957No Hit
GATACCACTGCTTCCATGTACTCTG5490.11824633576359349No Hit
CCCCATGTACTCTGCGTTGATACCA5370.11566171640264061No Hit
GTACATGGGTGGTATCAACGCAAAA5360.11544633145589454No Hit
GGTATCAACGCAGAGTACATGGGAA5250.11307709704168777No Hit
GCAGAGTACATGGAAGCAGTGGTAT5010.10790785831978203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCACT250.00601813219.00420817
TGGATAG250.00602563719.0001095
TCTAGAT350.002174849416.2770292
GTCTTAG651.923454E-716.0631541
CGACGGT508.692517E-515.2000887
GGTTCTA456.743865E-414.77945613
AGACGGA456.7493843E-414.7778626
TCCTATT456.777037E-414.7698972
GCCGGTC653.355848E-614.6170448
GACGGTA602.5578589E-514.2516188
GACTTTC400.005267741614.25161714
TATACTG400.005271513514.2500825
TACGACG602.5604628E-514.2500815
CTACGAC602.563069E-514.2485454
CTATTCC551.9571827E-413.8167714
CGGTTCT500.001496716913.300076512
GGGTAGG655.4803008E-513.1425811
CGGTCCA604.0784993E-412.66810510
ACGGTAT604.0784993E-412.6681059
CGCCGGT604.0822133E-412.66673857
Produced by FastQC (version 0.11.5)